Transcript: Human XM_006710345.3

PREDICTED: Homo sapiens ankyrin repeat and SOCS box containing 17 (ASB17), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ASB17 (127247)
Length:
939
CDS:
117..860

Additional Resources:

NCBI RefSeq record:
XM_006710345.3
NBCI Gene record:
ASB17 (127247)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006710345.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000433788 ATGCTCCCAGATGGAATATTT pLKO_005 789 CDS 100% 15.000 21.000 N ASB17 n/a
2 TRCN0000135384 GCTCAGACAAGACAGTCTAAT pLKO.1 435 CDS 100% 13.200 9.240 N ASB17 n/a
3 TRCN0000422676 GTAATGGTTGATCGTGAATTG pLKO_005 552 CDS 100% 10.800 7.560 N ASB17 n/a
4 TRCN0000134255 CTTTGTGGAATTGCTTCTCAA pLKO.1 299 CDS 100% 4.950 3.465 N ASB17 n/a
5 TRCN0000135622 GTACTATGTTCCAGAGTGCTT pLKO.1 606 CDS 100% 2.640 1.848 N ASB17 n/a
6 TRCN0000133896 CTTCTTGTCCAAGAAGCAATA pLKO.1 148 CDS 100% 1.080 0.756 N ASB17 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006710345.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04824 pDONR223 100% 83.7% 83.7% None 165_166ins144 n/a
2 ccsbBroad304_04824 pLX_304 0% 83.7% 83.7% V5 165_166ins144 n/a
3 TRCN0000468986 ATGAATTATAACCACCTATTCCTG pLX_317 50.1% 83.7% 83.7% V5 165_166ins144 n/a
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