Transcript: Mouse XM_006522109.3

PREDICTED: Mus musculus MKL/myocardin-like 2 (Mkl2), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mkl2 (239719)
Length:
8548
CDS:
376..3714

Additional Resources:

NCBI RefSeq record:
XM_006522109.3
NBCI Gene record:
Mkl2 (239719)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006522109.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000085861 CCCAAGAATCCAAACGACAAA pLKO.1 1279 CDS 100% 4.950 6.930 N Mkl2 n/a
2 TRCN0000085860 CCTCATAAAGAGTGGAGAGAT pLKO.1 3162 CDS 100% 4.950 3.960 N Mkl2 n/a
3 TRCN0000085858 GCCAGTCACAACACTGCATAA pLKO.1 1815 CDS 100% 10.800 7.560 N Mkl2 n/a
4 TRCN0000085859 GCAGTGAAGATAGAGAGTCTT pLKO.1 3419 CDS 100% 4.950 3.465 N Mkl2 n/a
5 TRCN0000183992 CACTTACCCACTCTGAACGAA pLKO.1 499 CDS 100% 3.000 2.100 N Mkl2 n/a
6 TRCN0000085862 CCTTTAATACATCAGATCCTA pLKO.1 2288 CDS 100% 3.000 2.100 N Mkl2 n/a
7 TRCN0000090508 GCTGGCCTCAAACTCAGAAAT pLKO.1 7322 3UTR 100% 13.200 6.600 Y Dync1li1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006522109.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12353 pDONR223 100% 28.5% 25.4% None (many diffs) n/a
2 ccsbBroad304_12353 pLX_304 0% 28.5% 25.4% V5 (many diffs) n/a
3 TRCN0000467244 CGATCCCTGGTATGATTTCTCTGG pLX_317 31.4% 28.5% 25.4% V5 (many diffs) n/a
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