Construct: ORF TRCN0000470092
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008845.1_s317c1
- Derived from:
- ccsbBroadEn_08732
- DNA Barcode:
- GCTGGTGTTTCCAAGGCATACGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF512B (57473)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470092
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57473 | ZNF512B | zinc finger protein 512B | NM_020713.3 | 99.9% | 100% | 1314A>C |
| 2 | human | 57473 | ZNF512B | zinc finger protein 512B | XM_024451953.1 | 98.4% | 98.4% | 1_42del;1356A>C |
| 3 | human | 57473 | ZNF512B | zinc finger protein 512B | XM_011528930.2 | 94.7% | 94.8% | 1_42del;1356A>C;2368_2369ins99 |
| 4 | human | 57473 | ZNF512B | zinc finger protein 512B | XM_024451951.1 | 83.3% | 83.3% | 1314A>C;2327_2860del |
| 5 | human | 57473 | ZNF512B | zinc finger protein 512B | XM_024451952.1 | 83.3% | 83.3% | 1314A>C;2327_2860del |
| 6 | human | 57473 | ZNF512B | zinc finger protein 512B | XM_024451950.1 | 82.2% | 82.2% | 1_42del;1356A>C;2369_2902del |
| 7 | human | 57473 | ZNF512B | zinc finger protein 512B | XM_024451954.1 | 52.3% | 52% | (many diffs) |
| 8 | human | 57473 | ZNF512B | zinc finger protein 512B | XM_024451955.1 | 52.1% | 51.7% | (many diffs) |
| 9 | human | 57473 | ZNF512B | zinc finger protein 512B | XM_011528933.2 | 49.4% | 46.5% | (many diffs) |
| 10 | mouse | 269401 | Zfp512b | zinc finger protein 512B | NM_001164597.1 | 83.2% | 85.8% | (many diffs) |
| 11 | mouse | 269401 | Zfp512b | zinc finger protein 512B | XM_006500647.3 | 83.2% | 85.8% | (many diffs) |
| 12 | mouse | 269401 | Zfp512b | zinc finger protein 512B | XM_006500650.3 | 49.8% | 50.3% | (many diffs) |
| 13 | mouse | 269401 | Zfp512b | zinc finger protein 512B | XM_011239943.2 | 49.7% | 50.1% | (many diffs) |
| 14 | mouse | 269401 | Zfp512b | zinc finger protein 512B | XR_375397.3 | 46% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2745
- ORF length:
- 2676
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacggatcct ttctgcgttg gaggccgtcg gctcccgggg tccagcaaga 121 gtggtcccgg gaaggatggc agccgaaagg aggtccgact tccaatgctg catgacccac 181 cgaagatggg gatgccggtg gtccgtggtg gacagacagt gcccggccag gcccctctct 241 gctttgaccc gggaagtcca gccagtgaca agacagaagg gaagaaaaag gggcggccaa 301 aagccgagaa ccaggccctc cgagacattc ctctctccct gatgaacgac tggaaggatg 361 agttcaaggc acactcgagg gtgaagtgtc caaactcagg gtgctggctg gagttcccca 421 gcatctacgg gctcaagtac cattaccagc ggtgccaagg gggtgccatc tcagatcgcc 481 tggccttccc ctgccccttc tgcgaggccg cattcacctc taagacccag ctggagaaac 541 accggatctg gaaccacatg gaccgacccc tgcctgcctc caagcctggg cccatcagca 601 ggccggtcac catcagccgg cctgttgggg tcagcaagcc catcggagtg agcaaacctg 661 tcactattgg