Construct: ORF TRCN0000489323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020658.1_s317c1
- DNA Barcode:
- AGGCTACCCCTGATCACTCTATTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- WNK4 (65266)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489323
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65266 | WNK4 | WNK lysine deficient protei... | NM_032387.5 | 96.2% | 96.2% | 313C>A;2157_2294del |
| 2 | human | 65266 | WNK4 | WNK lysine deficient protei... | XM_017024962.1 | 91.9% | 91.8% | 313C>A;2157_2294del;3730_3906del |
| 3 | human | 65266 | WNK4 | WNK lysine deficient protei... | NM_001321299.2 | 69.2% | 63.2% | 0_1ins919;250_251ins89;1149_1286del |
| 4 | human | 65266 | WNK4 | WNK lysine deficient protei... | XM_017024966.1 | 66.1% | 60.3% | (many diffs) |
| 5 | mouse | 69847 | Wnk4 | WNK lysine deficient protei... | NM_175638.3 | 81.8% | 82.7% | (many diffs) |
| 6 | mouse | 69847 | Wnk4 | WNK lysine deficient protei... | XM_006534139.1 | 80.7% | 81.6% | (many diffs) |
| 7 | mouse | 69847 | Wnk4 | WNK lysine deficient protei... | XM_006534138.2 | 79.9% | 80.2% | (many diffs) |
| 8 | mouse | 69847 | Wnk4 | WNK lysine deficient protei... | XM_006534136.2 | 77.2% | 78% | (many diffs) |
| 9 | mouse | 69847 | Wnk4 | WNK lysine deficient protei... | XM_006534137.2 | 77.1% | 77.9% | (many diffs) |
| 10 | mouse | 69847 | Wnk4 | WNK lysine deficient protei... | XM_006534141.1 | 59.9% | 60.3% | (many diffs) |
| 11 | mouse | 69847 | Wnk4 | WNK lysine deficient protei... | XM_017314744.1 | 59.9% | 60.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 102
- ORF end:
- 3693
- ORF length:
- 3591
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcaccggat ccctcttcct catgttggca tccccggcca 121 cggagaccac cgtcctcatg tcccagactg aggccgacct ggccctgcgg cccccgcctc 181 ctcttggcac cgcggggcag ccccgcctcg ggccccctcc tcgccgagcg cgccgcttct 241 ccgggaaggc tgagccccgg ccgcgctctt ctcgtctcag ccgccgtagc tcagtcgact 301 tggggctgct gagctcttgg tccctgccag cctcacccgc tccggacccc cccgatcctc 361 cggactccgc tggtcctggc cccgcgagga gcccaccgcc tagctccaaa gaaacccccg 421 agggcacgtg gaccgaggga gcccctgtga aggctgcgga agactccgcg cgtcccgagc 481 tcccggactc tgcagtgggc ccggggtcca gggagccgct aagggtccct gaagctgtgg 541 ccctagagcg gcggcgggag caggaagaaa aggaggacat ggagacccag gctgtggcaa 601 cgtcccccga tggccgatac ctcaagtttg acatcgagat tggacgtggc tccttcaaga 661 cggtgtatcg agggctagac accgacacca cagtggaggt ggcctggtgt gagctgcaga 721 ctcggaaact gtctagagct gagcggcagc gcttctcaga ggaggtggag atgctcaagg 781 ggctgcagca ccccaacatc gtccgcttct atgattcgtg gaagtcggtg ctgaggggcc 841 aggtttgcat cgtgctggtc accgaactca tgacctcggg cacgctcaag acgtacctga 901 ggcggttccg ggagatgaag ccgcgggtcc ttcagcgctg gagccgccaa atcctgcggg 961 gacttcattt cctacactcc cgggttcctc ccatcctgca ccgggatctc aagtgcgaca 1021 atgtctttat cacgggacct actggctctg tcaaaatcgg ggacctgggc ctggccacgc 1081 tcaagcgcgc ctcctttgcc aagagtgtca tcgggacccc ggaattcatg gcccccgaga 1141 tgtacgagga aaagtacgat gaggccgtgg acgtgtacgc gttcggcatg tgcatgctgg 1201 agatggccac ctctgagtac ccgtactccg agtgccagaa tgccgcgcaa atctaccgca 1261 aggtcacttc gggcagaaag ccgaacagct tccacaaggt gaagataccc gaggtgaagg 1321 agatcattga aggctgcatc cgcacggata agaacgagag gttcaccatc caggacctcc 1381 tggcccacgc cttcttccgc gaggagcgcg gtgtgcacgt ggaactagcg gaggaggacg 1441 acggcgagaa gccgggcctc aagctctggc tgcgcatgga ggacgcgcgg cgcggggggc 1501 gcccacggga caaccaggcc atcgagttcc tgttccagct gggccgggac gcggccgagg 1561 aggtggcaca ggagatggtg gctctgggct tggtctgtga agccgattac cagccagtgg 1621 cccgtgcagt acgtgaacgg gttgctgcca tccagcgaaa gcgtgagaag ctgcgtaaag 1681 caagggaatt ggaggcactc ccaccagagc caggacctcc accagcaact gtgcccatgg 1741 cccccggtcc ccccagtgtc ttcccccctg agcctgagga gccagaggca gaccagcacc 1801 agcccttcct tttccgccac gccagctact catctaccac ttcggattgc gagactgatg 1861 gctacctcag ctcctccggc ttcctggatg cctcagaccc tgcccttcag ccccctgggg 1921 gggtgccatc cagcctggct gagtcccatc tctgcctgcc ctcggctttt gccctatcca 1981 ttccacgttc tggccctgga agtgactttt cccccgggga cagctatgcc tcagatgcag 2041 cttcaggcct tagcgatgtg ggagaaggga tgggacaaat gaggagaccc ccagggagga 2101 atctccggcg cagaccccga tcccggctgc gggtcactag tgtctcagac cagaatgaca 2161 gagtggttga gtgccagcta cagacccata acagcaagat ggtgaccttc cgatttgatc 2221 tggatgggga cagcccggaa gagattgcag ctgccatgga ggagccagca ccattacctg 2281 ccctgcccgt ccccctccca gacccatcca atgaagagct ccagagcagc acctccctgg 2341 agcacaggag ctggacagcc ttctccacct cctcatcttc tcctggaact cctttgtctc 2401 ctggaaaccc attttcccct ggaaccccca tttccccagg tcccatcttc cccatcactt 2461 ctcccccatg tcatcccagc ccctccccat tctcccccat ttcttcccag gtctcctcaa 2521 atccctctcc acaccccacc agctctccac ttccattctc ctccagcaca cccgagtttc 2581 cggtcccact ctctcagtgt ccctggagtt ctctccccac gacttctcca cctacgttct 2641 ctcccacttg ttctcaggtc actcttagtt cccctttctt tcctccgtgc ccctccactt 2701 cttccttccc ctccaccaca gcagcccctc tcctttctct ggctagtgcc ttctcactgg 2761 ctgtgatgac tgtggcccag tccctgctgt ccccctcacc tgggctcctt tcccagtctc 2821 ctccagcccc tcctagtccc ctccctagcc tgccccttcc ccctcccgtt gctcctggtg 2881 gccaggaaag cccttcaccc cacacagctg aggtggagag tgaggcctca ccacctcctg 2941 ctcggcccct cccaggggaa gccaggctgg cgcccatctc tgaagaggga aagccgcagc 3001 ttgttgggcg tttccaagtg acttcatcca aggaaccggc tgagcctctt cccttgcagc 3061 caacatcccc cactctctct ggttctccaa aaccttcaac ccctcagctc acttcagaga 3121 gctcagatac agaggacagt gctggaggcg ggccagagac cagggaagct ctggctgaga 3181 gcgaccgtgc agctgagggt ctgggggctg gagttgagga ggaaggagat gatgggaagg 3241 aaccccaagt tgggggcagc ccccaacccc tgagccatcc cagcccagtg tggatgaact 3301 actcctacag cagcctgtgt ttgagcagcg aggagtcaga aagcagtggg gaagatgagg 3361 agttctgGGC TGAGCTGCAG AGTCTTCGGC AGAAGCACTT GTCAGAGGTG GAAACACTAC 3421 AGACACTACA GAAAAAAGAA ATTGAAGATT TGTACAGCCG GCTGGGGAAG CAGCCCCCAC 3481 CGGGTATTGT GGCCCCAGCT GCTATGCTGT CCAGCCGCCA GCGCCGCCTC TCCAAGGGCA 3541 GCTTCCCCAC CTCCCGCCGC AACAGCCTAC AGCGCTCTGA GCCCCCAGGC CCTGGCATCA 3601 TGCGAAGGAA CTCTCTGAGT GGCAGCAGCA CCGGCTCCCA GGAGCAGCGG GCAAGCAAGG 3661 GGGTGACATT CGCCGGGGAT GTTGGCAGGA TGTGAAAGCT TAAGGGTGGG CGCGCCGACC 3721 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3841 ATATATCTTG TGGAAAGGAC GAAGGCTACC CCTGATCACT CTATTAACGC GTTAAGTCga 3901 caatcaacct ctggattaca aaatttgtga aagatt