Construct: ORF ccsbBroad304_02728

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011653.1_s304c1, BRDN0000462157
Derived from:
ccsbBroadEn_02728
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RGL1 (23179)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02728
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23179 RGL1 ral guanine nucleotide diss... NM_015149.5 100% 100%
2 human 23179 RGL1 ral guanine nucleotide diss... XM_011509339.3 100% 100%
3 human 23179 RGL1 ral guanine nucleotide diss... NM_001297669.2 95.3% 95.3% 131_132ins111
4 human 23179 RGL1 ral guanine nucleotide diss... NM_001297670.2 95.3% 95.3% 131_132ins111
5 human 23179 RGL1 ral guanine nucleotide diss... NM_001297671.2 95% 94.6% (many diffs)
6 human 23179 RGL1 ral guanine nucleotide diss... NM_001297672.2 91.4% 91% (many diffs)
7 human 23179 RGL1 ral guanine nucleotide diss... XM_011509341.1 89.4% 89.4% 1853_1854ins255
8 human 23179 RGL1 ral guanine nucleotide diss... XM_017000756.2 84.4% 84% (many diffs)
9 human 23179 RGL1 ral guanine nucleotide diss... XM_011509342.2 77.1% 76.9% 1847delA;1860_1861ins550
10 mouse 19731 Rgl1 ral guanine nucleotide diss... NM_001346117.1 89.8% 94.2% (many diffs)
11 mouse 19731 Rgl1 ral guanine nucleotide diss... NM_001346118.1 89.8% 94.2% (many diffs)
12 mouse 19731 Rgl1 ral guanine nucleotide diss... NM_016846.4 85.5% 90% (many diffs)
13 mouse 19731 Rgl1 ral guanine nucleotide diss... NM_001346119.1 85.3% 89.6% (many diffs)
14 mouse 19731 Rgl1 ral guanine nucleotide diss... XM_006529266.4 85.3% 89.6% (many diffs)
15 mouse 19731 Rgl1 ral guanine nucleotide diss... XM_006529268.4 80.5% 85.3% (many diffs)
16 mouse 19731 Rgl1 ral guanine nucleotide diss... XM_006529267.4 65.7% 69.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2478
ORF length:
2409
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaggtgaaa cctgtgggag aacctactca agaggtgtct aaattcaaac
121tttccaccaa ggtagaaagc acgggacact ggctggtgga agatcatgtt cgaatatggg
181aagttttaaa gacagaggag agctcgattc aggactgggg tgaagaggta gaggaaggag
241ctgtttacca tgtcaccctc aaaagagtcc agattcaaca ggctgccaat aaaggagcaa
301gatggctagg ggttgaaggg gaccagctgc ctccaggaca cacagtcagt caatatgaaa
361cctgtaagat caggaccata aaagctggca ccttggagaa gcttgtggag aacctgctga
421cagcttttgg ggacaatgac tttacctata tcagcatctt tctttcaacg tacagaggct
481ttgcctccac taaagaagtg ctggaactac tgctggacag gtatggaaac ctgacaagcc
541caaactgtga agaagatgga agccaaagtt catcagagtc caaaatggtg atcaggaatg
601caatcgcttc catactaagg gcctggcttg accagtgtgc agaagacttc cgagagcccc
661ctcacttccc ttgcttacag aaactgctgg attatctcac acggatgatg ccgggctctg
721acccagaaag aagagcacaa aatcttcttg agcagtttca gaagcaagaa gtggaaactg
781acaatgggct tcccaacacg atctccttca gcctggaaga ggaagaggaa ctggagggtg
841gagagtcagc agaattcacg tgcttctcag aagatctcgt ggcagagcag ctgacctaca
901tggatgcaca actcttcaag aaagtagtgc ctcaccactg cctgggctgc atttggtctc
961gaagggataa gaaggaaaac aaacatttgg ctcctacgat ccgtgccacc atctctcagt
1021ttaataccct caccaaatgt gttgtcagca ccatcctggg gggcaaagaa ctcaaaactc
1081agcagagagc caaaatcatt gagaagtgga tcaacatcgc tcatgaatgt agactcctga
1141agaatttttc ctccttgagg gccatcgttt cggcactgca gtctaattcc atctatcggt
1201taaaaaagac ttgggctgcc gtcccaaggg accgaatgct gatgtttgaa gaactttcag
1261atatcttctc agaccataat aaccatttga ccagccgaga actactgatg aaggaaggaa
1321cctcaaaatt tgcaaacctg gacagcagtg tgaaagaaaa ccagaagcgt acccagaggc
1381ggctgcagct ccagaaggac atgggtgtga tgcagggaac tgtgccctac ctgggcacct
1441tcctgactga cctgaccatg cttgacactg cccttcagga ctacatcgag ggtggactga
1501taaactttga gaaaaggaga agggaatttg aagtgattgc ccagataaag ctcttacagt
1561ctgcctgcaa cagctattgc atgaccccag accaaaagtt catccagtgg ttccagaggc
1621agcagctcct gacagaggag gagagctatg ccctgtcatg tgagattgaa gcagctgctg
1681acgccagcac cacctcgccc aagcctcgga agagcatggt gaagagactc agcctactgt
1741ttctagggtc tgacatgatc accagtccca ctcccaccaa agagcagccc aagtccactg
1801ccagcgggag ctctggtgaa agcatggact ctgtcagcgt gtcatcctgc gagtcgaacc
1861actcagaggc tgaggagggc tccattactc ccatggacac ccctgatgag cctcaaaaaa
1921agctctctga gtcctcctca tcctgttctt ctatccattc catggacaca aattcctcag
1981ggatgtcttc cttaatcaac cccctctcct cccctccgtc ctgcaacaac aaccccaaaa
2041tccacaagcg ctctgtctcg gtgacgtcca ttacctcgac tgtgctgcct cctgtttaca
2101accaacagaa tgaagacacc tgcataatcc gcatcagtgt ggaagacaat aacggcaaca
2161tgtacaagag catcatgttg acgagccagg ataaaacccc cgctgtgatc cagagagcca
2221tgctgaagca caatctggac tcagaccccg ccgaggagta cgagctggtg caggtcatct
2281cggaggacaa agaacttgtg attccagact cagcaaatgt cttttatgcc atgaacagcc
2341aagtgaactt tgacttcatt ttgcgcaaaa agaactccat ggaagaacaa gtgaaactgc
2401gtagccggac cagcttgacg ttgcccagga cagctaaacg gggctgctgg agtaacagac
2461acagcaaaat caccctcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta
2521accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
2581agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)