Construct: ORF TRCN0000470240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009877.1_s317c1
- Derived from:
- ccsbBroadEn_03039
- DNA Barcode:
- AATCAGGGATAGATAGATAACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SGSM3 (27352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470240
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 27352 | SGSM3 | small G protein signaling m... | NM_015705.6 | 100% | 100% | |
2 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350039.2 | 97.7% | 97.7% | 1989_2039del |
3 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350041.2 | 97.7% | 96.5% | (many diffs) |
4 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452223.1 | 96.3% | 96.3% | 1368_1451del |
5 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350040.2 | 95.6% | 94.4% | (many diffs) |
6 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452222.1 | 94.5% | 94.5% | 1240_1368del |
7 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452221.1 | 94.3% | 94.3% | 1368_1451del;2073_2123del |
8 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350044.2 | 91.5% | 90.3% | 0_1ins127;28_29ins62 |
9 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350045.2 | 91.5% | 90.3% | 0_1ins127;28_29ins62 |
10 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452219.1 | 91.3% | 91.3% | 1240_1368del;1497_1580del |
11 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350046.2 | 91% | 91% | 0_1ins201 |
12 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452227.1 | 90.2% | 90.2% | 473_474ins144;1224_1307del |
13 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350042.2 | 89.5% | 88.3% | 0_1ins127;28_29ins62;1800_1850del |
14 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452220.1 | 89.3% | 88.2% | (many diffs) |
15 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350043.2 | 89% | 89% | 0_1ins201;1788_1838del |
16 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350047.2 | 88.8% | 87.6% | (many diffs) |
17 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452224.1 | 88.2% | 88.2% | 473_474ins144;1224_1307del;1929_1979del |
18 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001301849.2 | 88.1% | 86.9% | 0_1ins127;28_29ins62;1438_1439ins78 |
19 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452229.1 | 87.7% | 87.7% | 0_1ins201;1167_1250del |
20 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452225.1 | 86.3% | 85.2% | (many diffs) |
21 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452226.1 | 86.3% | 85.2% | (many diffs) |
22 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452228.1 | 85.8% | 85.8% | 0_1ins201;1167_1250del;1872_1922del |
23 | human | 27352 | SGSM3 | small G protein signaling m... | XM_017028778.1 | 82.7% | 82.7% | 0_1ins201;272_273ins144;1644_1694del |
24 | human | 27352 | SGSM3 | small G protein signaling m... | XR_001755218.1 | 80.4% | (many diffs) | |
25 | human | 27352 | SGSM3 | small G protein signaling m... | XR_937842.3 | 80.4% | 1_165del;1690_1714del;2438_2792del | |
26 | human | 27352 | SGSM3 | small G protein signaling m... | XR_001755224.1 | 80.1% | (many diffs) | |
27 | human | 27352 | SGSM3 | small G protein signaling m... | XR_001755222.2 | 79.9% | (many diffs) | |
28 | human | 27352 | SGSM3 | small G protein signaling m... | XR_001755220.2 | 79% | (many diffs) | |
29 | human | 27352 | SGSM3 | small G protein signaling m... | XR_002958687.1 | 78.2% | (many diffs) | |
30 | human | 27352 | SGSM3 | small G protein signaling m... | NM_001350048.2 | 77.8% | 77.8% | 0_1ins498 |
31 | human | 27352 | SGSM3 | small G protein signaling m... | NR_146412.2 | 74.7% | 1_206del;2056_2075del;2474_3006del | |
32 | human | 27352 | SGSM3 | small G protein signaling m... | XR_937841.1 | 74.5% | (many diffs) | |
33 | human | 27352 | SGSM3 | small G protein signaling m... | NR_146415.2 | 74.4% | (many diffs) | |
34 | human | 27352 | SGSM3 | small G protein signaling m... | NR_146413.2 | 74.3% | (many diffs) | |
35 | human | 27352 | SGSM3 | small G protein signaling m... | XR_001755223.1 | 73.9% | (many diffs) | |
36 | human | 27352 | SGSM3 | small G protein signaling m... | XR_001755221.2 | 73.8% | (many diffs) | |
37 | human | 27352 | SGSM3 | small G protein signaling m... | XR_002958688.1 | 73.7% | (many diffs) | |
38 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452230.1 | 73.4% | 73.4% | 0_1ins498;870_953del;1575_1625del |
