Transcript: Human XM_011509342.2

PREDICTED: Homo sapiens ral guanine nucleotide dissociation stimulator like 1 (RGL1), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RGL1 (23179)
Length:
2377
CDS:
441..2303

Additional Resources:

NCBI RefSeq record:
XM_011509342.2
NBCI Gene record:
RGL1 (23179)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011509342.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000048064 CCACCATCTCTCAGTTTAATA pLKO.1 1378 CDS 100% 15.000 21.000 N RGL1 n/a
2 TRCN0000310593 CCACCATCTCTCAGTTTAATA pLKO_005 1378 CDS 100% 15.000 21.000 N RGL1 n/a
3 TRCN0000303950 TAATTCCATCTATCGGTTAAA pLKO_005 1556 CDS 100% 13.200 18.480 N RGL1 n/a
4 TRCN0000303949 TGGAAGATCATGTTCGAATAT pLKO_005 529 CDS 100% 13.200 18.480 N RGL1 n/a
5 TRCN0000303956 GACAATGACTTTACCTATATC pLKO_005 804 CDS 100% 13.200 9.240 N RGL1 n/a
6 TRCN0000048067 CCTGTAAGATCAGGACCATAA pLKO.1 733 CDS 100% 10.800 7.560 N RGL1 n/a
7 TRCN0000048066 GCACAACTCTTCAAGAAAGTA pLKO.1 1278 CDS 100% 5.625 3.938 N RGL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011509342.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02728 pDONR223 100% 77.1% 76.9% None 1847delA;1860_1861ins550 n/a
2 ccsbBroad304_02728 pLX_304 0% 77.1% 76.9% V5 1847delA;1860_1861ins550 n/a
3 TRCN0000477017 GGCACACCGTGGTACATCTTCAGG pLX_317 16.4% 77.1% 76.9% V5 1847delA;1860_1861ins550 n/a
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