Transcript: Mouse XM_006540568.3

PREDICTED: Mus musculus ankyrin repeat and SOCS box-containing 7 (Asb7), transcript variant X3, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Asb7 (117589)
Length:
5070
CDS:
1070..1849

Additional Resources:

NCBI RefSeq record:
XM_006540568.3
NBCI Gene record:
Asb7 (117589)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006540568.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000241515 CACGGATGCTGTATAACTATG pLKO_005 1581 CDS 100% 10.800 15.120 N Asb7 n/a
2 TRCN0000190032 GACACAAACTTAGGGCGTCTA pLKO.1 1505 CDS 100% 4.050 5.670 N Asb7 n/a
3 TRCN0000241514 CAGGACCTGTGCCGAATTAAA pLKO_005 1727 CDS 100% 15.000 12.000 N Asb7 n/a
4 TRCN0000241516 ATCCCACGGTTAAGGATTTAA pLKO_005 1107 CDS 100% 15.000 10.500 N Asb7 n/a
5 TRCN0000241513 CCCACGCTTGAAGGTCTTAAT pLKO_005 2075 3UTR 100% 13.200 9.240 N Asb7 n/a
6 TRCN0000241517 GGTCATGAAAGACTACTTAAA pLKO_005 1807 CDS 100% 13.200 9.240 N Asb7 n/a
7 TRCN0000217732 GTGGACAAGATTACGAGTTAT pLKO.1 1862 3UTR 100% 13.200 9.240 N Asb7 n/a
8 TRCN0000200532 CCATAAGTATTTCAGGAAGTA pLKO.1 1653 CDS 100% 4.950 3.465 N Asb7 n/a
9 TRCN0000201637 CGCTCAGTGATAAAGGTACAA pLKO.1 1326 CDS 100% 4.950 3.465 N Asb7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006540568.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04935 pDONR223 100% 58% 63.1% None (many diffs) n/a
2 ccsbBroad304_04935 pLX_304 0% 58% 63.1% V5 (many diffs) n/a
3 TRCN0000469698 GCCTGTAATGGTGGACCAAATAAA pLX_317 50.7% 58% 63.1% V5 (many diffs) n/a
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