Transcript: Human XM_011529309.1

PREDICTED: Homo sapiens Ral GTPase activating protein catalytic alpha subunit 2 (RALGAPA2), transcript variant X6, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RALGAPA2 (57186)
Length:
6422
CDS:
159..5819

Additional Resources:

NCBI RefSeq record:
XM_011529309.1
NBCI Gene record:
RALGAPA2 (57186)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011529309.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000264207 GACCGAACCGATGCGATTTAG pLKO_005 2273 CDS 100% 13.200 18.480 N RALGAPA2 n/a
2 TRCN0000264209 TAAACGAGTGGGCCAACATTA pLKO_005 2068 CDS 100% 13.200 18.480 N RALGAPA2 n/a
3 TRCN0000264210 TACGGGATAATCTAGCAATAA pLKO_005 3202 CDS 100% 13.200 18.480 N RALGAPA2 n/a
4 TRCN0000282944 ACTTGTCATCCACGGATTATG pLKO_005 4270 CDS 100% 13.200 9.240 N RALGAPA2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011529309.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14227 pDONR223 100% 11.8% 11.7% None (many diffs) n/a
2 ccsbBroad304_14227 pLX_304 0% 11.8% 11.7% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000467052 AACGGTAAACGTGCGTAGCCTCTG pLX_317 64.2% 11.8% 11.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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