Transcript: Human XM_011522513.2

PREDICTED: Homo sapiens poly(A)-specific ribonuclease (PARN), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PARN (5073)
Length:
2263
CDS:
246..1820

Additional Resources:

NCBI RefSeq record:
XM_011522513.2
NBCI Gene record:
PARN (5073)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011522513.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000049746 CCTATGTATCTCCTAACACTT pLKO.1 541 CDS 100% 4.950 6.930 N PARN n/a
2 TRCN0000049744 CCCAGACTCTTGGATACTAAA pLKO.1 1020 CDS 100% 13.200 9.240 N PARN n/a
3 TRCN0000049745 GCCTTTGGTAACATTCAGATA pLKO.1 1461 CDS 100% 4.950 3.465 N PARN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011522513.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01146 pDONR223 100% 79.5% 77.7% None (many diffs) n/a
2 ccsbBroad304_01146 pLX_304 0% 79.5% 77.7% V5 (many diffs) n/a
3 TRCN0000478950 AAAGAAAACACCACATCATTTCTC pLX_317 20.5% 79.5% 77.7% V5 (many diffs) n/a
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