Transcript: Human XM_006712955.3

PREDICTED: Homo sapiens nischarin (NISCH), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NISCH (11188)
Length:
5493
CDS:
1911..4907

Additional Resources:

NCBI RefSeq record:
XM_006712955.3
NBCI Gene record:
NISCH (11188)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006712955.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256839 ACTCTAGTCGCGTCAAGTTTA pLKO_005 4324 CDS 100% 13.200 18.480 N NISCH n/a
2 TRCN0000162814 CGACTACAACAACAGCCCTTT pLKO.1 4028 CDS 100% 4.050 5.670 N NISCH n/a
3 TRCN0000256840 ACAGGCAGCTTCCGATGATTT pLKO_005 2042 CDS 100% 13.200 10.560 N NISCH n/a
4 TRCN0000256843 CTGTTGTGGGACCGTTGTTAA pLKO_005 5164 3UTR 100% 13.200 9.240 N NISCH n/a
5 TRCN0000163146 GCTGAGAACCGCTACTTTGAA pLKO.1 2340 CDS 100% 5.625 3.938 N NISCH n/a
6 TRCN0000162969 GCCTGTTGACAAGGACTTCTA pLKO.1 4250 CDS 100% 4.950 3.465 N NISCH n/a
7 TRCN0000163059 CCAGGGATGTTCTGATTCCTT pLKO.1 2003 CDS 100% 3.000 2.100 N NISCH n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006712955.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07768 pDONR223 100% 66.1% 66% None (many diffs) n/a
2 ccsbBroad304_07768 pLX_304 0% 66.1% 66% V5 (many diffs) n/a
3 TRCN0000476629 CCAAAACAGGGGTCACAGGAATCA pLX_317 8.3% 66.1% 66% V5 (many diffs) n/a
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