Construct: ORF TRCN0000476795
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017291.1_s317c1
- Derived from:
- ccsbBroadEn_07709
- DNA Barcode:
- CACCGGTGCGAAGTCTACCAGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AFG3L2 (10939)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476795
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10939 | AFG3L2 | AFG3 like matrix AAA peptid... | NM_006796.3 | 99.9% | 100% | 1389G>A;1650A>G |
| 2 | human | 10939 | AFG3L2 | AFG3 like matrix AAA peptid... | XM_011525601.3 | 91.5% | 91.5% | 1389G>A;1650A>G;1779_1780ins201 |
| 3 | mouse | 69597 | Afg3l2 | AFG3-like AAA ATPase 2 | NM_027130.1 | 84.2% | 92.4% | (many diffs) |
| 4 | mouse | 69597 | Afg3l2 | AFG3-like AAA ATPase 2 | XM_011246998.1 | 76.7% | 83.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2457
- ORF length:
- 2391
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcaccgctgt ttgcggctgt ggggccgggg cggctgctgg ccccgcggcc 121 tacagcagct cctcgtgcct ggcggcgtgg gcccgggcga gcagccctgc ctccggacgc 181 tttaccgatt tgttacaact caagcaaggg ccagcagaaa ttctcttttg acagatataa 241 ttgctgctta tcaaagattc tgttctcgac ccccaaaagg atttgaaaaa tactttccta 301 atggaaaaaa tggaaaaaaa gctagtgaac ctaaagaagt tatgggagag aaaaaagaat 361 caaagccagc tgctaccaca cgctcttctg gaggaggagg tggtggcggt ggaaaacgag 421 gtggcaagaa agatgattct cactggtggt ccaggtttca gaagggtgac attccatggg 481 acgacaagga tttcaggatg ttcttcctct ggactgctct gttctggggt ggagtcatgt 541 tttacttgct gctcaagaga tccgggagag aaatcacttg gaaggacttt gtcaataact 601 atctttcaaa aggagtagta gacagattgg aagtcgtcaa caagcgtttt gttcgagtga 661 cctttacacc aggaaaaact cctgttgatg ggcaatacgt ttggtttaat attggcagtg 721 tggacacctt tgaacggaat ctggaaactt tacagcagga attgggcata gaaggagaaa 781 atcgggtgcc tgttgtctac attgctgaaa gtgatggctc ttttctgctg agcatgctgc 841 ctacggtgct catcatcgcc ttcttgctct acaccatcag aagagggcct gctggcattg 901 gccggacagg ccgagggatg ggcggactct tcagtgtcgg agaaaccact gccaaggtct 961 taaaggatga aattgatgtg aagttcaaag atgtggctgg ctgtgaggag gccaagctag 1021 agatcatgga atttgtgaat ttcttgaaaa acccaaagca gtatcaagac ctaggagcaa 1081 aaatcccaaa gggtgccatt ctcactggtc ctccaggcac tgggaagacg ctgctagcta 1141 aggccacagc cggagaagcc aatgtcccct tcatcaccgt tagtggatct gagtttttgg 1201 agatgttcgt tggtgtgggc cctgctagag tccgagactt atttgccctt gctcggaaga 1261 atgccccttg catcctcttc atcgatgaaa tcgatgcggt gggaaggaag agaggaagag 1321 gcaactttgg agggcagagt gagcaggaga acacactcaa ccagctgctg gtggagatgg 1381 atggttttaa tacaacaaca aatgtcgtca ttttggccgg caccaatcga ccagatatcc 1441 tggaccccgc gctacttagg ccggggcgtt tcgacaggca gatctttatt ggaccaccag 1501 acataaaagg aagagcttct attttcaaag ttcatctccg accgctaaaa ctggacagta 1561 ccctggagaa ggataaattg gcaagaaaac tggcatcttt aactccaggg ttttcaggtg 1621 ctgatgttgc taatgtctgt aatgaagctg cgttgattgc tgcaaggcat ctgtcagatt 1681 ccataaatca gaaacacttt gaacaggcaa ttgagcgagt gattggtggc ttagagaaga 1741 aaacgcaggt tctgcagcct gaggagaaga agactgtggc ataccacgaa gcaggccatg 1801 cggttgccgg ctggtatctg gagcacgcag acccgctttt aaaggtatcc atcatcccac 1861 gtggcaaagg actaggttat gctcagtatt taccaaaaga acaatacctc tataccaaag 1921 agcagctctt ggataggatg tgtatgactt taggtggtcg agtctctgaa gaaatcttct 1981 ttggaagaat tacaactggt gctcaagatg acttgagaaa agtaactcag agtgcatatg 2041 cccaaattgt tcagtttggc atgaatgaaa aggttgggca aatctccttt gacctcccac 2101 gtcaggggga catggtattg gagaaaCCTT ACAGTGAAGC CACTGCAAGA TTGATAGATG 2161 ATGAAGTACG AATACTTATT AATGATGCTT ATAAAAGAAC AGTAGCTCTT CTCACAGAAA 2221 AGAAAGCTGA CGTGGAGAAG GTTGCTCTTC TGTTGTTAGA AAAAGAAGTA TTAGATAAGA 2281 ATGATATGGT TGAACTTTTG GGCCCCAGAC CATTTGCGGA AAAATCTACC TATGAAGAAT 2341 TTGTGGAAGG CACTGGCAGC TTGGATGAGG ACACCTCACT TCCAGAAGGC CTTAAGGACT 2401 GGAACAAGGA GCGGGAAAAG GAGAAAGAGG AGCCCCCGGG TGAGAAAGTT GCCAACTACC 2461 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 2521 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2581 ATATATCTTG TGGAAAGGAC GACACCGGTG CGAAGTCTAC CAGCGGACGC GTTAAGTCga 2641 caatcaacct ctggattaca aaatttgtga aagatt