Construct: ORF TRCN0000467052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016368.1_s317c1
- Derived from:
- ccsbBroadEn_14227
- DNA Barcode:
- AACGGTAAACGTGCGTAGCCTCTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RALGAPA2 (57186)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_017027976.1 | 17.9% | 18% | 1_3180del;3893_3963del |
| 2 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_011529311.2 | 17.6% | 17.7% | 1_3099del;3812_4032del |
| 3 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_017027975.1 | 17.3% | 17.3% | 1_3180del;3893_4113del |
| 4 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_017027973.1 | 14.1% | 14.2% | 1_4089del;4802_5022del |
| 5 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_017027974.1 | 14.1% | 14.2% | 1_4089del;4802_5022del |
| 6 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_011529310.2 | 13.1% | 13.1% | 1_4491del;5204_5424del |
| 7 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | NM_020343.4 | 12.6% | 12.7% | 1_4836del;5549_5619del |
| 8 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_006723599.1 | 12.3% | 12.3% | 1_4977del;5690_5760del |
| 9 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_005260768.2 | 12.3% | 12.3% | 1_4836del;5549_5769del |
| 10 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_006723598.1 | 12% | 12% | 1_4977del;5690_5910del |
| 11 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_011529309.1 | 11.8% | 11.7% | (many diffs) |
| 12 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XM_017027972.1 | 9.7% | 9.6% | (many diffs) |
| 13 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XR_937111.2 | 7.2% | 1_5135del;5848_9866del | |
| 14 | human | 57186 | RALGAPA2 | Ral GTPase activating prote... | XR_001754352.1 | 6.3% | 1_5135del;5848_11290del | |
| 15 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | NM_001033348.3 | 11.3% | 12.3% | (many diffs) |
| 16 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | XM_006499519.3 | 11% | 12% | (many diffs) |
| 17 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | XM_006499517.3 | 11% | 11.9% | (many diffs) |
| 18 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | XR_866276.2 | 6.5% | (many diffs) | |
| 19 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | XR_866277.2 | 6.5% | (many diffs) | |
| 20 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | XR_866275.2 | 5.5% | (many diffs) | |
| 21 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | XR_374470.3 | 5.3% | (many diffs) | |
| 22 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | XR_374469.3 | 5.1% | (many diffs) | |
| 23 | mouse | 241694 | Ralgapa2 | Ral GTPase activating prote... | XR_374468.3 | 5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttcttgggac agaaggaaga attttcatct attgaagaaa aattcaaaat 121 tattgagaga gctgaaaaat ttggactccc gccagtgccg tgagacacac aaaatcgcag 181 tgttttacat tgctgaaggt caagaagaca agtgttcaat cctctctaat gaaagaggaa 241 gccaagcata tgaagacttt gttgctggac ttggatggga ggtggatctc tccacccact 301 gtgggttcat gggtggccTT CAGCGCAATG GCAGCACCGG GCAGACGGCC CCTTACTATG 361 CTACCTCAAC TGTGGAAGTG ATTTTCCATG TTTCCACTCG AATGCCGTCA GACTCAGATG 421 ATTCCCTCAC CAAAAAGCTT CGTCACTTGG GGAATGACGA GGTCCATATC GTCTGGTCTG 481 AACACTCCAG AGACTACCGC AGGGGTATTA TCCCAACTGC CTTTGGAGAT GTTTCAATCA 541 TTATTTACCC AATGAAGAAT CACATGTTCT TCATCGCGAT AACGAAGAAA CCTGAGGTTC 601 CATTTTTTGG GCCTCTGTTT GATGGAGCCA TAGTGAGTGG GAAGCTGCTG CCAAGCCTTG 661 TATGTGCCAC GTGCATCAAC GCCAGCAGGG CTGTGAAGTG CCTCATCCCA CTCTACCAGA 721 GCTTCTATGA AGAGCGAGCT CTGTATCTCG AAGCAATAAT TCAGAACCAC CGCGAAGTAC 781 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 841 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 901 TATATATCTT GTGGAAAGGA CGAAACGGTA AACGTGCGTA GCCTCTGACG CGTTAAGTCg 961 acaatcaacc tctggattac aaaatttgtg aaagatt