Construct: ORF ccsbBroad304_07806
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013486.1_s304c1, BRDN0000403838
- Derived from:
- ccsbBroadEn_07806
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHOBTB3 (22836)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07806
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22836 | RHOBTB3 | Rho related BTB domain cont... | NM_014899.4 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 22836 | RHOBTB3 | Rho related BTB domain cont... | XM_011543279.2 | 79.9% | 79.8% | 59G>A;510G>A;679_680ins366 |
| 3 | human | 22836 | RHOBTB3 | Rho related BTB domain cont... | XM_017009236.1 | 68.1% | 68% | 0_1ins582;195C>T;202A>G |
| 4 | human | 22836 | RHOBTB3 | Rho related BTB domain cont... | XM_017009237.1 | 68.1% | 68% | 0_1ins582;195C>T;202A>G |
| 5 | mouse | 73296 | Rhobtb3 | Rho-related BTB domain cont... | NM_028493.2 | 89.7% | 95.5% | (many diffs) |
| 6 | mouse | 73296 | Rhobtb3 | Rho-related BTB domain cont... | XM_006517410.3 | 89.6% | 95.4% | (many diffs) |
| 7 | mouse | 73296 | Rhobtb3 | Rho-related BTB domain cont... | XM_006517411.3 | 81.2% | 86.2% | (many diffs) |
| 8 | mouse | 73296 | Rhobtb3 | Rho-related BTB domain cont... | XM_011244573.2 | 73.5% | 74.6% | (many diffs) |
| 9 | mouse | 73296 | Rhobtb3 | Rho-related BTB domain cont... | XM_011244574.2 | 69.3% | 73.1% | (many diffs) |
| 10 | mouse | 73296 | Rhobtb3 | Rho-related BTB domain cont... | XM_006517412.3 | 60.7% | 64.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1899
- ORF length:
- 1833
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc catccacatc gtggcgctgg ggaacgaggg ggacacattc caccaggaca 121 accagccgtc ggggcttatc cgcacttacc tggggagaag ccctctggtc tccggggacg 181 agagcagctt gttgctgaac gcggccagca cggtcgcgcg tccggtgttc accgagtatc 241 aggccagtgc gtttgggaat gtcaagctgg tggtccacga ctgtcccgtc tgggacatat 301 ttgacagtga ttggtacact tctcgaaatc taattggggg cgctgacatc attgtgatca 361 aatacaacgt taatgacaag ttttcattcc atgaagtaaa ggataattat attccagtga 421 taaaaagagc attaaattca gttccagtaa ttattgctgc tgttggtacc agacaaaatg 481 aagagttacc ttgtacatgc ccactatgta cctcagacag agggagctgt gttagtacaa 541 ctgaagggat ccaacttgca aaagaactag gagcaaccta tcttgaactc cacagccttg 601 atgacttcta cataggaaag tattttggag gagtgttgga gtattttatg attcaagcct 661 taaatcagaa gacaagtgaa aaaatgaaga aaagaaaaat gagcaactcc tttcatggaa 721 ttagaccacc tcaacttgaa caaccagaaa aaatgcctgt cttaaaggct gaagcgtcac 781 attataactc tgacttaaat aacttgctgt tctgctgcca gtgtgtggac gtggtatttt 841 ataaccccga tttaaagaaa gttgtagagg cccacaagat cgttctctgc gctgtaagcc 901 atgttttcat gctgcttttc aatgtgaaga gtcccactga cattcaggat tccagtatca 961 tccgaactac ccaggatctt tttgctataa acagagatac tgcatttcca ggtgctagcc 1021 atgaatcttc aggcaaccca ccattacgag tcattgttaa agacgccctc ttctgttctt 1081 gtttatcaga catccttcgc ttcatttatt caggtgcttt tcagtgggaa gaattggaag 1141 aagatatcag gaagaagttg aaagattctg gggatgtttc aaatgtaatc gagaaagtta 1201 aatgcatttt aaaaacacca ggaaagatta attgcctaag gaattgcaaa acctatcaag 1261 ccagaaaacc tttgtggttt tataacactt ccctcaagtt tttccttaat aagccgatgc 1321 ttgccgatgt tgtcttcgaa attcaaggta cgacagtgcc agcccacagg gccatcctgg 1381 tggcccgttg tgaagtgatg gcagccatgt ttaatggtaa ttacatggaa gcaaagagtg 1441 tcctgattcc cgtttatggt gtttccaaag agactttctt gtcattttta gaatacctgt 1501 acacagactc ctgctgccca gctggcatat tccaggccat gtgtctcctg atctgtgccg 1561 agatgtacca agtgtccaga ctgcagcaca tctgtgagct gttcatcatt acccagctgc 1621 agagcatgcc aagcagggaa ctggcatcca tgaaccttga tatagttgac ctgcttaaaa 1681 aggccaagtt tcaccactct gattgccttt caacctggct acttcatttc attgctacta 1741 actacctcat cttcagtcaa aagcctgaat ttcaggatct ttcagtggaa gaacgcagtt 1801 ttgttgaaaa gcacagatgg ccgtcgaata tgtacttgaa gcagcttgcg gaatacagga 1861 agtatattca ctcccggaaa tgtcgttgct tagtaatgta cccaactttc ttgtacaaag 1921 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1981 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta