Transcript: Mouse XM_006538702.3

PREDICTED: Mus musculus F-box protein 42 (Fbxo42), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fbxo42 (213499)
Length:
2556
CDS:
288..2324

Additional Resources:

NCBI RefSeq record:
XM_006538702.3
NBCI Gene record:
Fbxo42 (213499)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006538702.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000277616 CAAACAGTGGTATCGACTTAT pLKO_005 512 CDS 100% 13.200 18.480 N FBXO42 n/a
2 TRCN0000277424 CACACGTACTCGCCGTCTAAA pLKO_005 801 CDS 100% 13.200 18.480 N Fbxo42 n/a
3 TRCN0000277425 CGAGGCGAGCTCATCGTATTT pLKO_005 2223 CDS 100% 13.200 18.480 N Fbxo42 n/a
4 TRCN0000176730 CCATTCTCAATGGTGGAAATT pLKO.1 1480 CDS 100% 13.200 10.560 N Fbxo42 n/a
5 TRCN0000176980 GCTAGACATTAAAGACACCAA pLKO.1 2114 CDS 100% 2.640 2.112 N Fbxo42 n/a
6 TRCN0000277357 GCTAGACATTAAAGACACCAA pLKO_005 2114 CDS 100% 2.640 2.112 N Fbxo42 n/a
7 TRCN0000197775 GATGCCAATCAGTCTATGTAT pLKO.1 552 CDS 100% 5.625 3.938 N Fbxo42 n/a
8 TRCN0000277353 TTGCTCCAGGCTGGGATTAAA pLKO_005 2450 3UTR 100% 15.000 9.000 N Fbxo42 n/a
9 TRCN0000133892 CCAGAGAGATTCTTTGATGAA pLKO.1 777 CDS 100% 4.950 2.970 N FBXO42 n/a
10 TRCN0000197975 CCTAAATAGCAAGGAGTGGAT pLKO.1 635 CDS 100% 2.640 1.584 N Fbxo42 n/a
11 TRCN0000277426 CCTAAATAGCAAGGAGTGGAT pLKO_005 635 CDS 100% 2.640 1.584 N Fbxo42 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006538702.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03416 pDONR223 100% 83.8% 89.9% None (many diffs) n/a
2 ccsbBroad304_03416 pLX_304 0% 83.8% 89.9% V5 (many diffs) n/a
3 TRCN0000474599 AACAGTTTTAACCTAGCCTGCTGC pLX_317 19.1% 83.8% 89.9% V5 (many diffs) n/a
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