Transcript: Mouse XM_006520246.3

PREDICTED: Mus musculus small G protein signaling modulator 3 (Sgsm3), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Sgsm3 (105835)
Length:
2843
CDS:
219..2504

Additional Resources:

NCBI RefSeq record:
XM_006520246.3
NBCI Gene record:
Sgsm3 (105835)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006520246.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000106024 CCCTGTTTGAACACGGATTGA pLKO.1 1960 CDS 100% 4.950 6.930 N Sgsm3 n/a
2 TRCN0000106022 GCCTCGCTTAGACAAACTGTT pLKO.1 1028 CDS 100% 4.950 3.960 N Sgsm3 n/a
3 TRCN0000106023 CCTGGTGCTCTGTAAGACATA pLKO.1 2081 CDS 100% 4.950 3.465 N Sgsm3 n/a
4 TRCN0000106020 CCCATCATAGACTGCCTGGAA pLKO.1 2540 3UTR 100% 2.640 1.848 N Sgsm3 n/a
5 TRCN0000106021 GCCTACCTTATTGCAGACCAA pLKO.1 1374 CDS 100% 2.640 1.848 N Sgsm3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006520246.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03039 pDONR223 100% 87.3% 92.6% None (many diffs) n/a
2 ccsbBroad304_03039 pLX_304 0% 87.3% 92.6% V5 (many diffs) n/a
3 TRCN0000470240 AATCAGGGATAGATAGATAACCTT pLX_317 22.5% 87.3% 92.6% V5 (many diffs) n/a
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