Transcript: Human XM_011527437.1

PREDICTED: Homo sapiens NSF attachment protein alpha (NAPA), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NAPA (8775)
Length:
1610
CDS:
384..959

Additional Resources:

NCBI RefSeq record:
XM_011527437.1
NBCI Gene record:
NAPA (8775)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011527437.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000380024 CAAGAGGCCATTAACTGTTTG pLKO_005 363 5UTR 100% 10.800 15.120 N NAPA n/a
2 TRCN0000381191 ACATGGGCCGATTCACGATTG pLKO_005 409 CDS 100% 6.000 8.400 N NAPA n/a
3 TRCN0000029170 CAGCGCCAAAGACTACTTCTT pLKO.1 671 CDS 100% 4.950 6.930 N NAPA n/a
4 TRCN0000293265 CAGCGCCAAAGACTACTTCTT pLKO_005 671 CDS 100% 4.950 6.930 N NAPA n/a
5 TRCN0000382195 TGAGTCATGAGGCCGGTTTCT pLKO_005 1310 3UTR 100% 4.950 6.930 N NAPA n/a
6 TRCN0000380266 TGCGAGCAATCGAGATCTACA pLKO_005 385 CDS 100% 4.950 6.930 N NAPA n/a
7 TRCN0000293268 TCTTTGATACAGACCTATTTG pLKO_005 1232 3UTR 100% 13.200 9.240 N NAPA n/a
8 TRCN0000029171 GCTGGCAACGCATTCAAGAAA pLKO.1 333 5UTR 100% 5.625 3.938 N NAPA n/a
9 TRCN0000382210 CACCCTCTGTTCTCGCTACAT pLKO_005 1142 3UTR 100% 4.950 3.465 N NAPA n/a
10 TRCN0000029169 CCGGGAATGCAAGTTGATGAA pLKO.1 785 CDS 100% 4.950 3.465 N NAPA n/a
11 TRCN0000293266 CCGGGAATGCAAGTTGATGAA pLKO_005 785 CDS 100% 4.950 3.465 N NAPA n/a
12 TRCN0000111543 CGCCAAAGACTACTTCTTCAA pLKO.1 674 CDS 100% 4.950 3.465 N Napa n/a
13 TRCN0000298396 CGCCAAAGACTACTTCTTCAA pLKO_005 674 CDS 100% 4.950 3.465 N Napa n/a
14 TRCN0000029173 GTATCAGAAGGCCATTGACAT pLKO.1 602 CDS 100% 4.950 3.465 N NAPA n/a
15 TRCN0000293264 GTATCAGAAGGCCATTGACAT pLKO_005 602 CDS 100% 4.950 3.465 N NAPA n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011527437.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02014 pDONR223 100% 64.7% 64.7% None 0_1ins312 n/a
2 ccsbBroad304_02014 pLX_304 0% 64.7% 64.7% V5 0_1ins312 n/a
3 TRCN0000472853 CTGTAGACAATTTAGACTTCCTCG pLX_317 46.2% 64.7% 64.7% V5 0_1ins312 n/a
Download CSV