Transcript: Mouse XM_006534137.2

PREDICTED: Mus musculus WNK lysine deficient protein kinase 4 (Wnk4), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Wnk4 (69847)
Length:
4282
CDS:
136..4026

Additional Resources:

NCBI RefSeq record:
XM_006534137.2
NBCI Gene record:
Wnk4 (69847)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006534137.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361782 ATGCTAGACTGGCACCTATAT pLKO_005 3068 CDS 100% 13.200 10.560 N Wnk4 n/a
2 TRCN0000028827 GATGCTAGACTGGCACCTATA pLKO.1 3067 CDS 100% 10.800 8.640 N Wnk4 n/a
3 TRCN0000028820 CCTCCCATTCTCTCCTAGTTA pLKO.1 2745 CDS 100% 5.625 4.500 N Wnk4 n/a
4 TRCN0000007021 CCGATACCTCAAGTTTGACAT pLKO.1 639 CDS 100% 4.950 3.960 N WNK4 n/a
5 TRCN0000028835 CGATACCTCAAGTTTGACATT pLKO.1 640 CDS 100% 4.950 3.960 N Wnk4 n/a
6 TRCN0000361841 CTCCCATTCTCTCCTAGTTAT pLKO_005 2746 CDS 100% 13.200 9.240 N Wnk4 n/a
7 TRCN0000361780 AGAATGAGGCTTCCCGTAATC pLKO_005 3026 CDS 100% 10.800 7.560 N Wnk4 n/a
8 TRCN0000028795 CCAGCTACTCATCAACTACAT pLKO.1 1847 CDS 100% 4.950 3.465 N Wnk4 n/a
9 TRCN0000028769 CCAGCATCAATCCTTCCTCTT pLKO.1 1818 CDS 100% 4.050 2.835 N Wnk4 n/a
10 TRCN0000199167 CCTGGCATCATGCGAAGGAAC pLKO.1 3697 CDS 100% 1.350 0.945 N WNK4 n/a
11 TRCN0000367529 ATTGCAGCTGCCATGGTATAT pLKO_005 2266 CDS 100% 13.200 9.240 N WNK4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006534137.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000489323 AGGCTACCCCTGATCACTCTATTA pLX_317 9.8% 77.1% 77.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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