39 | human | 27352 | SGSM3 | small G protein signaling m... | XM_024452231.1 | 73.4% | 73.4% | 0_1ins498;870_953del;1575_1625del |
40 | human | 27352 | SGSM3 | small G protein signaling m... | XR_001755225.2 | 73.3% | (many diffs) | |
41 | human | 27352 | SGSM3 | small G protein signaling m... | XR_002958686.1 | 72.4% | (many diffs) | |
42 | human | 27352 | SGSM3 | small G protein signaling m... | NR_146414.2 | 71.5% | (many diffs) | |
43 | human | 27352 | SGSM3 | small G protein signaling m... | XR_001755217.1 | 71.2% | (many diffs) | |
44 | mouse | 105835 | Sgsm3 | small G protein signaling m... | NM_134091.2 | 88.3% | 93.7% | (many diffs) |
45 | mouse | 105835 | Sgsm3 | small G protein signaling m... | XM_006520244.3 | 87.3% | 92.6% | (many diffs) |
46 | mouse | 105835 | Sgsm3 | small G protein signaling m... | XM_006520245.3 | 87.3% | 92.6% | (many diffs) |
47 | mouse | 105835 | Sgsm3 | small G protein signaling m... | XM_006520246.3 | 87.3% | 92.6% | (many diffs) |
48 | mouse | 105835 | Sgsm3 | small G protein signaling m... | XM_006520247.3 | 87.1% | 92.5% | (many diffs) |
49 | mouse | 105835 | Sgsm3 | small G protein signaling m... | XM_006520248.3 | 87.1% | 92.5% | (many diffs) |
50 | mouse | 105835 | Sgsm3 | small G protein signaling m... | XM_006520249.3 | 85% | 90% | (many diffs) |
51 | mouse | 105835 | Sgsm3 | small G protein signaling m... | XM_017316358.1 | 69.1% | 74.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2313
- ORF length:
- 2247
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aggaagccat acacctgcct gtggcccttt ctcagccctg actccgagca 121 tatggcccca ggagatcttg gccaagtaca cgcagaagga agagtcagca gagcaaccag 181 agttctacta cgatgagttt ggtttccgtg tgtacaagga agaaggtgat gagcctggct 241 ccagtctgct ggcgaactcc cctctgatgg aggatgctcc acagaggctg cggtggcagg 301 cccacctgga gttcacccat aaccacgatg tgggggatct cacctgggac aagattgccg 361 tctccctacc ccgctctgag aagctccgct ccctggtgct ggccggcatc ccacatggca 421 tgaggccaca gctgtggatg cggctctctg gggccctgca gaagaagagg aactctgagc 481 tgtcctaccg cgagattgtg aagaacagct ccaacgatga gaccatcgct gccaagcaga 541 tcgagaagga cctgctccgc accatgccca gcaacgcctg cttcgccagc atgggtagca 601 tcggggtgcc ccgcctgcgc agggtgctcc gggccctggc ctggctctac ccagagatcg 661 gctactgcca gggcaccggc atggtggccg cctgcctcct gctgttcctg gaggaggagg 721 acgccttctg gatgatgtct gccatcatcg aggacctgct ccccgcctcc tacttcagca 781 ccaccctgct gggtgtccag actgaccagc gggtcctgcg ccacctcatt gtccagtacc 841 tgcctcgcct ggacaagctg ctccaggagc atgacattga gctgtccctg atcacactgc 901 actggttcct cacggccttc gccagcgtgg tggacatcaa gctgctcctg cgcatctggg 961 acctgttttt ctacgagggc tcccgggtgc tgttccagct cacgctgggc atgctgcacc 1021 tcaaggagga agagctgatc cagtcagaga actcggcctc catcttcaac acgctatcgg 1081 atatcccgtc gcagatggag gacgcggagc tgcttctggg ggtggccatg cggctggccg 1141 gctccctcac cgatgtggcc gtggagactc agcgccgcaa gcacctggcc tatctcattg 1201 cagaccaggg ccagctcctg ggggccggca ccctcaccaa cctctctcag gttgttcgcc 1261 gcaggaccca gcggaggaag tccaccatca ctgctctgct cttcggggag gatgacctgg 1321 aggcactcaa ggccaagaac atcaagcaga cggaactggt ggctgacctc cgggaagcca 1381 tcctgcgcgt ggcacgccac ttccagtgca cagaccccaa aaactgcagc gtggagctga 1441 ctccagacta tagcatggag agccaccagc gggaccacga gaactacgtg gcgtgctcac 1501 gcagccaccg gcgccgagcc aaggccctgc tggactttga gcggcacgac gacgacgagc 1561 tgggcttccg caagaacgac atcatcacaa tcgtgtctca gaaggacgag cactgctggg 1621 tgggggagct caacggcctg cgaggctggt ttccagccaa gttcgtggaa gtcctggatg 1681 agcgcagcaa agagtactcc atcgcggggg atgactcggt gacggagggg gtcacagacc 1741 tcgtgcgagg gaccctctgc ccggccctta aggccctgtt cgaacatgga ctgaagaagc 1801 catccctgct tGGGGGCGCC TGCCACCCCT GGCTGTTTAT CGAGGAGGCT GCAGGCCGGG 1861 AGGTCGAGAG AGACTTTGCC TCCGTGTATT CCCGTCTGGT GCTCTGTAAG ACCTTCAGGT 1921 TGGATGAAGA TGGCAAAGTC CTGACCCCGG AGGAGCTGCT CTACCGGGCT GTGCAGTCTG 1981 TGAACGTGAC CCACGATGCA GTGCATGCAC AAATGGATGT GAAGCTCCGC TCACTGATCT 2041 GCGTGGGGCT CAATGAGCAG GTGCTGCACC TGTGGCTGGA GGTGCTCTGC TCCAGCCTGC 2101 CCACCGTGGA GAAGTGGTAC CAGCCCTGGT CCTTCCTGCG CAGCCCGGGC TGGGTCCAGA 2161 TCAAGTGTGA GCTCCGAGTC CTCTGCTGCT TTGCCTTCAG CCTCTCCCAG GACTGGGAGC 2221 TCCCTGCGAA GAGAGAGGCG CAGCAGCCCC TGAAGGAGGG CGTCCGGGAC ATGCTGGTGA 2281 AGCACCACCT CTTCAGCTGG GATGTGGACG GGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 2341 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2401 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA 2461 TCAGGGATAG ATAGATAACC TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 2521 ttgtgaaaga tt