Construct: ORF ccsbBroad304_13904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015388.1_s304c1, BRDN0000462410
- Derived from:
- ccsbBroadEn_13904
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MTR (4548)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13904
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4548 | MTR | 5-methyltetrahydrofolate-ho... | NM_000254.2 | 99.6% | 38.8% | (many diffs) |
| 2 | human | 4548 | MTR | 5-methyltetrahydrofolate-ho... | XM_005273141.5 | 99.5% | 38.7% | (many diffs) |
| 3 | human | 4548 | MTR | 5-methyltetrahydrofolate-ho... | NM_001291939.1 | 95.6% | 40.4% | (many diffs) |
| 4 | human | 4548 | MTR | 5-methyltetrahydrofolate-ho... | XM_011544194.3 | 91.9% | 30.1% | (many diffs) |
| 5 | human | 4548 | MTR | 5-methyltetrahydrofolate-ho... | XM_017001329.2 | 88.1% | 31.2% | (many diffs) |
| 6 | human | 4548 | MTR | 5-methyltetrahydrofolate-ho... | XM_017001330.2 | 87.2% | 31.5% | (many diffs) |
| 7 | human | 4548 | MTR | 5-methyltetrahydrofolate-ho... | XM_006711770.3 | 75% | 14.1% | (many diffs) |
| 8 | human | 4548 | MTR | 5-methyltetrahydrofolate-ho... | NM_001291940.2 | 67.5% | 6.6% | (many diffs) |
| 9 | mouse | 238505 | Mtr | 5-methyltetrahydrofolate-ho... | NM_001081128.3 | 87.3% | 34.9% | (many diffs) |
| 10 | mouse | 238505 | Mtr | 5-methyltetrahydrofolate-ho... | XM_006516661.3 | 69.7% | 16.6% | (many diffs) |
| 11 | mouse | 238505 | Mtr | 5-methyltetrahydrofolate-ho... | XM_006516662.3 | 69.3% | 44% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1554
- ORF length:
- 1485
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcacccgcg ctccaagacc tgtcgcaacc cgaaggtctg aagaaaaccc 121 tgcgggatga gatcaatgcc attctgcaga agaggattat ggtgctggat ggagggatgg 181 ggaccatgat ccagcgggag aagctaaacg aagaacactt ccgaggtcag gaatttaaag 241 atcatgccag gccgctgaaa ggcaacaatg acattttaag tataactcag cctgatgtca 301 tttaccaaat ccataaggaa tacttgctgg ctggggcaga tatcattgaa acaaatactt 361 ttagcagcac tagtattgcc caagctgact atggccttga acacttggcc taccggatga 421 acatgtgctc tgcaggagtg gccagaaaag ctgccgagga ggtaactctc cagacaggaa 481 ttaagaggtt tgtggcaggg gctctgggtc cgactaataa gacactctct gtgtccccat 541 ctgtggaaag gccggattat aggaacatca catttgatga gcttgttgaa gcataccaag 601 agcaggccaa aggacttctg gatggcgggg ttgatatctt actcattgaa actatttttg 661 atactgccaa tgccaaggca gccttgtttg cactccaaaa tctttttgag gagaaatatg 721 ctccccggcc tatctttatt tcagggacga tcgttgataa aagtgggcgg actctttccg 781 gacagacagg agagggattt gtcatcagcg tgtctcatgg agaaccactc tgcattggat 841 taaattgtgc tttgggtgca gctgaaatga gaccttttat tgaaataatt ggaaaatgta 901 caacagccta tgtcctctgt tatcccaatg caggtcttcc caacaccttt ggtgactatg 961 atgaaacgcc ttctatgatg gccaagcacc taaaggattt tgctatggat ggcttggtca 1021 atatagttgg aggatgctgt gggtcaacac cagatcatat cagggaaatt gctgaagctg 1081 tgaaaaattg taagcctaga gttccacctg ccactgcttt tgaaggacat atgttactgt 1141 ctggtctaga gcccttcagg attggaccgt acaccaactt tgttaacatt ggagagcgct 1201 gtaatgttgc aggatcaagg aagtttgcta aactcatcat ggcaggaaac tatgaagaag 1261 ccttgtgtgt tgccaaagtg caggtggaaa tgggagccca ggtgttggat gtcaacatgg 1321 atgatggcat gctagatggt ccaagtgcaa tgaccagatt ttgcaactta attgcttccg 1381 agccagacat cgcaaaggta cctttgtgca tcgactcctc caattttgct gtgattgaag 1441 ctgggttaaa gtgctgccaa gggaagtgca ttgtcaatag cattagtctg aaggaaggag 1501 aggacgactt cttggagaag gccaggaaga ttaaaaagta tgcgagctgc tggtgatgct 1561 tttgatgaag aaggacaggc aacagaaaca gacacaaaaa tcagagtgtg cacccgggcc 1621 taccatctgc ttgtgaaaaa actgggcttt aatccaaatg acattatttt tgaccctaat 1681 atcctaacca ttgggactgg aatggaggaa cacaacttgt atgccattaa ttttatccat 1741 gcaacaaaag tcattaaaga aacattacct ggagccagaa taagtggagg tctttccaac 1801 ttgtccttct ccttccgagg aatggaagcc attcgagaag caatgcatgg ggttttcctt 1861 taccatgcaa tcaagtctgg catggacatg gggatagtga atgctggaaa cctccctgtg 1921 tatgatgata tccataagga acttctgcag ctctgtgaag atctcatctg gaataaagac 1981 cctgaggcca ctgagaagct cttacgttat gcccagactc aaggcacagg aggggaatga 2041 aagtcattca gactgatgag tggagaaatg gccctgtcga agaacgcctt gagtatgccc 2101 ttgtgaaggg cattgaaaaa catattattg aggatactga ggaagccagg ttaaaccaaa 2161 aaaaatatcc ccgacctctc aatataattg aaggacccct gatgaatgga atgaaaattg 2221 ttggtgatct ttttggagct ggaaaaatgt ttctacctca ggttataaag tcagcccggg 2281 ttatgaagaa ggctgttggc caccttatcc ctttcatgga aaaagaaaga gaagaaacca 2341 gagtgcttaa cggcacagta gaagaagagg acccttacca gggcaccatc gtgctggcca 2401 ctgttaaagg cgacgtgcac gacataggca agaacatagt tggagtagtc cttggctgca 2461 ataatttccg agttattgat ttaggagtca tgactccatg tgataagata ctgaaagctg 2521 ctcttgacca caaagcagat ataattggcc tgtcaggact catcactcct tccctggatg 2581 aaatgatttt tgttgccaag gaaatggaga gattagctat aaggattcca ttgttgattg 2641 gaggagcaac cacttcaaaa acccacacag cagttaaaat agctccgaga tacagtgcac 2701 ctgtaatcca tgtcctggac gcgtccaaga gtgtggtggt gtgttcccag ctgttagatg 2761 aaaatctaaa ggatgaatac tttgaggaaa tcatggaaga atatgaagat attagacagg 2821 gccattatga gtctctcaag gagaggagat acttaccctt aagtcaagcc agaaaaagtg 2881 gtttccaaat ggattggctg tctgaacctc acccagtgaa gcccacgttt attgggaccc 2941 aggtctttga agactatgac ctgcagaagc tggtggacta cattgactgg aagcctttct 3001 ttgatgtctg gcagctccgg ggcaagtacc cgaatcgagg ctttcccaag atatttaacg 3061 acaaaacagt aggtggagag gccaggaagg tctacgatga tgcccacaat atgctgaaca 3121 cactgattag tcaaaagaaa ctccgggccc ggggtgtggt tgggttctgg ccagcacaga 3181 gtatccaaga cgacattcac ctgtacgcag aggctgctgt gccccaggct gcagagccca 3241 tagccacctt ctatgggtta aggcaacagg ctgagaagga ctctgccagc acggagccat 3301 actactgcct ctcagacttc atcgctccct tgcattctgg catccgtgac tacctgggcc 3361 tgtttgccgt tgcctgcttt ggggtagaag agctgagcaa ggcctatgag gatgatggtg 3421 acgactacag cagcatcatg gtcaaggcgc tgggggaccg gctggcagag gcctttgcag 3481 aagagctcca tgaaagagtt cgccgagaac tgtgggccta ctgtggcagt gagcagctgg 3541 acgtcgcaga cctgcgcagg ctgcggtaca agggcatccg cccggctcct ggctacccca 3601 gccagcccga ccacaccgag aagctcacca tgtggagact tgcagacatc gagcagtcta 3661 caggcattag gttaacagaa tcattagcaa tggcacctgc ttcagcagtc tcaggcctct 3721 acttctccaa tttgaagtcc aaatattttg ctgtggggaa gatttccaag gatcaggttg 3781 aggattatgc attgaggaag aacatatctg tggctgaggt tgagaaatgg cttggaccca 3841 ttttgggata tgatacagac ttgccaactt tcttgtacaa agtggttggt aagcctatcc 3901 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 3961 ttaagtcgac aatcaacctc tggatta