Construct: ORF TRCN0000476402
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013740.1_s317c1
- Derived from:
- ccsbBroadEn_01161
- DNA Barcode:
- CGCGCCTGTCTGGTGGAAGGCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE7A (5150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476402
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5150 | PDE7A | phosphodiesterase 7A | NM_002603.4 | 100% | 100% | |
| 2 | human | 5150 | PDE7A | phosphodiesterase 7A | XM_011517540.3 | 100% | 100% | |
| 3 | human | 5150 | PDE7A | phosphodiesterase 7A | XM_017013538.2 | 95.5% | 94.5% | (many diffs) |
| 4 | human | 5150 | PDE7A | phosphodiesterase 7A | NM_001242318.3 | 93.1% | 89.6% | (many diffs) |
| 5 | mouse | 18583 | Pde7a | phosphodiesterase 7A | NM_008802.3 | 90.3% | 94% | (many diffs) |
| 6 | mouse | 18583 | Pde7a | phosphodiesterase 7A | XM_006535405.3 | 84.3% | 86.4% | (many diffs) |
| 7 | mouse | 18583 | Pde7a | phosphodiesterase 7A | NM_001122759.2 | 84.1% | 84.1% | (many diffs) |
| 8 | mouse | 18583 | Pde7a | phosphodiesterase 7A | XM_006535406.3 | 79.1% | 78.4% | (many diffs) |
| 9 | mouse | 18583 | Pde7a | phosphodiesterase 7A | XM_011249666.2 | 67.1% | 69.9% | (many diffs) |
| 10 | mouse | 18583 | Pde7a | phosphodiesterase 7A | XM_006535408.3 | 57.6% | 60.3% | (many diffs) |
| 11 | mouse | 18583 | Pde7a | phosphodiesterase 7A | XR_389588.3 | 57.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1437
- ORF length:
- 1368
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaattaca ttgatctggt gtctggcctt ggttcttatc aagtggatca 121 cctctaagag gcgtggagct atttcctatg acagttctga tcagactgca ttatacattc 181 gtatgctagg agatgtacgt gtaaggagcc gagcaggatt tgaatcagaa agaagaggtt 241 ctcacccata tattgatttt cgtattttcc actctcaatc tgaaattgaa gtgtctgtct 301 ctgcaaggaa tatcagaagg ctactaagtt tccagcgata tcttagatct tcacgctttt 361 ttcgtggtac tgcggtttca aattccctaa acattttaga tgatgattat aatggacaag 421 ccaagtgtat gctggaaaaa gttggaaatt ggaattttga tatctttcta tttgatagac 481 taacaaatgg aaatagtcta gtaagcttaa cctttcattt atttagtctt catggattaa 541 ttgagtactt ccatttagat atgatgaaac ttcgtagatt tttagttatg attcaagaag 601 attaccacag tcaaaatcct taccataacg cagtccacgc tgcggatgtt actcaggcca 661 tgcactgtta cttaaaggaa cctaagcttg ccaattctgt aactccttgg gatatcttgc 721 tgagcttaat tgcagctgcc actcatgatc tggatcatcc aggtgttaat caacctttcc 781 ttattaaaac taaccattac ttggcaactt tatacaagaa tacctcagta ctggaaaatc 841 accactggag atctgcagtg ggcttattga gagaatcagg cttattctca catctgccat 901 tagaaagcag gcaacaaatg gagacacaga taggtgctct gatactagcc acagacatca 961 gtcgccagaa tgagtatctg tctttgttta ggtcccattt ggatagaggt gatttatgcc 1021 tagaagacac cagacacaga catttGGTTT TACAGATGGC TTTGAAATGT GCTGATATTT 1081 GTAACCCATG TCGGACGTGG GAATTAAGCA AGCAGTGGAG TGAAAAAGTA ACGGAGGAAT 1141 TCTTCCATCA AGGAGATATA GAAAAAAAAT ATCATTTGGG TGTGAGTCCA CTTTGCGATC 1201 GTCACACTGA ATCTATTGCC AACATCCAGA TTGGTTTTAT GACTTACCTA GTGGAGCCTT 1261 TATTTACAGA ATGGGCCAGG TTTTCCAATA CAAGGCTATC CCAGACAATG CTTGGACACG 1321 TGGGGCTGAA TAAAGCCAGC TGGAAGGGAC TGCAGAGAGA ACAGTCGAGC AGTGAGGACA 1381 CTGATGCTGC ATTTGAGTTG AACTCACAGT TATTACCTCA GGAAAATCGG TTATCATTGC 1441 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1501 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1561 ATATATCTTG TGGAAAGGAC GACGCGCCTG TCTGGTGGAA GGCGTCACGC GTTAAGTCga 1621 caatcaacct ctggattaca aaatttgtga aagatt