Transcript: Mouse XM_006540665.3

PREDICTED: Mus musculus kinesin family member 7 (Kif7), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Kif7 (16576)
Length:
4961
CDS:
465..4511

Additional Resources:

NCBI RefSeq record:
XM_006540665.3
NBCI Gene record:
Kif7 (16576)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006540665.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000090439 CGAGTACAAGAATGAGGCTAT pLKO.1 3647 CDS 100% 4.050 3.240 N Kif7 n/a
2 TRCN0000090441 CGTGCTCAGAACATCCGCAAT pLKO.1 1497 CDS 100% 4.050 3.240 N Kif7 n/a
3 TRCN0000090438 GCCCAGAGTTCTGGAGATAAT pLKO.1 4801 3UTR 100% 13.200 9.240 N Kif7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006540665.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13627 pDONR223 100% 52.1% 52.9% None (many diffs) n/a
2 ccsbBroad304_13627 pLX_304 0% 52.1% 52.9% V5 (many diffs) n/a
3 TRCN0000476193 GACCTCCTATACTGGGTTCTGTCC pLX_317 12.5% 52.1% 52.9% V5 (many diffs) n/a
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