Construct: ORF TRCN0000467244

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007372.1_s317c1
Derived from:
ccsbBroadEn_12353
DNA Barcode:
CGATCCCTGGTATGATTTCTCTGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MRTFB (57496)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467244
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57496 MRTFB myocardin related transcrip... NM_001365416.1 79.2% 76.9% (many diffs)
2 human 57496 MRTFB myocardin related transcrip... NM_001365415.1 76.4% 74.1% (many diffs)
3 human 57496 MRTFB myocardin related transcrip... XM_017023505.2 33.6% 30.4% (many diffs)
4 human 57496 MRTFB myocardin related transcrip... XM_017023504.1 33.1% 29.9% (many diffs)
5 human 57496 MRTFB myocardin related transcrip... NM_014048.4 32.7% 29.6% (many diffs)
6 human 57496 MRTFB myocardin related transcrip... XM_006720914.2 32.7% 29.6% (many diffs)
7 human 57496 MRTFB myocardin related transcrip... NM_001365412.2 32.6% 29.4% (many diffs)
8 human 57496 MRTFB myocardin related transcrip... NM_001365414.2 32.4% 29.2% (many diffs)
9 human 57496 MRTFB myocardin related transcrip... XM_017023503.2 32.3% 29.2% (many diffs)
10 human 57496 MRTFB myocardin related transcrip... XM_006720912.4 32.1% 29% (many diffs)
11 human 57496 MRTFB myocardin related transcrip... NM_001365411.2 31.6% 28.5% (many diffs)
12 human 57496 MRTFB myocardin related transcrip... NM_001308142.2 31.3% 28.2% (many diffs)
13 human 57496 MRTFB myocardin related transcrip... XM_005255452.3 31.3% 28.2% (many diffs)
14 human 57496 MRTFB myocardin related transcrip... XM_005255453.5 31.3% 28.2% (many diffs)
15 human 57496 MRTFB myocardin related transcrip... XM_006720908.4 31.3% 28.2% (many diffs)
16 human 57496 MRTFB myocardin related transcrip... XM_006720909.4 31.3% 28.2% (many diffs)
17 human 57496 MRTFB myocardin related transcrip... XM_011522568.2 31.3% 28.2% (many diffs)
18 human 57496 MRTFB myocardin related transcrip... XM_017023501.2 31.3% 28.2% (many diffs)
19 human 57496 MRTFB myocardin related transcrip... XM_017023502.1 31.3% 28.2% (many diffs)
20 human 57496 MRTFB myocardin related transcrip... NM_001365413.2 30.9% 27.8% (many diffs)
21 human 57496 MRTFB myocardin related transcrip... XM_011522569.2 30.9% 27.8% (many diffs)
22 mouse 239719 Mkl2 MKL/myocardin-like 2 NM_153588.3 29.3% 26.1% (many diffs)
23 mouse 239719 Mkl2 MKL/myocardin-like 2 NM_001122667.2 29% 25.9% (many diffs)
24 mouse 239719 Mkl2 MKL/myocardin-like 2 XM_006522108.2 28.5% 25.4% (many diffs)
25 mouse 239719 Mkl2 MKL/myocardin-like 2 XM_006522109.3 28.5% 25.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1200
ORF length:
1134
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct ccagctgagg ctgcaacaaa ggaggacgag agaacaacta gtggaccagg
121gcatcatgcc acctttgaag agcccagcgg cattccatga acagataaaa agcttggaac
181gagccagaac tgaaaacttt ttgaaacaca agattcggag tcgaccagat cgttctgaac
241ttgtcaggat gcacatttta gaagaaacat ttgcagagcc atccctgcag gctactcaga
301tgaagttgaa aagagctcga ctagcagatg atctgaatga aaagattgct caaagacctg
361gtcctatgga gctggtagag aaaaacatcc ttcctgtgga ctccagtgtt aaagaagcaa
421ttataggcgt tgggaaggag gactatcccc acactcaggg cgatttctca tttgatgaag
481acagcagtga cgctttgtct ccggaccagc ctgcgagtca ggagtcacag gggtcagccg
541cgtccccaag tgagccaaaa gttagtgaat cgccatctcc tgtgactaca aacactccag
601cgcagtttgc ttcagtgtcc ccaacagttc ctgaattctt gaaaactcct ccaactgcag
661atcagcctcc cccacggcct gcagctcctg tcctccccac aaacactgtg tcctcagcaa
721agcctggccc agcactggtg aagcaaagcc atcccaagaa tccaaatgac aaacaccgta
781gcaaaaagtg caaagatccc aaaccacggg taaagaagtt aaagtaccac caatacattc
841caccagaTCA GAAGGGTGAG AAGAATGAGC CGCAGATGGA CTCTAACTAC GCCCGCCTGC
901TCCAGCAGCA GCAGCTGTTC CTGCAACTGC AGATCCTGAG TCAGCAGAAG CAGCACTACA
961ACTACCAGAC CATCCTGCCT GCACCATTCA AAGCTTATCA CACAGTGTCT GAAGTCCACA
1021TGGTGAGAGT GGCCTGCATT CCATTCCAGT TTCTGTCCTC CAAAATAGGT TCTGAATTTT
1081TACAAGTTAG AAATGCCTTT TCACAATTAT TCATCCAGAT TTGCCTATTA TTAGAACACC
1141AAAATAGTAC TAGATGCTCT GAGAAAAGCG TAAGTAGTAT AATACCTGGC ATAAACAGCT
1201ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1261GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1321TTTATATATC TTGTGGAAAG GACGACGATC CCTGGTATGA TTTCTCTGGA CGCGTTAAGT
1381Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)