Transcript: Human XM_006710397.3

PREDICTED: Homo sapiens dihydropyrimidine dehydrogenase (DPYD), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DPYD (1806)
Length:
5502
CDS:
138..2807

Additional Resources:

NCBI RefSeq record:
XM_006710397.3
NBCI Gene record:
DPYD (1806)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006710397.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000427194 ACTGAAGAGGGACCCATTAAT pLKO_005 570 CDS 100% 15.000 21.000 N DPYD n/a
2 TRCN0000413233 GGCCGGATTGAAGTTTATAAA pLKO_005 1748 CDS 100% 15.000 21.000 N DPYD n/a
3 TRCN0000221386 GCAATTTGCTACTGAGGTATT pLKO.1 605 CDS 100% 10.800 15.120 N DPYD n/a
4 TRCN0000221382 GCTGTCCAACTAATCTTGATA pLKO.1 406 CDS 100% 5.625 7.875 N DPYD n/a
5 TRCN0000433515 GTGTAGGTGGATGCAATTTAT pLKO_005 544 CDS 100% 15.000 10.500 N DPYD n/a
6 TRCN0000221385 GCCGTATGATGTAGTGAATTT pLKO.1 845 CDS 100% 13.200 9.240 N DPYD n/a
7 TRCN0000428798 ACATTGTGATTGCTAGCATTA pLKO_005 2041 CDS 100% 10.800 7.560 N DPYD n/a
8 TRCN0000424128 AGCAAGCTTCTTGGTACATTC pLKO_005 1627 CDS 100% 10.800 7.560 N DPYD n/a
9 TRCN0000221383 CGGCTGCATATTGGTGTCAAA pLKO.1 1987 CDS 100% 4.950 3.465 N DPYD n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006710397.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00459 pDONR223 100% 19% 18.2% None (many diffs) n/a
2 ccsbBroad304_00459 pLX_304 0% 19% 18.2% V5 (many diffs) n/a
3 TRCN0000475366 GACCTTCGACGGTTGATCCGTAAA pLX_317 19.8% 19% 18.2% V5 (many diffs) n/a
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