Construct: ORF TRCN0000475366
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014671.1_s317c1
- Derived from:
- ccsbBroadEn_00459
- DNA Barcode:
- GACCTTCGACGGTTGATCCGTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPYD (1806)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475366
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1806 | DPYD | dihydropyrimidine dehydroge... | NM_001160301.1 | 100% | 100% | |
| 2 | human | 1806 | DPYD | dihydropyrimidine dehydroge... | XR_001737014.1 | 66.7% | 1_137del;657_778del | |
| 3 | human | 1806 | DPYD | dihydropyrimidine dehydroge... | XM_006710397.3 | 19% | 18.2% | (many diffs) |
| 4 | human | 1806 | DPYD | dihydropyrimidine dehydroge... | XM_005270562.3 | 17.7% | 17% | (many diffs) |
| 5 | human | 1806 | DPYD | dihydropyrimidine dehydroge... | NM_000110.4 | 16.5% | 15.8% | (many diffs) |
| 6 | human | 1806 | DPYD | dihydropyrimidine dehydroge... | XM_017000507.1 | 12.9% | 12.2% | (many diffs) |
| 7 | mouse | 99586 | Dpyd | dihydropyrimidine dehydroge... | XM_011240318.2 | 25.2% | 25.2% | (many diffs) |
| 8 | mouse | 99586 | Dpyd | dihydropyrimidine dehydroge... | NM_170778.2 | 14.3% | 14.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 585
- ORF length:
- 519
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccctgtgctc agtaaggact cggcggacat cgagagtatc ctggctttaa 121 atcctcgaac acaaactcat gcaactctgt gttccacttc ggccaagaaa ttagacaaga 181 aacattggaa aagaaatcct gataagaact gctttaattg tgagaagctg gagaataatt 241 ttgatgacat caagcacacg actcttggtg agcgaggagc tctccgagaa gcaatgagat 301 gcctgaaatg tgcagatgcc ccgtgtcaga agagctgtcc aactaatctt gatattaaat 361 cattcatcac aagtattgca aacaagaact attatggagc tgctaagatg atattttctg 421 acaacccact tggtctgact tgtggaatgg tatgtccaac ctctgatctt tgtgtaggtg 481 gatgcaatTT ATATGCCACT GAAGAGGGAC CCATTAATAT TGGTGGATTG CAGCAATTTG 541 CTACTGAGAC TTTGATCCTG GCTTTCTCTT TAATGAATCA TTTGTACCCA ACTTTCTTGT 601 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 661 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 721 GAAAGGACGA GACCTTCGAC GGTTGATCCG TAAAACGCGT TAAGTCgaca atcaacctct 781 ggattacaaa atttgtgaaa gatt