Transcript: Mouse XM_011249666.2

PREDICTED: Mus musculus phosphodiesterase 7A (Pde7a), transcript variant X4, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Pde7a (18583)
Length:
4409
CDS:
427..1437

Additional Resources:

NCBI RefSeq record:
XM_011249666.2
NBCI Gene record:
Pde7a (18583)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_011249666.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000114947 CCAGGTGTTAATCAGCCGTTT pLKO.1 757 CDS 100% 4.050 5.670 N Pde7a n/a
2 TRCN0000114948 GCTTTGATATTAGCCACGGAT pLKO.1 934 CDS 100% 2.640 3.696 N Pde7a n/a
3 TRCN0000313089 ATCGTCAGACTGAGTCTATTG pLKO_005 1196 CDS 100% 10.800 8.640 N Pde7a n/a
4 TRCN0000435894 GGCCATGCACTGTTACTTAAA pLKO_005 654 CDS 100% 13.200 9.240 N PDE7A n/a
5 TRCN0000313088 TTTGTAGTTCTCTAGACATTT pLKO_005 197 5UTR 100% 13.200 9.240 N Pde7a n/a
6 TRCN0000428622 TTTGGGTGTGAGTCCACTTTG pLKO_005 1173 CDS 100% 10.800 7.560 N PDE7A n/a
7 TRCN0000114950 CACAGTTACTAACTCAGGAAA pLKO.1 1403 CDS 100% 4.950 3.465 N Pde7a n/a
8 TRCN0000114946 GCCAGCATTATCTATCAAGAT pLKO.1 1541 3UTR 100% 4.950 3.465 N Pde7a n/a
9 TRCN0000312054 GCCAGCATTATCTATCAAGAT pLKO_005 1541 3UTR 100% 4.950 3.465 N Pde7a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011249666.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01161 pDONR223 100% 67.1% 69.9% None (many diffs) n/a
2 ccsbBroad304_01161 pLX_304 0% 67.1% 69.9% V5 (many diffs) n/a
3 TRCN0000476402 CGCGCCTGTCTGGTGGAAGGCGTC pLX_317 28.9% 67.1% 69.9% V5 (many diffs) n/a
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