Transcript: Human XM_011528933.2

PREDICTED: Homo sapiens zinc finger protein 512B (ZNF512B), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF512B (57473)
Length:
1691
CDS:
203..1597

Additional Resources:

NCBI RefSeq record:
XM_011528933.2
NBCI Gene record:
ZNF512B (57473)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011528933.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232816 CTACGGGCTCAAGTACCATTA pLKO_005 601 CDS 100% 10.800 15.120 N ZNF512B n/a
2 TRCN0000232817 TGAGCAAACCTGTCACTATTG pLKO_005 825 CDS 100% 10.800 15.120 N ZNF512B n/a
3 TRCN0000128121 CAAACCCATCACAGTCACCAA pLKO.1 1009 CDS 100% 2.640 3.696 N ZNF512B n/a
4 TRCN0000128650 CAAACCCATTACGGTAACAAA pLKO.1 1063 CDS 100% 5.625 3.938 N ZNF512B n/a
5 TRCN0000232818 TACCTGTCACCAAACCCATTA pLKO_005 1053 CDS 100% 0.000 0.000 N ZNF512B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011528933.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08732 pDONR223 100% 49.4% 46.5% None (many diffs) n/a
2 ccsbBroad304_08732 pLX_304 0% 49.4% 46.5% V5 (many diffs) n/a
3 TRCN0000470092 GCTGGTGTTTCCAAGGCATACGTT pLX_317 18.5% 49.4% 46.5% V5 (many diffs) n/a
4 ccsbBroadEn_08733 pDONR223 100% 49.3% 46.5% None (many diffs) n/a
5 ccsbBroad304_08733 pLX_304 0% 49.3% 46.5% V5 (many diffs) n/a
6 TRCN0000475732 ATACGGCGTCCGCCCCGCTACCTT pLX_317 12.9% 49.3% 46.5% V5 (many diffs) n/a
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