Construct: ORF TRCN0000478510

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003588.2_s317c1
Derived from:
ccsbBroadEn_14318
DNA Barcode:
TTTTGAGCACGAAAAGTAATTTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMTC4 (84899)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478510
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84899 TMTC4 transmembrane O-mannosyltra... XM_017020798.1 73.9% 69.5% (many diffs)
2 human 84899 TMTC4 transmembrane O-mannosyltra... XM_017020799.2 73.9% 69.5% (many diffs)
3 human 84899 TMTC4 transmembrane O-mannosyltra... XM_017020797.2 66.6% 62.7% (many diffs)
4 human 84899 TMTC4 transmembrane O-mannosyltra... NM_001286453.3 61.5% 58.1% (many diffs)
5 human 84899 TMTC4 transmembrane O-mannosyltra... XM_011521125.1 55.2% 54.9% (many diffs)
6 human 84899 TMTC4 transmembrane O-mannosyltra... NM_001079669.4 52.3% 49.6% (many diffs)
7 human 84899 TMTC4 transmembrane O-mannosyltra... NM_001350572.2 52.3% 49.6% (many diffs)
8 human 84899 TMTC4 transmembrane O-mannosyltra... NM_001350577.2 52.1% 49.3% (many diffs)
9 human 84899 TMTC4 transmembrane O-mannosyltra... NM_032813.5 51% 48.3% (many diffs)
10 human 84899 TMTC4 transmembrane O-mannosyltra... NM_001350576.2 50.8% 48.1% (many diffs)
11 human 84899 TMTC4 transmembrane O-mannosyltra... NM_001350574.2 48.5% 46% (many diffs)
12 human 84899 TMTC4 transmembrane O-mannosyltra... XM_011521123.2 48.5% 46% (many diffs)
13 human 84899 TMTC4 transmembrane O-mannosyltra... NM_001350571.2 47.4% 45% (many diffs)
14 human 84899 TMTC4 transmembrane O-mannosyltra... XM_011521122.3 47.4% 45% (many diffs)
15 human 84899 TMTC4 transmembrane O-mannosyltra... NR_146794.2 30.5% (many diffs)
16 human 84899 TMTC4 transmembrane O-mannosyltra... XM_017020800.1 24.2% 23.6% (many diffs)
17 mouse 70551 Tmtc4 transmembrane and tetratric... XM_017316184.1 64.4% 62.8% (many diffs)
18 mouse 70551 Tmtc4 transmembrane and tetratric... XM_017316185.1 57.3% 56.1% (many diffs)
19 mouse 70551 Tmtc4 transmembrane and tetratric... XM_017316183.1 54.4% 53.2% (many diffs)
20 mouse 70551 Tmtc4 transmembrane and tetratric... XM_006519511.3 45.7% 44.9% (many diffs)
21 mouse 70551 Tmtc4 transmembrane and tetratric... XM_017316182.1 45.7% 44.9% (many diffs)
22 mouse 70551 Tmtc4 transmembrane and tetratric... NM_028651.2 45.6% 44.8% (many diffs)
23 mouse 70551 Tmtc4 transmembrane and tetratric... XM_006519509.3 45.6% 44.8% (many diffs)
24 mouse 70551 Tmtc4 transmembrane and tetratric... XM_006519510.3 45.6% 44.8% (many diffs)
25 mouse 70551 Tmtc4 transmembrane and tetratric... XM_006519512.1 45.6% 44.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1251
ORF length:
1185
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gtgcaaagan caagggatca ctgtgctggg tttaaatgcg gtatttgaca
121tcttggtgat aggcaaattc aatgttctgg aaattgtcca gaaggtacta cataaggaca
181agtcattaga gaatctcggc atgctcagga acgggggcct cctcttcaga atgaccctgc
241tcacctctgg aggggctggg atgctctacg tgcgctggag gatcatgggc acgggcccgc
301cggccttcac cgaggtggac aacccggcct cctttgctga cagcatgctg gtgagggccg
361taaactacaa ttactactat tcattgaatg cctggctgct gctgtgtccc tggtggctgt
421gttttgattg gtcaatgggc tgcatccccc tcattaagtc catcagcgac tggagggtaa
481ttgcacttgc agcactctgg ttctgcctaa ttggcctgat atgccaagcc ctgtgctctg
541aagacggcca caagagaagg atccttactc tgggcctggg atttctcgtt atcccatttc
601tccccgcgag taacctgttc ttccgagtgg gcttcgtggt cgcggagcgt gtcctctacc
661tccccagcgt tgggtactgt gtgctgctga cttttggatt cggagccctg agcaaacata
721ccaagaaaaa gaaactcatt gccgctgtcg tgctgggaat cttattcatc aacacgctga
781gatgtgtgct gcgcagcggc gagtggcgga gtgaggaaca gcttttcaga agtgctctgt
841ctgtgtgtcc cctcaatgct aaggttcact acaacattgg caaaaacctg gctgataaag
901gcaaccagac agctgccatc agatactacc gggaagctgt aagattaaat CCCAAGTATG
961TTCATGCCAT GAATAATCTT GGAAATATCT TAAAAGAAAG GAATGAGCTA CAGGAAGCTG
1021AGGAGCTGCT GTCTTTGGCT GTTCAAATAC AGCCAGACTT TGCCGCTGCG TGGATGAATC
1081TAGGCATAGT GCAGAATAGC CTGAAACGGT TTGAAGCAGC AGAGCAAAGT TACCGGACAG
1141CAATTAAACA CAGAAGGAAA TACCCAGACT GTTACTACAA CCTCGGGCGT CTGGTAAGCG
1201CGGGGTGCCC TGTGCCTGTG GAAGGAAAGA TGGGTTATTT TTCTTATTTA TACCCAACTT
1261TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1321CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1381CTTGTGGAAA GGACGATTTT GAGCACGAAA AGTAATTTTG ACGCGTTAAG TCgacaatca
1441acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)