Construct: ORF TRCN0000476795

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017291.1_s317c1
Derived from:
ccsbBroadEn_07709
DNA Barcode:
CACCGGTGCGAAGTCTACCAGCGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AFG3L2 (10939)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000476795
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10939 AFG3L2 AFG3 like matrix AAA peptid... NM_006796.3 99.9% 100% 1389G>A;1650A>G
2 human 10939 AFG3L2 AFG3 like matrix AAA peptid... XM_011525601.3 91.5% 91.5% 1389G>A;1650A>G;1779_1780ins201
3 mouse 69597 Afg3l2 AFG3-like AAA ATPase 2 NM_027130.1 84.2% 92.4% (many diffs)
4 mouse 69597 Afg3l2 AFG3-like AAA ATPase 2 XM_011246998.1 76.7% 83.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2457
ORF length:
2391
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gcaccgctgt ttgcggctgt ggggccgggg cggctgctgg ccccgcggcc
121tacagcagct cctcgtgcct ggcggcgtgg gcccgggcga gcagccctgc ctccggacgc
181tttaccgatt tgttacaact caagcaaggg ccagcagaaa ttctcttttg acagatataa
241ttgctgctta tcaaagattc tgttctcgac ccccaaaagg atttgaaaaa tactttccta
301atggaaaaaa tggaaaaaaa gctagtgaac ctaaagaagt tatgggagag aaaaaagaat
361caaagccagc tgctaccaca cgctcttctg gaggaggagg tggtggcggt ggaaaacgag
421gtggcaagaa agatgattct cactggtggt ccaggtttca gaagggtgac attccatggg
481acgacaagga tttcaggatg ttcttcctct ggactgctct gttctggggt ggagtcatgt
541tttacttgct gctcaagaga tccgggagag aaatcacttg gaaggacttt gtcaataact
601atctttcaaa aggagtagta gacagattgg aagtcgtcaa caagcgtttt gttcgagtga
661cctttacacc aggaaaaact cctgttgatg ggcaatacgt ttggtttaat attggcagtg
721tggacacctt tgaacggaat ctggaaactt tacagcagga attgggcata gaaggagaaa
781atcgggtgcc tgttgtctac attgctgaaa gtgatggctc ttttctgctg agcatgctgc
841ctacggtgct catcatcgcc ttcttgctct acaccatcag aagagggcct gctggcattg
901gccggacagg ccgagggatg ggcggactct tcagtgtcgg agaaaccact gccaaggtct
961taaaggatga aattgatgtg aagttcaaag atgtggctgg ctgtgaggag gccaagctag
1021agatcatgga atttgtgaat ttcttgaaaa acccaaagca gtatcaagac ctaggagcaa
1081aaatcccaaa gggtgccatt ctcactggtc ctccaggcac tgggaagacg ctgctagcta
1141aggccacagc cggagaagcc aatgtcccct tcatcaccgt tagtggatct gagtttttgg
1201agatgttcgt tggtgtgggc cctgctagag tccgagactt atttgccctt gctcggaaga
1261atgccccttg catcctcttc atcgatgaaa tcgatgcggt gggaaggaag agaggaagag
1321gcaactttgg agggcagagt gagcaggaga acacactcaa ccagctgctg gtggagatgg
1381atggttttaa tacaacaaca aatgtcgtca ttttggccgg caccaatcga ccagatatcc
1441tggaccccgc gctacttagg ccggggcgtt tcgacaggca gatctttatt ggaccaccag
1501acataaaagg aagagcttct attttcaaag ttcatctccg accgctaaaa ctggacagta
1561ccctggagaa ggataaattg gcaagaaaac tggcatcttt aactccaggg ttttcaggtg
1621ctgatgttgc taatgtctgt aatgaagctg cgttgattgc tgcaaggcat ctgtcagatt
1681ccataaatca gaaacacttt gaacaggcaa ttgagcgagt gattggtggc ttagagaaga
1741aaacgcaggt tctgcagcct gaggagaaga agactgtggc ataccacgaa gcaggccatg
1801cggttgccgg ctggtatctg gagcacgcag acccgctttt aaaggtatcc atcatcccac
1861gtggcaaagg actaggttat gctcagtatt taccaaaaga acaatacctc tataccaaag
1921agcagctctt ggataggatg tgtatgactt taggtggtcg agtctctgaa gaaatcttct
1981ttggaagaat tacaactggt gctcaagatg acttgagaaa agtaactcag agtgcatatg
2041cccaaattgt tcagtttggc atgaatgaaa aggttgggca aatctccttt gacctcccac
2101gtcaggggga catggtattg gagaaaCCTT ACAGTGAAGC CACTGCAAGA TTGATAGATG
2161ATGAAGTACG AATACTTATT AATGATGCTT ATAAAAGAAC AGTAGCTCTT CTCACAGAAA
2221AGAAAGCTGA CGTGGAGAAG GTTGCTCTTC TGTTGTTAGA AAAAGAAGTA TTAGATAAGA
2281ATGATATGGT TGAACTTTTG GGCCCCAGAC CATTTGCGGA AAAATCTACC TATGAAGAAT
2341TTGTGGAAGG CACTGGCAGC TTGGATGAGG ACACCTCACT TCCAGAAGGC CTTAAGGACT
2401GGAACAAGGA GCGGGAAAAG GAGAAAGAGG AGCCCCCGGG TGAGAAAGTT GCCAACTACC
2461CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
2521TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
2581ATATATCTTG TGGAAAGGAC GACACCGGTG CGAAGTCTAC CAGCGGACGC GTTAAGTCga
2641caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)