Construct: ORF TRCN0000469698
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018057.1_s317c1
- Derived from:
- ccsbBroadEn_04935
- DNA Barcode:
- GCCTGTAATGGTGGACCAAATAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASB7 (140460)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469698
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140460 | ASB7 | ankyrin repeat and SOCS box... | NM_024708.3 | 100% | 100% | |
| 2 | human | 140460 | ASB7 | ankyrin repeat and SOCS box... | NM_198243.3 | 85.8% | 85.5% | 819_821delACAinsTAT;823_954del |
| 3 | mouse | 117589 | Asb7 | ankyrin repeat and SOCS box... | XM_011250779.2 | 85% | 94.7% | (many diffs) |
| 4 | mouse | 117589 | Asb7 | ankyrin repeat and SOCS box... | NM_080443.2 | 76.4% | 85.2% | (many diffs) |
| 5 | mouse | 117589 | Asb7 | ankyrin repeat and SOCS box... | XM_006540567.3 | 76.4% | 85.2% | (many diffs) |
| 6 | mouse | 117589 | Asb7 | ankyrin repeat and SOCS box... | XM_006540568.3 | 58% | 63.1% | (many diffs) |
| 7 | mouse | 117589 | Asb7 | ankyrin repeat and SOCS box... | NR_003961.1 | 14.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 888
- ORF length:
- 822
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt acaccatcat tgtcgaagga accctgagct ccaggaagag ttgcagattc 121 aggccgcggt ggctgctggg gatgtccaca cagtgcgaaa gatgctagaa caaggctatt 181 ccccgaatgg ccgagatgcg aatggctgga ctctgcttca tttctctgca gcaagaggaa 241 aggaaagatg tgttcgggtt tttctagaac acggagctga tcctacagtt aaagacttaa 301 tcggaggctt cacggctctt cactatgcag ccatgcatgg ccgggcccgc attgcacgct 361 tgatgttaga atctgaatac aggagcgaca tcattaatgc aaaaagcaat gacggctgga 421 ctcccctcca tgtggctgcc cactacggca gggactcatt tgtccggctc cTCCTGGAGT 481 TCAAGGCTGA GGTTGACCCA CTCAGTGATA AAGGTACCAC ACCGCTTCAG CTCGCCATTA 541 TCCGAGAGAG GTCAAGCTGT GTGAAAATCC TCCTGGACCA CAATGCCAAC ATCGACATTC 601 AGAATGGTTT CCTGTTGCGA TACGCCGTGA TCAAAAGCAA TCACTCTTAT TGCCGAATGT 661 TCCTTCAGAG AGGGGCAGAC ACAAACTTGG GTCGCTTAGA AGACGGACAG ACTCCTTTAC 721 ACTTATCTGC CCTTAGGGAT GATGTGCTGT GTGCACGGAT GTTATATAAT TACGGAGCAG 781 ACACGAACAC ACGGAACTAT GAAGGACAGA CCCCATTGGC TGTTTCAATA AGTATTTCTG 841 GAAGTAGTCG ACCATGTTTG GATTTCTTAC AAGAAGTCAC AAGTATGTAC CCAACTTTCT 901 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 961 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1021 GTGGAAAGGA CGAGCCTGTA ATGGTGGACC AAATAAAACG CGTTAAGTCg acaatcaacc 1081 tctggattac aaaatttgtg aaagatt