Construct: ORF TRCN0000478950
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004077.2_s317c1
- Derived from:
- ccsbBroadEn_01146
- DNA Barcode:
- AAAGAAAACACCACATCATTTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PARN (5073)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478950
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5073 | PARN | poly(A)-specific ribonuclease | NM_002582.4 | 100% | 100% | |
| 2 | human | 5073 | PARN | poly(A)-specific ribonuclease | NM_001242992.2 | 92.8% | 92.8% | 156_157ins138 |
| 3 | human | 5073 | PARN | poly(A)-specific ribonuclease | NM_001134477.3 | 90.4% | 90.4% | 0_1ins183 |
| 4 | human | 5073 | PARN | poly(A)-specific ribonuclease | XM_011522510.3 | 89% | 87.1% | (many diffs) |
| 5 | human | 5073 | PARN | poly(A)-specific ribonuclease | XM_011522511.2 | 88.5% | 87.9% | (many diffs) |
| 6 | human | 5073 | PARN | poly(A)-specific ribonuclease | XM_017023258.2 | 85% | 83.1% | (many diffs) |
| 7 | human | 5073 | PARN | poly(A)-specific ribonuclease | XM_011522513.2 | 79.5% | 77.7% | (many diffs) |
| 8 | human | 5073 | PARN | poly(A)-specific ribonuclease | XR_001751906.2 | 73.1% | 1_161del;1078_1079ins44;1682_1683ins352 | |
| 9 | human | 5073 | PARN | poly(A)-specific ribonuclease | XM_011522514.2 | 66.3% | 66% | (many diffs) |
| 10 | human | 5073 | PARN | poly(A)-specific ribonuclease | XR_001751907.2 | 61.8% | (many diffs) | |
| 11 | human | 5073 | PARN | poly(A)-specific ribonuclease | XM_017023259.2 | 45.7% | 38.8% | (many diffs) |
| 12 | human | 5073 | PARN | poly(A)-specific ribonuclease | XM_017023260.1 | 45.7% | 38.8% | (many diffs) |
| 13 | human | 5073 | PARN | poly(A)-specific ribonuclease | XM_024450292.1 | 45.7% | 38.8% | (many diffs) |
| 14 | mouse | 74108 | Parn | poly(A)-specific ribonuclea... | NM_028761.3 | 84.7% | 86.5% | (many diffs) |
| 15 | mouse | 74108 | Parn | poly(A)-specific ribonuclea... | XM_011246024.2 | 82.1% | 84% | (many diffs) |
| 16 | mouse | 74108 | Parn | poly(A)-specific ribonuclea... | XM_011246023.2 | 82% | 83.5% | (many diffs) |
| 17 | mouse | 74108 | Parn | poly(A)-specific ribonuclea... | XM_006522652.2 | 75.8% | 77.6% | (many diffs) |
| 18 | mouse | 74108 | Parn | poly(A)-specific ribonuclea... | XM_017317144.1 | 75.8% | 77.6% | (many diffs) |
| 19 | mouse | 74108 | Parn | poly(A)-specific ribonuclea... | XM_017317145.1 | 73.2% | 75.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1983
- ORF length:
- 1917
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gataatcagg agcaatttta agagtaatct tcacaaagtg taccaggcca 121 tagaggaggc cgacttcttc gccatcgatg gggagttttc aggaatcagt gatggacctt 181 cagtctctgc attaacaaat ggttttgaca ctccagaaga gaggtatcag aagcttaaaa 241 agcattccat ggactttttg ctatttcagt ttggcctttg cacttttaag tatgactaca 301 cagattcaaa gtatataacg aagtcattta acttctatgt tttcccgaaa cccttcaata 361 gatcctcacc agatgtcaaa tttgtttgtc agagctccag cattgacttt ctagcaagcc 421 agggatttga ttttaataaa gtttttcgaa atggaattcc atatttaaat caggaagaag 481 aaagacagtt aagagagcag tatgatgaaa aacgttcaca ggcgaatggt gcaggagctc 541 tgtcctatgt atctcctaac acttcaaaat gtcctgtcac gattcctgag gatcaaaaga 601 agtttattga ccaagtggta gagaaaatag aggatttatt acaaagtgaa gaaaacaaga 661 acttggattt agagccatgt accgggttcc aaagaaaact aatttatcag actttgagct 721 ggaagtatcc gaaaggcatt catgttgaga ctttagaaac tgaaaagaag gagcgatata 781 tagttatcag caaagtagat gaagaagaac gcaaaagaag agagcagcag aaacatgcca 841 aagaacagga ggagctgaat gatgctgtgg gattttctag agtcattcac gccattgcta 901 attcgggaaa acttgttatt ggacacaata tgctcttgga cgtcatgcac acagttcatc 961 agttctactg ccctctgcct gcggacttaa gtgagtttaa agagatgaca acatgtgttt 1021 tccccagact cttggatact aaattgatgg ccagcacaca accttttaag gatatcatta 1081 acaacacatc ccttgcggaa ttggaaaagc ggttaaaaga gacacctttc aaccctccta 1141 aagttgaaag tgccgaaggt tttccaagtt atgacacagc ctctgaacaa ctccacgagg 1201 caggctacga tgcctacatc acagggctgt gcttcatctc catggccaat tacctaggtt 1261 cttttctcag ccctccaaaa attcatgtgt ctgccagatc aaaactcatt gaaccttttt 1321 ttaacaagtt atttcttatg agggtcatgg atatccccta tctaaacttg gaaggaccag 1381 acttgcagcc taaacgtgat catgttctcc atgtgacatt ccccaaagaa tggaaaacca 1441 gcgaccttta ccagcttttc agtgcctttg gtaacattca gatatcctgg attgatgaca 1501 catcagcatt tgtttccctt agccagcccg agcaagtaaa gattgctgtc aataccagca 1561 aatatgcaga aagctatcgg atccaaacct atgctGAATA TATGGGGAGA AAACAGGAAG 1621 AGAAGCAGAT CAAAAGAAAG TGGACTGAAG ATAGCTGGAA GGAGGCTGAC AGCAAACGGT 1681 TAAACCCCCA GTGCATACCC TACACCCTGC AGAATCACTA TTACCGCAAC AATAGTTTTA 1741 CAGCTCCCAG CACAGTAGGA AAGAGAAATT TGAGTCCTAG TCAAGAGGAA GCTGGCCTGG 1801 AGGACGGAGT GTCAGGGGAG ATTTCCGACA CTGAGCTTGA GCAGACCGAT TCCTGTGCAG 1861 AGCCCCTCTC AGAGGGAAGG AAAAAGGCCA AGAAATTAAA AAGAATGAAG AAGGAGCTTT 1921 CTCCAGCAGG AAGCATCTCG AAGAACAGCC CTGCCACACT CTTTGAAGTT CCTGACACAT 1981 GGTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2041 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2101 GGCTTTATAT ATCTTGTGGA AAGGACGAAA AGAAAACACC ACATCATTTC TCACGCGTTA 2161 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt