Construct: ORF ccsbBroad304_09195

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017091.1_s304c1, BRDN0000404794
Derived from:
ccsbBroadEn_09195
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MCM8 (84515)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_09195
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84515 MCM8 minichromosome maintenance ... NM_001281520.1 99.9% 100% 1372C>T
2 human 84515 MCM8 minichromosome maintenance ... NM_032485.6 99.9% 100% 1372C>T
3 human 84515 MCM8 minichromosome maintenance ... NM_182802.3 98% 98% 1023_1024ins48;1324C>T
4 human 84515 MCM8 minichromosome maintenance ... NM_001281521.1 95% 94.6% (many diffs)
5 human 84515 MCM8 minichromosome maintenance ... XM_017028105.1 95% 94.6% (many diffs)
6 human 84515 MCM8 minichromosome maintenance ... NM_001281522.1 94.4% 94.4% 1254_1255ins141
7 human 84515 MCM8 minichromosome maintenance ... XM_017028106.1 92.3% 92.3% 0_1ins192;1180C>T
8 human 84515 MCM8 minichromosome maintenance ... XM_011529387.2 69.1% 68.8% 1372C>T;1733_1734ins50;1743_1744ins727
9 human 84515 MCM8 minichromosome maintenance ... XM_017028107.1 66.2% 65.4% 0_1ins763;26_27ins86;523C>T
10 human 84515 MCM8 minichromosome maintenance ... XR_001754422.1 26.5% (many diffs)
11 human 84515 MCM8 minichromosome maintenance ... XR_001754423.1 24.9% 1_340del;1563_1564ins172;2689_9241del
12 mouse 66634 Mcm8 minichromosome maintenance ... NM_001291054.1 86.3% 89.1% (many diffs)
13 mouse 66634 Mcm8 minichromosome maintenance ... NM_025676.4 83.5% 86.5% (many diffs)
14 mouse 66634 Mcm8 minichromosome maintenance ... XM_006500021.3 59.4% 60.7% (many diffs)
15 mouse 66634 Mcm8 minichromosome maintenance ... XR_374505.2 43.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2586
ORF length:
2520
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa tggagagtat agaggcagag gatttggacg aggaagattt caaagctgga
121aaaggggaag aggtggtggg aacttctcag gaaaatggag agaaagagaa cacagacctg
181atctgagtaa aaccacagga aaacgtactt ctgaacaaac cccacagttt ttgctttcaa
241caaagacccc acagtcaatg cagtcaacat tggatcgatt cataccatat aaaggctgga
301agctttattt ctctgaagtt tacagcgata gctctccttt gattgagaag attcaagcat
361ttgaaaaatt tttcacaagg catattgatt tgtatgacaa ggatgaaata gaaagaaagg
421gaagtatttt ggtagatttt aaagaactga cagaaggtgg tgaagtaact aacttgatac
481cagatatagc aactgaacta agagatgcac ctgagaaaac cttggcttgc atgggtttgg
541caatacatca ggtgttaact aaggaccttg aaaggcatgc agctgagtta caagcccagg
601aaggattgtc taatgatgga gaaacaatgg taaatgtgcc acatattcat gcaagggtgt
661acaactatga gcctttgaca cagctcaaga atgtcagagc aaattactat ggaaaataca
721ttgctctaag agggacagtg gttcgtgtca gtaatataaa gcctctttgc accaagatgg
781cttttctttg tgctgcatgt ggagaaattc agagctttcc tcttccagat ggaaaataca
841gtcttcccac aaagtgtcct gtgcctgtgt gtcgaggcag gtcatttact gctctccgca
901gctctcctct cacagttacg atggactggc agtcaatcaa aatccaggaa ttgatgtctg
961atgatcagag agaagcaggt cggattccac gaacaataga atgtgagctt gttcatgatc
1021ttgtggatag ctgtgtcccg ggagacacag tgactattac tggaattgtc aaagtctcaa
1081atgcggaaga aggttctcga aataagaatg acaagtgtat gttccttttg tatattgaag
1141caaattctat tagtaatagc aaaggacaga aaacaaagag ttctgaggat gggtgtaagc
1201atggaatgtt gatggagttc tcacttaaag acctttatgc catccaagag attcaagctg
1261aagaaaacct gtttaaactc attgtcaact cgctttgccc tgtcattttt ggtcatgaac
1321ttgttaaagc aggtttggca ttagcactct ttggaggaag ccagaaatac gcagatgaca
1381aaaacagaat tccaattcgg ggagaccccc acatccttgt tgttggagat ccaggcttag
1441gaaaaagtca aatgctacag gcagcgtgca atgttgcccc acgtggcgtg tatgtttgtg
1501gtaacaccac gaccacctct ggtctgacgg taactctttc aaaagatagt tcctctggag
1561attttgcttt ggaagctggt gccctggtac ttggtgatca aggtatttgt ggaatcgatg
1621aatttgataa gatggggaat caacatcaag ccttgttgga agccatggag cagcaaagta
1681ttagtcttgc taaggctggt gtggtttgta gccttcctgc aagaacttcc attattgctg
1741ctgcaaatcc agttggagga cattacaata aagccaaaac agtttctgag aatttaaaaa
1801tggggagtgc actactatcc agatttgatt tggtctttat cctgttagat actccaaatg
1861agcatcatga tcacttactc tctgaacatg tgattgcaat aagagctgga aagcagagaa
1921ccattagcag tgccacagta gctcgtatga atagtcaaga ttcaaatact tccgtacttg
1981aagtagtttc tgagaagcca ttatcagaaa gactaaaggt ggttcctgga gaaacaatag
2041atcccattcc ccaccagcta ttgagaaagt acattggcta tgctcggcag tatgtgtacc
2101caaggctatc cacagaagct gctcgagttc ttcaagattt ttaccttgag ctccggaaac
2161agagccagag gttaaatagc tcaccaatca ctaccaggca gctggaatct ttgattcgtc
2221tgacagaggc acgagcaagg ttggaattga gagaggaagc aaccaaagaa gacgctgagg
2281atatagtgga aattatgaaa tatagcatgc taggaactta ctctgatgaa tttgggaacc
2341tagattttga gcgatcccag catggttctg gaatgagcaa caggtcaaca gcgaaaagat
2401ttatttctgc tctcaacaac gttgctgaaa gaacttataa taatatattt caatttcatc
2461aacttcggca gattgccaaa gaactaaaca ttcaggttgc tgattttgaa aattttattg
2521gatcactaaa tgaccagggt tacctcttga aaaaaggccc aaaagtttac cagcttcaaa
2581ctatgtaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
2641gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2701acctctggat ta

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