Construct: ORF TRCN0000474599
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000009.1_s317c1
- Derived from:
- ccsbBroadEn_03416
- DNA Barcode:
- AACAGTTTTAACCTAGCCTGCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXO42 (54455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474599
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54455 | FBXO42 | F-box protein 42 | NM_018994.3 | 99.9% | 100% | 15G>A |
| 2 | human | 54455 | FBXO42 | F-box protein 42 | XM_006710698.3 | 99.9% | 100% | 15G>A |
| 3 | human | 54455 | FBXO42 | F-box protein 42 | XM_017001502.1 | 67.7% | 67.7% | 0_1ins693 |
| 4 | human | 54455 | FBXO42 | F-box protein 42 | XM_017001503.1 | 67.7% | 67.7% | 0_1ins693 |
| 5 | human | 54455 | FBXO42 | F-box protein 42 | XR_001737235.1 | 35.5% | (many diffs) | |
| 6 | mouse | 213499 | Fbxo42 | F-box protein 42 | NM_172518.3 | 88.7% | 95.3% | (many diffs) |
| 7 | mouse | 213499 | Fbxo42 | F-box protein 42 | XM_006538702.3 | 83.8% | 89.9% | (many diffs) |
| 8 | mouse | 213499 | Fbxo42 | F-box protein 42 | XM_006538703.3 | 72% | 77.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2217
- ORF length:
- 2151
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagctcctca gacagtgaag atgacagttt catggctgtg gaccaggaag 121 aaactgtgct ggaagggaca atggatcaag atgaggagcc ccacccagta ttggaggctg 181 aggagactag acataatagg tccatgtcgg agctgccaga agaggttttg gagtatatcc 241 tgtcctttct ctcaccgtat caggaacaca aaactgcggc ccttgtctgc aaacagtggt 301 atcgacttat caaaggtgta gcccatcagt gttatcatgg tttcatgaag gctgtccagg 361 aaggaaacat tcagtgggag agccgtacct atccttatcc tggaacccca atcactcagc 421 gcttctcgca cagtgcatgc tattatgatg ctaatcagtc tatgtatgtg tttggaggct 481 gtacccagag cagctgcaat gctgctttca atgacctctg gagacttgac ctaaacagca 541 aagagtggat ccgacctttg gcttcagggt cctatccttc ccccaaagct ggagcaactc 601 tggtcgtgta caaggacttg ctagtgctgt ttggtggctg gacgcggcca agcccttatc 661 ccctacacca gccagagaga ttctttgatg aaatacacac ttactcaccc tctaaaaatt 721 ggtggaactg cattgtgaca acccatgggc cacctcccat ggctggccac tcctcctgtg 781 tgatagatga taaaatgatt gtctttggtg gctctttagg atcccggcaa atgagcaatg 841 atgtctgggt ccttgacctt gagcagtggg cgtggtccaa gccgaacatc tctggcccca 901 gtcctcatcc tcgaggtggc caatctcaga ttgtcataga tgatgcaact atcttaatcc 961 tcggagggtg tggcggtccc aatgctctat tcaaggatgc ttggttgttg cacatgcatt 1021 ctggtccttg ggcctggcag ccactcaagg tagaaaatga agagcatggg gccccagaac 1081 tgtggtgcca tccagcttgc cgggtgggac agtgtgtggt ggtcttcagc caggctccta 1141 gtgggagagc cccactcagc cccagtttga actctcgccc atcacctatc agtgccactc 1201 ctccagctct cgttcctgaa acccgagagt accgctctca gtctccagta agaagcatgg 1261 atgaagctcc ttgtgttaac ggccgctggg gaacactgag acccagggct caaaggcaga 1321 ctccttcagg ttcccgggaa gggagccttt ccccagccag aggagacggc tctcctatcc 1381 tcaatggtgg gagtttgtct ccaggaacgg cagctgtggg tggctcttct ttggacagtc 1441 ctgtacaggc catatctcca agtactccat ctgctcctga aggatacgac ctgaaaatag 1501 gactttcttt ggccccccga cgaggatcac taccagatca gaaagatctg agattaggat 1561 ccatagatct gaattgggat ctgaaacccg cttccagtag taatcccatg gatggcatgg 1621 acaataggac agttggggga agtatgagac accctcctga acagacaaat ggtgtgcata 1681 ccccacctca cgtggccagt gcccttgcag gggccgtctc cccaggtgcc ctgcgtcgga 1741 gtctggaagc catcaaagcg atgtcctcca aaggcccctc ggcctctgca gcactaagtc 1801 ctcctcttgg gTCTTCTCCA GGCTCTCCTG GGAGCCAGAG TTTGAGCAGT GGAGAAACAG 1861 TGCCCATCCC TCGCCCAGGG CCTGCCCAAG GAGATGGACA TTCCTTACCT CCCATTGCTC 1921 GCCGCCTGGG CCACCACCCT CCACAGTCCC TAAATGTTGG CAAACCCCTA TACCAGAGTA 1981 TGAACTGCAA GCCCATGCAG ATGTACGTGC TGGACATTAA AGACACCAAG GAGAAGGGGC 2041 GGGTCAAATG GAAAGTATTT AATAGCAGTT CTGTGGTTGG ACCTCCTGAA ACCAGCCTGC 2101 ATACCGTGGT ACAAGGCAGG GGTGAACTCA TCATATTTGG AGGACTCATG GACAAGAAAC 2161 AGAATGTGAA GTACTATCCA AAAACAAACG CCTTGTACTT TGTACGAGCA AAGAGATACC 2221 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2281 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2341 ATATATCTTG TGGAAAGGAC GAAACAGTTT TAACCTAGCC TGCTGCACGC GTTAAGTCga 2401 caatcaacct ctggattaca aaatttgtga aagatt