Construct: ORF TRCN0000476629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012706.1_s317c1
- Derived from:
- ccsbBroadEn_07768
- DNA Barcode:
- CCAAAACAGGGGTCACAGGAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NISCH (11188)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11188 | NISCH | nischarin | NM_007184.4 | 99.9% | 99.8% | (many diffs) |
| 2 | human | 11188 | NISCH | nischarin | XR_001739989.1 | 82.1% | (many diffs) | |
| 3 | human | 11188 | NISCH | nischarin | XM_005264839.3 | 73.9% | 73.8% | 0_1ins1173;1608G>C;1994C>T |
| 4 | human | 11188 | NISCH | nischarin | XM_017005643.2 | 73.9% | 73.8% | 0_1ins1173;1608G>C;1994C>T |
| 5 | human | 11188 | NISCH | nischarin | XM_006712955.3 | 66.1% | 66% | (many diffs) |
| 6 | human | 11188 | NISCH | nischarin | NM_001276293.2 | 36.8% | 34.4% | (many diffs) |
| 7 | human | 11188 | NISCH | nischarin | NM_001276294.2 | 34% | 33.9% | (many diffs) |
| 8 | mouse | 64652 | Nisch | nischarin | NM_022656.2 | 80.6% | 84.2% | (many diffs) |
| 9 | mouse | 64652 | Nisch | nischarin | XM_006519353.2 | 53.6% | 55.5% | (many diffs) |
| 10 | mouse | 64652 | Nisch | nischarin | XM_017316115.1 | 53.6% | 55.5% | (many diffs) |
| 11 | mouse | 64652 | Nisch | nischarin | XM_017316116.1 | 53.6% | 55.5% | (many diffs) |
| 12 | mouse | 64652 | Nisch | nischarin | NM_001347583.1 | 29% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4578
- ORF length:
- 4512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaccgcgcgc accttcgggc ccgagcggga agccgagccg gccaaggaag 121 cgcgcgtcgt gggctcggag cttgtggaca cttatacggt ttacatcatc caggtcactg 181 atggcagcca tgagtggaca gtaaagcacc gctacagcga cttccatgac ctgcatgaaa 241 agctcgttgc agagagaaag attgataaaa acctgcttcc gcccaaaaag ataattggga 301 aaaactcaag aagcttggtg gagaagaggg agaaggatct ggaggtctac ctccagaagc 361 tcctggctgc cttccctggc gtgaccccca gagtactggc ccacttcttg cattttcact 421 tctatgagat aaatggcatc accgcggcac tggctgaaga gctctttgag aaaggagaac 481 agctcctggg ggccggcgag gtctttgcca ttggacccct gcagctgtat gccgtcacgg 541 agcagctgca gcagggaaag cccacgtgcg ccagtgggga tgccaagacc gacctcgggc 601 acatcctgga cttcacctgt cgccttaagt accttaaggt ttctggcaca gaaggacctt 661 ttgggaccag caacattcag gagcagctcc tgccgttcga cctatcaata ttcaagtccc 721 tgcatcaggt ggagataagt cactgtgatg ctaagcacat cagagggctg gtcgcatcga 781 agcccacctt agccacgctg agtgtccgct tctcagcaac ctcgatgaag gaagtccttg 841 ttcctgaagc ctcagaattt gatgagtggg agcctgaagg cacaacccta gaaggccctg 901 tgactgccgt catccccact tggcaggcat tgaccacgct tgacctgagc cacaacagca 961 tctccgagat cgacgagtct gtgaaactga tcccaaagat tgagttcctg gacctgagtc 1021 acaatggatt gctggttgtg gacaatctgc agcacctgta taaccttgtg catctggacc 1081 tgtcctacaa caagctctcc tccttggaag ggcttcacac caagctgggg aacatcaaga 1141 ccttaaacct ggcaggcaac ctcctagaga gtctgagtgg cctgcacaag ctctactcac 1201 tggtcaacct ggatctccgg gacaacagga tcgaacagat ggaggaggtc cggagcatag 1261 gcagcctccc gtgtctggag cacgtgtctc tgctgaacaa ccctctgagc atcatccccg 1321 actaccggac caaggtgctg gctcagttcg gagagagggc ctcagaggtc tgtctggatg 1381 acacagtgac cacagagaag gagctggaca ctgtggaagt gctgaaagca attcagaaag 1441 ccaaggaggt caagtccaaa ctgagcaacc cagagaagaa gggtggtgaa gactcccggc 1501 tctcagctgc cccctgcatc agacccagca gctcccctcc cactgtggct cccgcatctg 1561 cctccctgcc ccagcccatc ctctctaacc aaggaatcat gttcgttcag gaggaggccc 1621 tggccagcag cctctcgtcc actgacagtc tgactcccga gcaccagccc attgcccagg 1681 gatgttctga ttccttggag tccatccctg cgggacaggc agcttccgat gatttaaggg 1741 acgtgccagg agctgttggt ggtgcaagcc cagaacatgc cgagccggag gtccaggtgg 1801 tgccggggtc tggccagatc atcttcctgc ccttcacctg cattggctac acggccacca 1861 atcaggactt catccagcgc ctgagcacac tgatccggca ggccatcgag cggcagctgc 1921 ctgcctggat cgaggctgcc aaccagcggg aggagggcca gggtgaacag ggcgaggagg 1981 aggatgagga ggaggaagaa gaggaggacg tggctgagaa ccgctacttt gaaatggggc 2041 ccccagacgt ggaggaggag gagggaggag gccaggggga ggaagaggag gaggaagagg 2101 aggatgaaga ggccgaggag gagcgcctgg ctctggaatg ggccctgggc gcggacgagg 2161 acttcctgct ggagcacatc cgcatcctca aggtgctgtg gtgcttcctg atccatgtgc 2221 agggcagtat ccgccagttc gccgcctgcc ttgtgctcac cgacttcggc atcgcagtct 2281 tcgagatccc gcaccaggag tctcggggca gcagccagca catcctctcc tccctgcgct 2341 ttgtcttttg cttcccgcat ggcgacctca ccgagtttgg cttcctcatg ccggagctgt 2401 gtctggtgct caaggtacgg cacagtgaga acacgctctt cattatctcg gacgccgcca 2461 acctgcacga gttccacgcg gacctgcgct catgctttgc accccagcac atggccatgc 2521 tgtgtagccc catcctctac ggcagccaca ccagcctgca ggagttcctg cgccagctgc 2581 tcaccttcta caaggtggct ggcggctgcc aggagcgcag ccagggctgc ttccccgtct 2641 acctggtcta cagtgacaag cgcatggtgc agacggccgc cggggactac tcaggcaaca 2701 tcgagtgggc cagctgcaca ctctgttcag ccgtgcggcg ctcctgctgc gcgccctctg 2761 aggccgtcaa gtccgccgcc atcccctact ggctgttgct cacgccccag cacctcaacg 2821 tcatcaaggc cgacttcaac cccatcccca accgtggcac ccacaactgt cgcaaccgca 2881 acagcttcaa gctcagccgt gtgccgctct ccaccgtgct gctggacccc acacgcagct 2941 gtacccagcc tcggggcgcc tttgctgatg gccacgtgct agagctgctc gtggggtacc 3001 gctttgtcac tgccatcttc gtgctgcccc acgagaagtt ccacttcctg cgcgtctaca 3061 accagctgcg ggcctcgctg caggacctga agactgtggt catcgccaag acccccggga 3121 cgggaggcag cccccagggc tcctttgcgg atggccagcc tgccgagcgc agggccagca 3181 atgaccagcg tccccaggag gtcccagcag aggctctggc cccggcccca gtggaagtcc 3241 cagctccagc ccctgcagca gcctcagcct caggcccagc gaagactccg gccccagcag 3301 aggcctcaac ttcagctttg gtcccagagg agacgccagt ggaagctcca gccccacccc 3361 cagccgaggc ccctgcccag tacccgagtg agcacctcat ccaggccacc tcggaggaga 3421 atcagatccc ctcgcacttg cctgcctgcc cgtcgctccg gcacgtcgcc agcctgcggg 3481 gcagcgccat catcgagctc ttccacagca gcattgctga ggttgaaaac gaggagctga 3541 ggcacctcat gtggtcctcg gtggtgttct accagacccc agggctggag gtgactgcct 3601 gcgtgctgct ctccaccaag gctgtgtact ttgtgctcca cgacggcctc cgccgctact 3661 tctcagagcc actgcaggat ttctggcatc agaaaaacac cgactacaac aacagccctt 3721 tccacatctc ccagtgcttc gtgctaaagc ttagtgacct gcagtcagtc aatgtggggc 3781 ttttcgacca gcatttccgg ctgacgggtt ccaccccgat gcaggtggtc acgtgcttga 3841 cgcgggacag ctacctgacg cactgcttcc tccagcacct catggtcgtg ctgtcctctc 3901 tggaacgcac gccctcgccg gagcctgttg acaaggactt ctactccgag tttgggaaca 3961 agaccacagg gaagatggag aactacgagc tgatccactc tagtcgcgtc aagtttacct 4021 accccagtga ggaggagatt ggggacctga cgttcactgt ggcccaaaag atggctgagc 4081 cagagaaggc cccagccctc agcatcctgc tgtacgtgca ggccttccag gtgggcatgc 4141 caccccctgg gtgctgcagg ggccccctgc gccccaagac actcctgctc accagctccg 4201 agatcttccT CCTGGATGAG GACTGTGTCC ACTACCCACT GCCCGAGTTT GCCAAAGAGC 4261 CGCCGCAGAG AGACAGGTAC CGGCTGGACG ATGGCCGCCG CGTCCGGGAC CTGGACCGAG 4321 TGCTCATGGG CTACCAGACC TACCCGCAGG CCCTCACCCT CGTCTTCGAT GACGTGCAAG 4381 GTCATGACCT CATGGGCAGT GTCACCCTGG ACCACTTTGG GGAGGTGCCA GGTGGCCCGG 4441 CTAGAGCCAG CCAGGGCCGT GAAGTCCAGT GGCAGGTGTT TGTCCCCAGT GCTGAGAGCA 4501 GAGAGAAGCT CATCTCGCTG TTGGCTCGCC AGTGGGAGGC CCTGTGTGGC CGTGAGCTGC 4561 CTGTCGAGCT CACCGGCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 4621 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 4681 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCAAAAC AGGGGTCACA 4741 GGAATCAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt