Construct: ORF TRCN0000476193

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008538.1_s317c1
Derived from:
ccsbBroadEn_13627
DNA Barcode:
GACCTCCTATACTGGGTTCTGTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIF7 (374654)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476193
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 374654 KIF7 kinesin family member 7 NM_198525.3 61.7% 61.6% (many diffs)
2 human 374654 KIF7 kinesin family member 7 XM_011521531.2 59.8% 59.8% (many diffs)
3 mouse 16576 Kif7 kinesin family member 7 XM_006540670.3 79.1% 80.2% (many diffs)
4 mouse 16576 Kif7 kinesin family member 7 XM_006540667.1 52.1% 52.8% (many diffs)
5 mouse 16576 Kif7 kinesin family member 7 NM_001291222.1 52.1% 52.9% (many diffs)
6 mouse 16576 Kif7 kinesin family member 7 XM_006540664.1 52.1% 52.9% (many diffs)
7 mouse 16576 Kif7 kinesin family member 7 XM_006540665.3 52.1% 52.9% (many diffs)
8 mouse 16576 Kif7 kinesin family member 7 XM_006540666.1 52.1% 52.8% (many diffs)
9 mouse 16576 Kif7 kinesin family member 7 NM_010626.3 52% 52.8% (many diffs)
10 mouse 16576 Kif7 kinesin family member 7 XM_006540668.1 52% 52.8% (many diffs)
11 mouse 16576 Kif7 kinesin family member 7 XM_006540669.3 28.9% 28.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2559
ORF length:
2490
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagcagtac aaactgcaga gcgaccggct gcgtgagcag caggaggaga
121tggtggaact gcggctgcgg ttagagctgg tgcggccagg ctgggggggc ccgcggctcc
181tgaatggcct gcctcccggg tcctttgtgc ctcgacctca tacagccccc ctggggggtg
241cccacgccca tgtgctgggc atggtgccgc ctgcctgcct ccctggagat gaagttggct
301ctgagcagag gggagagcag gtgacaaatg gcagggaggc tggagctgag ttgctgactg
361aggtgaacag gctgggaagt ggctcttcag ctgcttcaga ggaggaagag gaggaggagg
421agccgcccag gcggacctta cacctgcgca gaaataggat cagcaactgc agtcagaggg
481cgggggcacg cccagggagt ctgccagaga ggaagggccc agagctttgc cttgaggagt
541tggatgcagc cattccaggg tccagagcag ttggtgggag caaggcccga gttcaggccc
601gccaggtccc ccctgccaca gcctcagagt ggcggctggc ccaggcccag cagaagatcc
661gggagctggc tatcaacatc cgcatgaagg aggagcttat tggcgagctg gtccgcacag
721gaaaggcagc tcaggccctg aaccgccagc acagccagcg tatccgggag ctggagcagg
781aggcagagca ggtgcgggcc gagctgagtg aaggccagag gcagctgcgg gagctcgagg
841gcaaggagct ccaggatgct ggcgagcggt ctcggctcca ggagttccgc aggagggtcg
901ctgcggccca gagccaggtg caggtgctga aggagaagaa gcaggctacg gagcggctgg
961tgtcactgtc ggcccagagt gagaagcgac tgcaggagct cgagcggaac gtgcagctca
1021tgcggcagca gcagggacag ctgcagaggc ggcttcgcga ggagacggag cagaagcggc
1081gcctggaggc agaaatgagc aagcggcagc accgcgtcaa ggagctggag ctgaagcatg
1141agcaacagca gaagatcctg aagattaaga cggaagagat cgcggccttc cagaggaaga
1201ggcgcagtgg cagcaacggc tctgtggtca gcctggaaca gcagcagaag attgaggagc
1261agaagaagtg gctggaccag gagatggaga aggtgctaca gcagcggcgg gcgctggagg
1321agctggggga ggagctccac aagcgggagg ccatcctggc caagaaggag gccctgatgc
1381aggagaagac ggggctggag atcaagcgcc tgagatccag ccaggccctc aacgaggaca
1441tcgtgcgagt gtccagccgg ctggagcacc tggagaagga gctgtccgag aagagcgggc
1501agctgcggca gggcagcgcc cagagccagc agcagatccg cagggagatc gacagcctgc
1561gccaggagaa ggactcactg ctcaagcagc gcctggagat cgacggcaag ctgaggcagg
1621ggagtctgct gtcccccgag gaggagcgga cgctgttcca gttggatgag gccatcgagg
1681ccctggatgc tgccattgag tataagaatg aggccatcac atgccgccag cgggtgcttc
1741gggcctcagc ctcgttgctg tcccagtgcg agatgaacct catggccaag ctcagctacc
1801tctcatcctc agagaccaga gccctcctct gcaagtattt tgacaaggtg gtgacgctcc
1861gagaggagca gcaccagcag cagattgcct tctcggaact ggagatgcag ctggaggagc
1921agcagaggct ggtgtactgg ctggaggtgg ccctggagcg gcagcgcctg gagatggacc
1981gccagctgac cctgcagcag aaggagcacg agcagaacat gcagctgctc ctgcagcaga
2041gtcgagacca cctcggtgaa gggttagcag acagcaggag gcagtatgag gcccggattc
2101aagctctgga gaaggaactg ggccgttaca tgtggataaa ccaggaactg aaacagaagc
2161tcggcggtgt gaacgctgta ggccacagca ggggtgggga gaagaggagc ctgtgctcgg
2221agggcagaca ggctcctgga aatgaagatg agcTCCACCT GGCACCCGAG CTTCTCTGGC
2281TGTCCCCCCT CACTGAGGGG GCCCCCCGCA CCCGGGAGGA GACGCGGGAC TTGGTCCACG
2341CTCCGTTACC CTTGACCTGG AAACGCTCGA GCCTGTGTGG TGAGGAGCAG GGGTCCCCCG
2401AGGAACTGAG GCAGCGGGAG GCGGCTGAGC CCCTGGTGGG GCGGGTGCTT CCTGTGGGTG
2461AGGCAGGCCT GCCCTGGAAC TTTGGGCCTT TGTCCAAGCC CCGGCGGGAA CTGCGACGAG
2521CCAGCCCGGG GATGATTGAT GTCCGGAAAA ACCCCCTGTT GCCAACTTTC TTGTACAAAG
2581TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2641GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2701ACGAGACCTC CTATACTGGG TTCTGTCCAC GCGTTAAGTC gacaatcaac ctctggatta
2761caaaatttgt gaaagatt

Download FASTA (ORF) (Full)