Transcript: Human XM_011525481.2

PREDICTED: Homo sapiens post-GPI attachment to proteins phospholipase 3 (PGAP3), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PGAP3 (93210)
Length:
2829
CDS:
531..1148

Additional Resources:

NCBI RefSeq record:
XM_011525481.2
NBCI Gene record:
PGAP3 (93210)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011525481.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000167276 CGACTGTAAGTATGAGTGTAT pLKO.1 241 5UTR 100% 4.950 3.960 N PGAP3 n/a
2 TRCN0000433431 TGGAACTGAGTGTGCTCTTAG pLKO_005 1403 3UTR 100% 10.800 7.560 N PGAP3 n/a
3 TRCN0000417559 AGCCTCATCCGCTTCGACTAT pLKO_005 828 CDS 100% 4.950 3.465 N PGAP3 n/a
4 TRCN0000172303 CCACTGTCATCCTACACTCAA pLKO.1 703 CDS 100% 4.950 3.465 N PGAP3 n/a
5 TRCN0000168629 GAAGGAATCAGAGGACAAGTT pLKO.1 1115 CDS 100% 4.950 3.465 N PGAP3 n/a
6 TRCN0000413925 TTGACTTCCCACCGCTCTTCT pLKO_005 1000 CDS 100% 4.950 3.465 N PGAP3 n/a
7 TRCN0000167247 CAACTTCTTGAACTTGGACAT pLKO.1 1241 3UTR 100% 4.050 2.835 N PGAP3 n/a
8 TRCN0000421008 GTCCCTCAATGCATGGTTCTG pLKO_005 620 CDS 100% 4.050 2.835 N PGAP3 n/a
9 TRCN0000172278 CCAGCCAATCTACATGAGTCT pLKO.1 199 5UTR 100% 2.640 1.848 N PGAP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011525481.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04598 pDONR223 100% 61.8% 57.5% None (many diffs) n/a
2 ccsbBroad304_04598 pLX_304 0% 61.8% 57.5% V5 (many diffs) n/a
3 TRCN0000465222 ACGCATCCCATACATCCGCCCGGC pLX_317 22.3% 61.8% 57.5% V5 (many diffs) n/a
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