Transcript: Mouse XM_006527965.3

PREDICTED: Mus musculus zinc finger protein X-linked (Zfx), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Zfx (22764)
Length:
6692
CDS:
29..2527

Additional Resources:

NCBI RefSeq record:
XM_006527965.3
NBCI Gene record:
Zfx (22764)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006527965.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000095620 ACAGAAATTGACCCTTGTAAA pLKO.1 818 CDS 100% 13.200 18.480 N Zfx n/a
2 TRCN0000095619 GCCCAGAGTTAATAAAGTAAT pLKO.1 6465 3UTR 100% 13.200 18.480 N Zfx n/a
3 TRCN0000095623 GACTGACTATGGACAACGAAA pLKO.1 798 CDS 100% 4.950 6.930 N Zfx n/a
4 TRCN0000095621 CGGTAATAATTCTGATGGAAT pLKO.1 1201 CDS 100% 4.950 2.970 N Zfx n/a
5 TRCN0000096494 CCTGATTCTATACTGAAGTTT pLKO.1 2766 3UTR 100% 5.625 2.813 Y Zfa-ps n/a
6 TRCN0000095622 GCCTATTGAATCGCCATCTTT pLKO.1 1704 CDS 100% 5.625 2.813 Y Zfx n/a
7 TRCN0000221679 AGTGATTTGAAACGACACATA pLKO.1 2045 CDS 100% 4.950 2.475 Y ZFX n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006527965.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01791 pDONR223 100% 78% 80% None (many diffs) n/a
2 TRCN0000477192 CGGTAAATAATATAGGTCCGTATT pLX_317 20.2% 78% 80% V5 (many diffs) n/a
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