Transcript: Mouse XM_006524823.2

PREDICTED: Mus musculus endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1 (Ergic1), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ergic1 (67458)
Length:
2693
CDS:
16..933

Additional Resources:

NCBI RefSeq record:
XM_006524823.2
NBCI Gene record:
Ergic1 (67458)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006524823.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000247352 TGACACATACCATCCATAAAC pLKO_005 497 CDS 100% 13.200 18.480 N Ergic1 n/a
2 TRCN0000175398 CATGACACATACCATCCATAA pLKO.1 495 CDS 100% 10.800 15.120 N Ergic1 n/a
3 TRCN0000247353 CAACATCAGTTTACCCAATTT pLKO_005 279 CDS 100% 13.200 9.240 N Ergic1 n/a
4 TRCN0000247355 CCATGAAGATCCCGCTGAATA pLKO_005 371 CDS 100% 13.200 9.240 N Ergic1 n/a
5 TRCN0000176165 GACATATACTGGTGCCATTAT pLKO.1 123 CDS 100% 13.200 9.240 N Ergic1 n/a
6 TRCN0000247356 GACCTCACACAGCCGACATAT pLKO_005 109 CDS 100% 13.200 9.240 N Ergic1 n/a
7 TRCN0000247354 GAACGGTGGAGACTCGGATTT pLKO_005 1541 3UTR 100% 10.800 7.560 N Ergic1 n/a
8 TRCN0000193691 CCATGACTACATCTTGAAGAT pLKO.1 612 CDS 100% 4.950 3.465 N Ergic1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006524823.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03808 pDONR223 100% 85.5% 93.1% None (many diffs) n/a
2 ccsbBroad304_03808 pLX_304 0% 85.5% 93.1% V5 (many diffs) n/a
3 TRCN0000469650 ATTGCAGCACATCCAGACATCGGC pLX_317 41.1% 85.5% 93.1% V5 (many diffs) n/a
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