Transcript: Human XM_011529387.2

PREDICTED: Homo sapiens minichromosome maintenance 8 homologous recombination repair factor (MCM8), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MCM8 (84515)
Length:
2187
CDS:
341..2086

Additional Resources:

NCBI RefSeq record:
XM_011529387.2
NBCI Gene record:
MCM8 (84515)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011529387.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000285482 GGATCGATTCATACCATATAA pLKO_005 547 CDS 100% 15.000 21.000 N MCM8 n/a
2 TRCN0000020277 CGGGAGACACAGTGACTATTA pLKO.1 1314 CDS 100% 13.200 18.480 N MCM8 n/a
3 TRCN0000276010 CGGGAGACACAGTGACTATTA pLKO_005 1314 CDS 100% 13.200 18.480 N MCM8 n/a
4 TRCN0000020274 GCAAAGTATTAGTCTTGCTAA pLKO.1 1948 CDS 100% 4.950 6.930 N MCM8 n/a
5 TRCN0000020278 GCTCGTATGAATAGTCAAGAT pLKO.1 2166 3UTR 100% 4.950 6.930 N MCM8 n/a
6 TRCN0000276011 GCTCGTATGAATAGTCAAGAT pLKO_005 2166 3UTR 100% 4.950 6.930 N MCM8 n/a
7 TRCN0000020276 CCTTTGATTGAGAAGATTCAA pLKO.1 611 CDS 100% 5.625 3.938 N MCM8 n/a
8 TRCN0000276065 CCTTTGATTGAGAAGATTCAA pLKO_005 611 CDS 100% 5.625 3.938 N MCM8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011529387.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09195 pDONR223 100% 69.1% 68.8% None 1372C>T;1733_1734ins50;1743_1744ins727 n/a
2 ccsbBroad304_09195 pLX_304 0% 69.1% 68.8% V5 1372C>T;1733_1734ins50;1743_1744ins727 n/a
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