Construct: ORF ccsbBroad304_02728
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011653.1_s304c1, BRDN0000462157
- Derived from:
- ccsbBroadEn_02728
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGL1 (23179)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02728
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23179 | RGL1 | ral guanine nucleotide diss... | NM_015149.5 | 100% | 100% | |
| 2 | human | 23179 | RGL1 | ral guanine nucleotide diss... | XM_011509339.3 | 100% | 100% | |
| 3 | human | 23179 | RGL1 | ral guanine nucleotide diss... | NM_001297669.2 | 95.3% | 95.3% | 131_132ins111 |
| 4 | human | 23179 | RGL1 | ral guanine nucleotide diss... | NM_001297670.2 | 95.3% | 95.3% | 131_132ins111 |
| 5 | human | 23179 | RGL1 | ral guanine nucleotide diss... | NM_001297671.2 | 95% | 94.6% | (many diffs) |
| 6 | human | 23179 | RGL1 | ral guanine nucleotide diss... | NM_001297672.2 | 91.4% | 91% | (many diffs) |
| 7 | human | 23179 | RGL1 | ral guanine nucleotide diss... | XM_011509341.1 | 89.4% | 89.4% | 1853_1854ins255 |
| 8 | human | 23179 | RGL1 | ral guanine nucleotide diss... | XM_017000756.2 | 84.4% | 84% | (many diffs) |
| 9 | human | 23179 | RGL1 | ral guanine nucleotide diss... | XM_011509342.2 | 77.1% | 76.9% | 1847delA;1860_1861ins550 |
| 10 | mouse | 19731 | Rgl1 | ral guanine nucleotide diss... | NM_001346117.1 | 89.8% | 94.2% | (many diffs) |
| 11 | mouse | 19731 | Rgl1 | ral guanine nucleotide diss... | NM_001346118.1 | 89.8% | 94.2% | (many diffs) |
| 12 | mouse | 19731 | Rgl1 | ral guanine nucleotide diss... | NM_016846.4 | 85.5% | 90% | (many diffs) |
| 13 | mouse | 19731 | Rgl1 | ral guanine nucleotide diss... | NM_001346119.1 | 85.3% | 89.6% | (many diffs) |
| 14 | mouse | 19731 | Rgl1 | ral guanine nucleotide diss... | XM_006529266.4 | 85.3% | 89.6% | (many diffs) |
| 15 | mouse | 19731 | Rgl1 | ral guanine nucleotide diss... | XM_006529268.4 | 80.5% | 85.3% | (many diffs) |
| 16 | mouse | 19731 | Rgl1 | ral guanine nucleotide diss... | XM_006529267.4 | 65.7% | 69.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2478
- ORF length:
- 2409
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggtgaaa cctgtgggag aacctactca agaggtgtct aaattcaaac 121 tttccaccaa ggtagaaagc acgggacact ggctggtgga agatcatgtt cgaatatggg 181 aagttttaaa gacagaggag agctcgattc aggactgggg tgaagaggta gaggaaggag 241 ctgtttacca tgtcaccctc aaaagagtcc agattcaaca ggctgccaat aaaggagcaa 301 gatggctagg ggttgaaggg gaccagctgc ctccaggaca cacagtcagt caatatgaaa 361 cctgtaagat caggaccata aaagctggca ccttggagaa gcttgtggag aacctgctga 421 cagcttttgg ggacaatgac tttacctata tcagcatctt tctttcaacg tacagaggct 481 ttgcctccac taaagaagtg ctggaactac tgctggacag gtatggaaac ctgacaagcc 541 caaactgtga agaagatgga agccaaagtt catcagagtc caaaatggtg atcaggaatg 601 caatcgcttc catactaagg gcctggcttg accagtgtgc agaagacttc cgagagcccc 661 ctcacttccc ttgcttacag aaactgctgg attatctcac acggatgatg ccgggctctg 721 acccagaaag aagagcacaa aatcttcttg agcagtttca gaagcaagaa gtggaaactg 781 acaatgggct tcccaacacg atctccttca gcctggaaga ggaagaggaa ctggagggtg 841 gagagtcagc agaattcacg tgcttctcag aagatctcgt ggcagagcag ctgacctaca 901 tggatgcaca actcttcaag aaagtagtgc ctcaccactg cctgggctgc atttggtctc 961 gaagggataa gaaggaaaac aaacatttgg ctcctacgat ccgtgccacc atctctcagt 1021 ttaataccct caccaaatgt gttgtcagca ccatcctggg gggcaaagaa ctcaaaactc 1081 agcagagagc caaaatcatt gagaagtgga tcaacatcgc tcatgaatgt agactcctga 1141 agaatttttc ctccttgagg gccatcgttt cggcactgca gtctaattcc atctatcggt 1201 taaaaaagac ttgggctgcc gtcccaaggg accgaatgct gatgtttgaa gaactttcag 1261 atatcttctc agaccataat aaccatttga ccagccgaga actactgatg aaggaaggaa 1321 cctcaaaatt tgcaaacctg gacagcagtg tgaaagaaaa ccagaagcgt acccagaggc 1381 ggctgcagct ccagaaggac atgggtgtga tgcagggaac tgtgccctac ctgggcacct 1441 tcctgactga cctgaccatg cttgacactg cccttcagga ctacatcgag ggtggactga 1501 taaactttga gaaaaggaga agggaatttg aagtgattgc ccagataaag ctcttacagt 1561 ctgcctgcaa cagctattgc atgaccccag accaaaagtt catccagtgg ttccagaggc 1621 agcagctcct gacagaggag gagagctatg ccctgtcatg tgagattgaa gcagctgctg 1681 acgccagcac cacctcgccc aagcctcgga agagcatggt gaagagactc agcctactgt 1741 ttctagggtc tgacatgatc accagtccca ctcccaccaa agagcagccc aagtccactg 1801 ccagcgggag ctctggtgaa agcatggact ctgtcagcgt gtcatcctgc gagtcgaacc 1861 actcagaggc tgaggagggc tccattactc ccatggacac ccctgatgag cctcaaaaaa 1921 agctctctga gtcctcctca tcctgttctt ctatccattc catggacaca aattcctcag 1981 ggatgtcttc cttaatcaac cccctctcct cccctccgtc ctgcaacaac aaccccaaaa 2041 tccacaagcg ctctgtctcg gtgacgtcca ttacctcgac tgtgctgcct cctgtttaca 2101 accaacagaa tgaagacacc tgcataatcc gcatcagtgt ggaagacaat aacggcaaca 2161 tgtacaagag catcatgttg acgagccagg ataaaacccc cgctgtgatc cagagagcca 2221 tgctgaagca caatctggac tcagaccccg ccgaggagta cgagctggtg caggtcatct 2281 cggaggacaa agaacttgtg attccagact cagcaaatgt cttttatgcc atgaacagcc 2341 aagtgaactt tgacttcatt ttgcgcaaaa agaactccat ggaagaacaa gtgaaactgc 2401 gtagccggac cagcttgacg ttgcccagga cagctaaacg gggctgctgg agtaacagac 2461 acagcaaaat caccctcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 2521 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 2581 agtcgacaat caacctctgg atta