Transcript: Mouse XM_006520143.1

PREDICTED: Mus musculus golgi phosphoprotein 3 (Golph3), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Golph3 (66629)
Length:
3109
CDS:
536..1300

Additional Resources:

NCBI RefSeq record:
XM_006520143.1
NBCI Gene record:
Golph3 (66629)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006520143.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000111624 GCAGTATGATCTAGCCACCAA pLKO.1 1180 CDS 100% 2.640 3.696 N Golph3 n/a
2 TRCN0000351297 GCAGTATGATCTAGCCACCAA pLKO_005 1180 CDS 100% 2.640 3.696 N Golph3 n/a
3 TRCN0000111622 GCTGTTCTTGACAAATGGGTA pLKO.1 1061 CDS 100% 2.640 3.696 N Golph3 n/a
4 TRCN0000309864 GCTGTTCTTGACAAATGGGTA pLKO_005 1061 CDS 100% 2.640 3.696 N Golph3 n/a
5 TRCN0000111620 CCCACACTAATAATTGGCTTT pLKO.1 1385 3UTR 100% 4.050 2.835 N Golph3 n/a
6 TRCN0000351399 CCCACACTAATAATTGGCTTT pLKO_005 1385 3UTR 100% 4.050 2.835 N Golph3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006520143.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03918 pDONR223 100% 78.8% 83.8% None (many diffs) n/a
2 ccsbBroad304_03918 pLX_304 0% 78.8% 83.8% V5 (many diffs) n/a
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