Transcript: Mouse XM_011246998.1

PREDICTED: Mus musculus AFG3-like AAA ATPase 2 (Afg3l2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Afg3l2 (69597)
Length:
4127
CDS:
1435..3609

Additional Resources:

NCBI RefSeq record:
XM_011246998.1
NBCI Gene record:
Afg3l2 (69597)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011246998.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000241172 GGCAATACGTCTGGTTTAATA pLKO_005 1823 CDS 100% 15.000 21.000 N Afg3l2 n/a
2 TRCN0000241173 TGTTGCCCACCGTACTCATTA pLKO_005 1967 CDS 100% 13.200 18.480 N Afg3l2 n/a
3 TRCN0000241174 CTTTGTCAATAACTATCTTTC pLKO_005 1719 CDS 100% 10.800 15.120 N Afg3l2 n/a
4 TRCN0000241171 ACCGGGTCCCTGTGGTTTATA pLKO_005 1913 CDS 100% 15.000 10.500 N Afg3l2 n/a
5 TRCN0000051489 GCCAAGGTCTTAAAGGATGAA pLKO.1 2083 CDS 100% 4.950 3.465 N AFG3L2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011246998.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07709 pDONR223 100% 76.7% 83.9% None (many diffs) n/a
2 ccsbBroad304_07709 pLX_304 0% 76.7% 83.9% V5 (many diffs) n/a
3 TRCN0000476795 CACCGGTGCGAAGTCTACCAGCGG pLX_317 14.1% 76.7% 83.9% V5 (many diffs) n/a
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