Construct: ORF ccsbBroad304_13904

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015388.1_s304c1, BRDN0000462410
Derived from:
ccsbBroadEn_13904
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MTR (4548)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13904
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4548 MTR 5-methyltetrahydrofolate-ho... NM_000254.2 99.6% 38.8% (many diffs)
2 human 4548 MTR 5-methyltetrahydrofolate-ho... XM_005273141.5 99.5% 38.7% (many diffs)
3 human 4548 MTR 5-methyltetrahydrofolate-ho... NM_001291939.1 95.6% 40.4% (many diffs)
4 human 4548 MTR 5-methyltetrahydrofolate-ho... XM_011544194.3 91.9% 30.1% (many diffs)
5 human 4548 MTR 5-methyltetrahydrofolate-ho... XM_017001329.2 88.1% 31.2% (many diffs)
6 human 4548 MTR 5-methyltetrahydrofolate-ho... XM_017001330.2 87.2% 31.5% (many diffs)
7 human 4548 MTR 5-methyltetrahydrofolate-ho... XM_006711770.3 75% 14.1% (many diffs)
8 human 4548 MTR 5-methyltetrahydrofolate-ho... NM_001291940.2 67.5% 6.6% (many diffs)
9 mouse 238505 Mtr 5-methyltetrahydrofolate-ho... NM_001081128.3 87.3% 34.9% (many diffs)
10 mouse 238505 Mtr 5-methyltetrahydrofolate-ho... XM_006516661.3 69.7% 16.6% (many diffs)
11 mouse 238505 Mtr 5-methyltetrahydrofolate-ho... XM_006516662.3 69.3% 44% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1554
ORF length:
1485
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtcacccgcg ctccaagacc tgtcgcaacc cgaaggtctg aagaaaaccc
121tgcgggatga gatcaatgcc attctgcaga agaggattat ggtgctggat ggagggatgg
181ggaccatgat ccagcgggag aagctaaacg aagaacactt ccgaggtcag gaatttaaag
241atcatgccag gccgctgaaa ggcaacaatg acattttaag tataactcag cctgatgtca
301tttaccaaat ccataaggaa tacttgctgg ctggggcaga tatcattgaa acaaatactt
361ttagcagcac tagtattgcc caagctgact atggccttga acacttggcc taccggatga
421acatgtgctc tgcaggagtg gccagaaaag ctgccgagga ggtaactctc cagacaggaa
481ttaagaggtt tgtggcaggg gctctgggtc cgactaataa gacactctct gtgtccccat
541ctgtggaaag gccggattat aggaacatca catttgatga gcttgttgaa gcataccaag
601agcaggccaa aggacttctg gatggcgggg ttgatatctt actcattgaa actatttttg
661atactgccaa tgccaaggca gccttgtttg cactccaaaa tctttttgag gagaaatatg
721ctccccggcc tatctttatt tcagggacga tcgttgataa aagtgggcgg actctttccg
781gacagacagg agagggattt gtcatcagcg tgtctcatgg agaaccactc tgcattggat
841taaattgtgc tttgggtgca gctgaaatga gaccttttat tgaaataatt ggaaaatgta
901caacagccta tgtcctctgt tatcccaatg caggtcttcc caacaccttt ggtgactatg
961atgaaacgcc ttctatgatg gccaagcacc taaaggattt tgctatggat ggcttggtca
1021atatagttgg aggatgctgt gggtcaacac cagatcatat cagggaaatt gctgaagctg
1081tgaaaaattg taagcctaga gttccacctg ccactgcttt tgaaggacat atgttactgt
1141ctggtctaga gcccttcagg attggaccgt acaccaactt tgttaacatt ggagagcgct
1201gtaatgttgc aggatcaagg aagtttgcta aactcatcat ggcaggaaac tatgaagaag
1261ccttgtgtgt tgccaaagtg caggtggaaa tgggagccca ggtgttggat gtcaacatgg
1321atgatggcat gctagatggt ccaagtgcaa tgaccagatt ttgcaactta attgcttccg
1381agccagacat cgcaaaggta cctttgtgca tcgactcctc caattttgct gtgattgaag
1441ctgggttaaa gtgctgccaa gggaagtgca ttgtcaatag cattagtctg aaggaaggag
1501aggacgactt cttggagaag gccaggaaga ttaaaaagta tgcgagctgc tggtgatgct
1561tttgatgaag aaggacaggc aacagaaaca gacacaaaaa tcagagtgtg cacccgggcc
1621taccatctgc ttgtgaaaaa actgggcttt aatccaaatg acattatttt tgaccctaat
1681atcctaacca ttgggactgg aatggaggaa cacaacttgt atgccattaa ttttatccat
1741gcaacaaaag tcattaaaga aacattacct ggagccagaa taagtggagg tctttccaac
1801ttgtccttct ccttccgagg aatggaagcc attcgagaag caatgcatgg ggttttcctt
1861taccatgcaa tcaagtctgg catggacatg gggatagtga atgctggaaa cctccctgtg
1921tatgatgata tccataagga acttctgcag ctctgtgaag atctcatctg gaataaagac
1981cctgaggcca ctgagaagct cttacgttat gcccagactc aaggcacagg aggggaatga
2041aagtcattca gactgatgag tggagaaatg gccctgtcga agaacgcctt gagtatgccc
2101ttgtgaaggg cattgaaaaa catattattg aggatactga ggaagccagg ttaaaccaaa
2161aaaaatatcc ccgacctctc aatataattg aaggacccct gatgaatgga atgaaaattg
2221ttggtgatct ttttggagct ggaaaaatgt ttctacctca ggttataaag tcagcccggg
2281ttatgaagaa ggctgttggc caccttatcc ctttcatgga aaaagaaaga gaagaaacca
2341gagtgcttaa cggcacagta gaagaagagg acccttacca gggcaccatc gtgctggcca
2401ctgttaaagg cgacgtgcac gacataggca agaacatagt tggagtagtc cttggctgca
2461ataatttccg agttattgat ttaggagtca tgactccatg tgataagata ctgaaagctg
2521ctcttgacca caaagcagat ataattggcc tgtcaggact catcactcct tccctggatg
2581aaatgatttt tgttgccaag gaaatggaga gattagctat aaggattcca ttgttgattg
2641gaggagcaac cacttcaaaa acccacacag cagttaaaat agctccgaga tacagtgcac
2701ctgtaatcca tgtcctggac gcgtccaaga gtgtggtggt gtgttcccag ctgttagatg
2761aaaatctaaa ggatgaatac tttgaggaaa tcatggaaga atatgaagat attagacagg
2821gccattatga gtctctcaag gagaggagat acttaccctt aagtcaagcc agaaaaagtg
2881gtttccaaat ggattggctg tctgaacctc acccagtgaa gcccacgttt attgggaccc
2941aggtctttga agactatgac ctgcagaagc tggtggacta cattgactgg aagcctttct
3001ttgatgtctg gcagctccgg ggcaagtacc cgaatcgagg ctttcccaag atatttaacg
3061acaaaacagt aggtggagag gccaggaagg tctacgatga tgcccacaat atgctgaaca
3121cactgattag tcaaaagaaa ctccgggccc ggggtgtggt tgggttctgg ccagcacaga
3181gtatccaaga cgacattcac ctgtacgcag aggctgctgt gccccaggct gcagagccca
3241tagccacctt ctatgggtta aggcaacagg ctgagaagga ctctgccagc acggagccat
3301actactgcct ctcagacttc atcgctccct tgcattctgg catccgtgac tacctgggcc
3361tgtttgccgt tgcctgcttt ggggtagaag agctgagcaa ggcctatgag gatgatggtg
3421acgactacag cagcatcatg gtcaaggcgc tgggggaccg gctggcagag gcctttgcag
3481aagagctcca tgaaagagtt cgccgagaac tgtgggccta ctgtggcagt gagcagctgg
3541acgtcgcaga cctgcgcagg ctgcggtaca agggcatccg cccggctcct ggctacccca
3601gccagcccga ccacaccgag aagctcacca tgtggagact tgcagacatc gagcagtcta
3661caggcattag gttaacagaa tcattagcaa tggcacctgc ttcagcagtc tcaggcctct
3721acttctccaa tttgaagtcc aaatattttg ctgtggggaa gatttccaag gatcaggttg
3781aggattatgc attgaggaag aacatatctg tggctgaggt tgagaaatgg cttggaccca
3841ttttgggata tgatacagac ttgccaactt tcttgtacaa agtggttggt aagcctatcc
3901ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
3961ttaagtcgac aatcaacctc tggatta

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