Transcript: Human XM_011521122.3

PREDICTED: Homo sapiens transmembrane O-mannosyltransferase targeting cadherins 4 (TMTC4), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TMTC4 (84899)
Length:
4512
CDS:
900..3356

Additional Resources:

NCBI RefSeq record:
XM_011521122.3
NBCI Gene record:
TMTC4 (84899)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011521122.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000151438 GCTTTATTCCTCAAGGCAATT pLKO.1 3147 CDS 100% 10.800 7.560 N TMTC4 n/a
2 TRCN0000178703 CCTGACTTTCAGGATTAACTA pLKO.1 1223 CDS 100% 5.625 3.938 N Tmtc4 n/a
3 TRCN0000293095 CCTGACTTTCAGGATTAACTA pLKO_005 1223 CDS 100% 5.625 3.938 N Tmtc4 n/a
4 TRCN0000152153 CTCTGACTTGTGCAATTTCAA pLKO.1 3990 3UTR 100% 5.625 3.938 N TMTC4 n/a
5 TRCN0000151358 GCTAAGGTTCACTACAACATT pLKO.1 2574 CDS 100% 5.625 3.938 N TMTC4 n/a
6 TRCN0000152346 CACCAGCAATATACTCTTGAA pLKO.1 3508 3UTR 100% 4.950 3.465 N TMTC4 n/a
7 TRCN0000152485 CCTGAGCAAACATACCAAGAA pLKO.1 2423 CDS 100% 4.950 3.465 N TMTC4 n/a
8 TRCN0000153751 CGTCTGTATGCAGATCTCAAT pLKO.1 2904 CDS 100% 4.950 3.465 N TMTC4 n/a
9 TRCN0000151284 CTGAGAAGAAAGCTAGAACTA pLKO.1 3315 CDS 100% 4.950 3.465 N TMTC4 n/a
10 TRCN0000151611 GCTGGGAATCTTATTCATCAA pLKO.1 2468 CDS 100% 4.950 3.465 N TMTC4 n/a
11 TRCN0000151556 GCAATTTCAATACACAGGAGA pLKO.1 4001 3UTR 100% 2.640 1.848 N TMTC4 n/a
12 TRCN0000198290 GCTAAGTTAGTAGTGGGATTT pLKO.1 1020 CDS 100% 10.800 15.120 N Tmtc4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011521122.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14318 pDONR223 100% 47.4% 45% None (many diffs) n/a
2 ccsbBroad304_14318 pLX_304 0% 47.4% 45% V5 (many diffs) n/a
3 TRCN0000478510 TTTTGAGCACGAAAAGTAATTTTG pLX_317 25.8% 47.4% 45% V5 (many diffs) n/a
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