Construct: ORF ccsbBroad304_03918
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000639.1_s304c1, BRDN0000405081
- Derived from:
- ccsbBroadEn_03918
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLPH3 (64083)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03918
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64083 | GOLPH3 | golgi phosphoprotein 3 | NM_022130.4 | 100% | 100% | |
| 2 | human | 64083 | GOLPH3 | golgi phosphoprotein 3 | XM_017009686.2 | 69.1% | 69.1% | 0_1ins276 |
| 3 | mouse | 66629 | Golph3 | golgi phosphoprotein 3 | NM_025673.2 | 92.8% | 98.6% | (many diffs) |
| 4 | mouse | 66629 | Golph3 | golgi phosphoprotein 3 | XM_006520143.1 | 78.8% | 83.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac ctcgctgacc cagcgcagct ccggcctggt gcagcggcgc accgaggcct 121 cccgcaacgc cgccgacaag gagcgggcgg cgggcggcgg cgccggcagc agcgaggacg 181 acgcgcagag ccgccgcgac gagcaggacg acgacgacaa gggcgactcc aaggaaacgc 241 ggctgaccct gatggaggaa gtgctcctgc tgggcctcaa ggaccgcgag ggttacacat 301 cattttggaa tgactgtata tcatctggat tacgtggctg tatgttaatt gaattagcat 361 tgagaggaag gttacaacta gaggcttgtg gaatgagacg taaaagtcta ttaacaagaa 421 aggtaatctg taagtcagat gctccaacag gggatgttct tcttgatgaa gctctgaagc 481 atgttaagga aactcagcct ccagaaacgg tccagaactg gattgaatta cttagtggtg 541 agacatggaa tccattaaaa ttgcattatc agttaagaaa tgtacgggaa cgattagcta 601 aaaacctggt ggaaaagggt gtattgacaa cagagaaaca gaacttccta ctttttgaca 661 tgacaacaca tcccctcacc aataacaaca ttaagcagcg cctcatcaag aaagtacagg 721 aagccgttct tgacaaatgg gtgaatgacc ctcaccgcat ggacaggcgc ttgctggccc 781 tcatttacct ggctcatgcc tcggacgtcc tggagaatgc ttttgctcct cttctggacg 841 agcagtatga tttggctacc aagagagtgc ggcagcttct cgacttagac cctgaagtgg 901 aatgtctgaa ggccaacacc aatgaggttc tgtgggcggt ggtggcggcg ttcaccaagt 961 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1021 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1081 ggatta