Transcript: Mouse XM_006517412.3

PREDICTED: Mus musculus Rho-related BTB domain containing 3 (Rhobtb3), transcript variant X5, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Rhobtb3 (73296)
Length:
4377
CDS:
315..1568

Additional Resources:

NCBI RefSeq record:
XM_006517412.3
NBCI Gene record:
Rhobtb3 (73296)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006517412.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000241351 CGCCACTAACTACCTCATATT pLKO_005 1400 CDS 100% 13.200 18.480 N Rhobtb3 n/a
2 TRCN0000241347 TCCCGGAAATGTCGCTGTTTA pLKO_005 1539 CDS 100% 13.200 18.480 N Rhobtb3 n/a
3 TRCN0000048645 GCTTCATTTATTCAGGTGCTT pLKO.1 766 CDS 100% 2.640 3.696 N RHOBTB3 n/a
4 TRCN0000241349 ACTGAGTAGCCTGACTATAAT pLKO_005 2731 3UTR 100% 15.000 10.500 N Rhobtb3 n/a
5 TRCN0000216115 CAAGAACTGATAGACTATTTG pLKO.1 2877 3UTR 100% 13.200 9.240 N Rhobtb3 n/a
6 TRCN0000241350 CAGACATTCTGCGCTTCATTT pLKO_005 754 CDS 100% 13.200 9.240 N Rhobtb3 n/a
7 TRCN0000184301 GCTGGCAGAATACAGGAAGTA pLKO.1 1511 CDS 100% 4.950 2.970 N Rhobtb3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006517412.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07806 pDONR223 100% 60.7% 64.3% None (many diffs) n/a
2 ccsbBroad304_07806 pLX_304 0% 60.7% 64.3% V5 (many diffs) n/a
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