Construct: ORF TRCN0000467244
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007372.1_s317c1
- Derived from:
- ccsbBroadEn_12353
- DNA Barcode:
- CGATCCCTGGTATGATTTCTCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRTFB (57496)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467244
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57496 | MRTFB | myocardin related transcrip... | NM_001365416.1 | 79.2% | 76.9% | (many diffs) |
| 2 | human | 57496 | MRTFB | myocardin related transcrip... | NM_001365415.1 | 76.4% | 74.1% | (many diffs) |
| 3 | human | 57496 | MRTFB | myocardin related transcrip... | XM_017023505.2 | 33.6% | 30.4% | (many diffs) |
| 4 | human | 57496 | MRTFB | myocardin related transcrip... | XM_017023504.1 | 33.1% | 29.9% | (many diffs) |
| 5 | human | 57496 | MRTFB | myocardin related transcrip... | NM_014048.4 | 32.7% | 29.6% | (many diffs) |
| 6 | human | 57496 | MRTFB | myocardin related transcrip... | XM_006720914.2 | 32.7% | 29.6% | (many diffs) |
| 7 | human | 57496 | MRTFB | myocardin related transcrip... | NM_001365412.2 | 32.6% | 29.4% | (many diffs) |
| 8 | human | 57496 | MRTFB | myocardin related transcrip... | NM_001365414.2 | 32.4% | 29.2% | (many diffs) |
| 9 | human | 57496 | MRTFB | myocardin related transcrip... | XM_017023503.2 | 32.3% | 29.2% | (many diffs) |
| 10 | human | 57496 | MRTFB | myocardin related transcrip... | XM_006720912.4 | 32.1% | 29% | (many diffs) |
| 11 | human | 57496 | MRTFB | myocardin related transcrip... | NM_001365411.2 | 31.6% | 28.5% | (many diffs) |
| 12 | human | 57496 | MRTFB | myocardin related transcrip... | NM_001308142.2 | 31.3% | 28.2% | (many diffs) |
| 13 | human | 57496 | MRTFB | myocardin related transcrip... | XM_005255452.3 | 31.3% | 28.2% | (many diffs) |
| 14 | human | 57496 | MRTFB | myocardin related transcrip... | XM_005255453.5 | 31.3% | 28.2% | (many diffs) |
| 15 | human | 57496 | MRTFB | myocardin related transcrip... | XM_006720908.4 | 31.3% | 28.2% | (many diffs) |
| 16 | human | 57496 | MRTFB | myocardin related transcrip... | XM_006720909.4 | 31.3% | 28.2% | (many diffs) |
| 17 | human | 57496 | MRTFB | myocardin related transcrip... | XM_011522568.2 | 31.3% | 28.2% | (many diffs) |
| 18 | human | 57496 | MRTFB | myocardin related transcrip... | XM_017023501.2 | 31.3% | 28.2% | (many diffs) |
| 19 | human | 57496 | MRTFB | myocardin related transcrip... | XM_017023502.1 | 31.3% | 28.2% | (many diffs) |
| 20 | human | 57496 | MRTFB | myocardin related transcrip... | NM_001365413.2 | 30.9% | 27.8% | (many diffs) |
| 21 | human | 57496 | MRTFB | myocardin related transcrip... | XM_011522569.2 | 30.9% | 27.8% | (many diffs) |
| 22 | mouse | 239719 | Mkl2 | MKL/myocardin-like 2 | NM_153588.3 | 29.3% | 26.1% | (many diffs) |
| 23 | mouse | 239719 | Mkl2 | MKL/myocardin-like 2 | NM_001122667.2 | 29% | 25.9% | (many diffs) |
| 24 | mouse | 239719 | Mkl2 | MKL/myocardin-like 2 | XM_006522108.2 | 28.5% | 25.4% | (many diffs) |
| 25 | mouse | 239719 | Mkl2 | MKL/myocardin-like 2 | XM_006522109.3 | 28.5% | 25.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1200
- ORF length:
- 1134
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ccagctgagg ctgcaacaaa ggaggacgag agaacaacta gtggaccagg 121 gcatcatgcc acctttgaag agcccagcgg cattccatga acagataaaa agcttggaac 181 gagccagaac tgaaaacttt ttgaaacaca agattcggag tcgaccagat cgttctgaac 241 ttgtcaggat gcacatttta gaagaaacat ttgcagagcc atccctgcag gctactcaga 301 tgaagttgaa aagagctcga ctagcagatg atctgaatga aaagattgct caaagacctg 361 gtcctatgga gctggtagag aaaaacatcc ttcctgtgga ctccagtgtt aaagaagcaa 421 ttataggcgt tgggaaggag gactatcccc acactcaggg cgatttctca tttgatgaag 481 acagcagtga cgctttgtct ccggaccagc ctgcgagtca ggagtcacag gggtcagccg 541 cgtccccaag tgagccaaaa gttagtgaat cgccatctcc tgtgactaca aacactccag 601 cgcagtttgc ttcagtgtcc ccaacagttc ctgaattctt gaaaactcct ccaactgcag 661 atcagcctcc cccacggcct gcagctcctg tcctccccac aaacactgtg tcctcagcaa 721 agcctggccc agcactggtg aagcaaagcc atcccaagaa tccaaatgac aaacaccgta 781 gcaaaaagtg caaagatccc aaaccacggg taaagaagtt aaagtaccac caatacattc 841 caccagaTCA GAAGGGTGAG AAGAATGAGC CGCAGATGGA CTCTAACTAC GCCCGCCTGC 901 TCCAGCAGCA GCAGCTGTTC CTGCAACTGC AGATCCTGAG TCAGCAGAAG CAGCACTACA 961 ACTACCAGAC CATCCTGCCT GCACCATTCA AAGCTTATCA CACAGTGTCT GAAGTCCACA 1021 TGGTGAGAGT GGCCTGCATT CCATTCCAGT TTCTGTCCTC CAAAATAGGT TCTGAATTTT 1081 TACAAGTTAG AAATGCCTTT TCACAATTAT TCATCCAGAT TTGCCTATTA TTAGAACACC 1141 AAAATAGTAC TAGATGCTCT GAGAAAAGCG TAAGTAGTAT AATACCTGGC ATAAACAGCT 1201 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1261 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1321 TTTATATATC TTGTGGAAAG GACGACGATC CCTGGTATGA TTTCTCTGGA CGCGTTAAGT 1381 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt