Construct: ORF TRCN0000476193
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008538.1_s317c1
- Derived from:
- ccsbBroadEn_13627
- DNA Barcode:
- GACCTCCTATACTGGGTTCTGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF7 (374654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476193
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 374654 | KIF7 | kinesin family member 7 | NM_198525.3 | 61.7% | 61.6% | (many diffs) |
| 2 | human | 374654 | KIF7 | kinesin family member 7 | XM_011521531.2 | 59.8% | 59.8% | (many diffs) |
| 3 | mouse | 16576 | Kif7 | kinesin family member 7 | XM_006540670.3 | 79.1% | 80.2% | (many diffs) |
| 4 | mouse | 16576 | Kif7 | kinesin family member 7 | XM_006540667.1 | 52.1% | 52.8% | (many diffs) |
| 5 | mouse | 16576 | Kif7 | kinesin family member 7 | NM_001291222.1 | 52.1% | 52.9% | (many diffs) |
| 6 | mouse | 16576 | Kif7 | kinesin family member 7 | XM_006540664.1 | 52.1% | 52.9% | (many diffs) |
| 7 | mouse | 16576 | Kif7 | kinesin family member 7 | XM_006540665.3 | 52.1% | 52.9% | (many diffs) |
| 8 | mouse | 16576 | Kif7 | kinesin family member 7 | XM_006540666.1 | 52.1% | 52.8% | (many diffs) |
| 9 | mouse | 16576 | Kif7 | kinesin family member 7 | NM_010626.3 | 52% | 52.8% | (many diffs) |
| 10 | mouse | 16576 | Kif7 | kinesin family member 7 | XM_006540668.1 | 52% | 52.8% | (many diffs) |
| 11 | mouse | 16576 | Kif7 | kinesin family member 7 | XM_006540669.3 | 28.9% | 28.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2559
- ORF length:
- 2490
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcagtac aaactgcaga gcgaccggct gcgtgagcag caggaggaga 121 tggtggaact gcggctgcgg ttagagctgg tgcggccagg ctgggggggc ccgcggctcc 181 tgaatggcct gcctcccggg tcctttgtgc ctcgacctca tacagccccc ctggggggtg 241 cccacgccca tgtgctgggc atggtgccgc ctgcctgcct ccctggagat gaagttggct 301 ctgagcagag gggagagcag gtgacaaatg gcagggaggc tggagctgag ttgctgactg 361 aggtgaacag gctgggaagt ggctcttcag ctgcttcaga ggaggaagag gaggaggagg 421 agccgcccag gcggacctta cacctgcgca gaaataggat cagcaactgc agtcagaggg 481 cgggggcacg cccagggagt ctgccagaga ggaagggccc agagctttgc cttgaggagt 541 tggatgcagc cattccaggg tccagagcag ttggtgggag caaggcccga gttcaggccc 601 gccaggtccc ccctgccaca gcctcagagt ggcggctggc ccaggcccag cagaagatcc 661 gggagctggc tatcaacatc cgcatgaagg aggagcttat tggcgagctg gtccgcacag 721 gaaaggcagc tcaggccctg aaccgccagc acagccagcg tatccgggag ctggagcagg 781 aggcagagca ggtgcgggcc gagctgagtg aaggccagag gcagctgcgg gagctcgagg 841 gcaaggagct ccaggatgct ggcgagcggt ctcggctcca ggagttccgc aggagggtcg 901 ctgcggccca gagccaggtg caggtgctga aggagaagaa gcaggctacg gagcggctgg 961 tgtcactgtc ggcccagagt gagaagcgac tgcaggagct cgagcggaac gtgcagctca 1021 tgcggcagca gcagggacag ctgcagaggc ggcttcgcga ggagacggag cagaagcggc 1081 gcctggaggc agaaatgagc aagcggcagc accgcgtcaa ggagctggag ctgaagcatg 1141 agcaacagca gaagatcctg aagattaaga cggaagagat cgcggccttc cagaggaaga 1201 ggcgcagtgg cagcaacggc tctgtggtca gcctggaaca gcagcagaag attgaggagc 1261 agaagaagtg gctggaccag gagatggaga aggtgctaca gcagcggcgg gcgctggagg 1321 agctggggga ggagctccac aagcgggagg ccatcctggc caagaaggag gccctgatgc 1381 aggagaagac ggggctggag atcaagcgcc tgagatccag ccaggccctc aacgaggaca 1441 tcgtgcgagt gtccagccgg ctggagcacc tggagaagga gctgtccgag aagagcgggc 1501 agctgcggca gggcagcgcc cagagccagc agcagatccg cagggagatc gacagcctgc 1561 gccaggagaa ggactcactg ctcaagcagc gcctggagat cgacggcaag ctgaggcagg 1621 ggagtctgct gtcccccgag gaggagcgga cgctgttcca gttggatgag gccatcgagg 1681 ccctggatgc tgccattgag tataagaatg aggccatcac atgccgccag cgggtgcttc 1741 gggcctcagc ctcgttgctg tcccagtgcg agatgaacct catggccaag ctcagctacc 1801 tctcatcctc agagaccaga gccctcctct gcaagtattt tgacaaggtg gtgacgctcc 1861 gagaggagca gcaccagcag cagattgcct tctcggaact ggagatgcag ctggaggagc 1921 agcagaggct ggtgtactgg ctggaggtgg ccctggagcg gcagcgcctg gagatggacc 1981 gccagctgac cctgcagcag aaggagcacg agcagaacat gcagctgctc ctgcagcaga 2041 gtcgagacca cctcggtgaa gggttagcag acagcaggag gcagtatgag gcccggattc 2101 aagctctgga gaaggaactg ggccgttaca tgtggataaa ccaggaactg aaacagaagc 2161 tcggcggtgt gaacgctgta ggccacagca ggggtgggga gaagaggagc ctgtgctcgg 2221 agggcagaca ggctcctgga aatgaagatg agcTCCACCT GGCACCCGAG CTTCTCTGGC 2281 TGTCCCCCCT CACTGAGGGG GCCCCCCGCA CCCGGGAGGA GACGCGGGAC TTGGTCCACG 2341 CTCCGTTACC CTTGACCTGG AAACGCTCGA GCCTGTGTGG TGAGGAGCAG GGGTCCCCCG 2401 AGGAACTGAG GCAGCGGGAG GCGGCTGAGC CCCTGGTGGG GCGGGTGCTT CCTGTGGGTG 2461 AGGCAGGCCT GCCCTGGAAC TTTGGGCCTT TGTCCAAGCC CCGGCGGGAA CTGCGACGAG 2521 CCAGCCCGGG GATGATTGAT GTCCGGAAAA ACCCCCTGTT GCCAACTTTC TTGTACAAAG 2581 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2641 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2701 ACGAGACCTC CTATACTGGG TTCTGTCCAC GCGTTAAGTC gacaatcaac ctctggatta 2761 caaaatttgt gaaagatt