Transcript: Human XM_011526231.2

PREDICTED: Homo sapiens zinc finger protein 397 (ZNF397), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF397 (84307)
Length:
10265
CDS:
102..1706

Additional Resources:

NCBI RefSeq record:
XM_011526231.2
NBCI Gene record:
ZNF397 (84307)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011526231.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232318 GCCATGGGACCACGCATAAAT pLKO_005 10112 3UTR 100% 15.000 21.000 N ZNF397 n/a
2 TRCN0000020174 CCGGAACAAGAACTAATACTA pLKO.1 150 CDS 100% 5.625 7.875 N ZNF397 n/a
3 TRCN0000020176 GAACCTTCATTTCGAGGAATT pLKO.1 726 CDS 100% 0.000 0.000 N ZNF397 n/a
4 TRCN0000232317 TGAAGAACACATCGTCAATAA pLKO_005 9807 3UTR 100% 13.200 10.560 N ZNF397 n/a
5 TRCN0000232316 CAGTGCCATGGAAGGATTTAA pLKO_005 541 CDS 100% 15.000 10.500 N ZNF397 n/a
6 TRCN0000232314 CCTCCGGAACAAGAACTAATA pLKO_005 147 CDS 100% 13.200 9.240 N ZNF397 n/a
7 TRCN0000232315 GCAGAATGGGAGTACTCAATC pLKO_005 212 CDS 100% 10.800 7.560 N ZNF397 n/a
8 TRCN0000020177 GAGGATTTAGAGAGGGAGTTT pLKO.1 477 CDS 100% 4.950 3.465 N ZNF397 n/a
9 TRCN0000020175 GCCCTTAAAGACACAGCTGAA pLKO.1 605 CDS 100% 4.050 2.835 N ZNF397 n/a
10 TRCN0000020178 GAACAGTGAAACAGCAACAAA pLKO.1 665 CDS 100% 5.625 3.375 N ZNF397 n/a
11 TRCN0000239754 GAGAAACCTTATGAGTGTAAT pLKO_005 1281 CDS 100% 13.200 6.600 Y Gm11677 n/a
12 TRCN0000139739 CCTTCTGAGTAGCTGGGATTA pLKO.1 3186 3UTR 100% 10.800 5.400 Y INAVA n/a
13 TRCN0000148104 GAGTGTAATGAATGTGGGAAA pLKO.1 1293 CDS 100% 4.050 2.025 Y ZNF658B n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4786 3UTR 100% 13.200 6.600 Y LIAS n/a
15 TRCN0000243782 TGGAGAGAAACCCTATGAATA pLKO_005 1445 CDS 100% 13.200 6.600 Y Zfp977 n/a
16 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3323 3UTR 100% 5.625 2.813 Y KLHL30 n/a
17 TRCN0000152004 CTGGTGAGAAACCTTATGAAT pLKO.1 1276 CDS 100% 5.625 2.813 Y ZNF829 n/a
18 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3323 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011526231.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04370 pDONR223 100% 47.3% 40.5% None (many diffs) n/a
2 ccsbBroad304_04370 pLX_304 0% 47.3% 40.5% V5 (many diffs) n/a
3 TRCN0000471015 TCATCTCTCTTTCTATCCGTAGTC pLX_317 44.8% 47.3% 40.5% V5 (many diffs) n/a
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