caaacctgtg ggtgtcagca aacccattgg catcagcaag ccagtctcgg 721 tcggcagacc catgccagtc accaaggcca tcccggtcac taggcccgtg ccagtcacca 781 aacctgtcac agtcagcagg cccatgcccg tcaccaaggc catgccggtc accaaaccca 841 tcacagtcac caagtctgtg ccggtcacca aacccgtacc tgtcaccaaa cccattacgg 901 taacaaagct tgtgacagtt acgaaacccg tgccggtcac caagccagtg acagtcagca 961 ggcccattgt ggtcagcaag ccggtgacag tcagcaggcc cattgctatc agcagacaca 1021 caccgccctg caaaatggtg ctgctgacca ggtcggagaa caaagcacct cgtgccacag 1081 ggaggaacag tggtaagaaa agggctgcgg acagcctgga cacctgccca attccaccca 1141 agcaggccag gccagagaat ggggagtacg gcccctcctc catgggccag agctcggcct 1201 tccagctgag tgcagacacc agcagtggct ccttgtcgcc aggcagcagg ccgtcagggg 1261 gcatggaggc actgaaggct gcaggccctg cgtccccgcc tgaggaggac ccggagcgca 1321 caaagcacag aaggaaacag aaaacaccca aaaagtttac aggggagcag ccatccatct 1381 ccgggacctt tgggctcaaa ggcctggtca aagctgagga caaggcccga gttcaccgct 1441 ccaagaagca ggaggggcca ggccctgagg acgcccggaa gaaggtgcca gctgccccca 1501 tcactgtcag caaggaggca ccggcccctg tggcccaccc agctccaggt ggccctgaag 1561 agcagtggca gagggccatc catgagcgcg gggaagccgt ctgccccacc tgcaacgtgg 1621 tcacccggaa gactctcgtg gggcttaaga agcacatgga ggtgtgtcag aagcttcagg 1681 atgcactcaa gtgccagcac tgccggaagc agttcaagtc caaagccggc ctcaactacc 1741 acactatggc cgagcacagt gccaagccct ctgacgccga ggcctccgaa gggggcgagc 1801 aggaggagcg cgagaggctg cgcaaggtgc tgaagcagat gggacggctg cgctgccccc 1861 aggagggttg cggggctgcc ttctccagcc tcatgggcta ccagtaccac cagcggcgct 1921 gcgggaagcc gccctgcgag gtggacagcc cctccttccc ctgcacccac tgtggcaaga 1981 cgtaccgatc caaggctggc cacgactacc acgtgcgctc ggagcacacg gccccccccc 2041 ctgaggagcc cacagacaag tcccctgagg ctgaggaccc gctgggtgtg gagcggaccc 2101 caagcgggcg tgtccgccgc acgtcggccc aggtggcggt gttccacctg caggagatag 2161 cggaggacga gctggcccgc gactggacca agcggcgcat gaaggatgac cttgtgcccg 2221 agaccgcacg gctcaactac actcgaccag ggcTCCCCAC GCTGAACCCC CAGCTGCTAG 2281 AGGCATGGAA GAATGAAGTG AAGGAGAAAG GCCACGTCAA CTGTCCCAAC GACTGCTGTG 2341 AAGCCATCTA CTCCAGCGTG TCCGGACTCA AGGCTCATCT CGCCAGCTGC AGTAAGGGGG 2401 CCCACCTGGC AGGGAAGTAC CGCTGTCTGC TGTGTCCGAA GGAGTTCAGT TCTGAGAGTG 2461 GCGTCAAATA CCACATCCTG AAGACCCACG CAGAGAACTG GTTCCGAACA TCAGCAGACC 2521 CACCTCCCAA ACACAGGAGC CAGGACTCAT TGGTGCCCAA GAAGGAAAAG AAGAAAAATC 2581 TGGCAGGTGG AAAGAAGCGG GGCCGAAAGC CCAAGGAGCG GACCCCAGAG GAGCCTGTGG 2641 CCAAGCTGCC CCCGCGCCGG GACGACTGGC CTCCAGGATG CAGAGACAAG GGGGCCCGGG 2701 GCTCCACCGG CCGGAAGGTG GGAGTCAGCA AGGCGCCTGA AAAGTTGCCA ACTTTCTTGT 2761 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2821 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2881 GAAAGGACGA GCTGGTGTTT CCAAGGCATA CGTTACGCGT TAAGTCgaca atcaacctct 2941 ggattacaaa atttgtgaaa gatt