Construct: ORF ccsbBroad304_09195
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017091.1_s304c1, BRDN0000404794
- Derived from:
- ccsbBroadEn_09195
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCM8 (84515)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_09195
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84515 | MCM8 | minichromosome maintenance ... | NM_001281520.1 | 99.9% | 100% | 1372C>T |
| 2 | human | 84515 | MCM8 | minichromosome maintenance ... | NM_032485.6 | 99.9% | 100% | 1372C>T |
| 3 | human | 84515 | MCM8 | minichromosome maintenance ... | NM_182802.3 | 98% | 98% | 1023_1024ins48;1324C>T |
| 4 | human | 84515 | MCM8 | minichromosome maintenance ... | NM_001281521.1 | 95% | 94.6% | (many diffs) |
| 5 | human | 84515 | MCM8 | minichromosome maintenance ... | XM_017028105.1 | 95% | 94.6% | (many diffs) |
| 6 | human | 84515 | MCM8 | minichromosome maintenance ... | NM_001281522.1 | 94.4% | 94.4% | 1254_1255ins141 |
| 7 | human | 84515 | MCM8 | minichromosome maintenance ... | XM_017028106.1 | 92.3% | 92.3% | 0_1ins192;1180C>T |
| 8 | human | 84515 | MCM8 | minichromosome maintenance ... | XM_011529387.2 | 69.1% | 68.8% | 1372C>T;1733_1734ins50;1743_1744ins727 |
| 9 | human | 84515 | MCM8 | minichromosome maintenance ... | XM_017028107.1 | 66.2% | 65.4% | 0_1ins763;26_27ins86;523C>T |
| 10 | human | 84515 | MCM8 | minichromosome maintenance ... | XR_001754422.1 | 26.5% | (many diffs) | |
| 11 | human | 84515 | MCM8 | minichromosome maintenance ... | XR_001754423.1 | 24.9% | 1_340del;1563_1564ins172;2689_9241del | |
| 12 | mouse | 66634 | Mcm8 | minichromosome maintenance ... | NM_001291054.1 | 86.3% | 89.1% | (many diffs) |
| 13 | mouse | 66634 | Mcm8 | minichromosome maintenance ... | NM_025676.4 | 83.5% | 86.5% | (many diffs) |
| 14 | mouse | 66634 | Mcm8 | minichromosome maintenance ... | XM_006500021.3 | 59.4% | 60.7% | (many diffs) |
| 15 | mouse | 66634 | Mcm8 | minichromosome maintenance ... | XR_374505.2 | 43.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2586
- ORF length:
- 2520
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tggagagtat agaggcagag gatttggacg aggaagattt caaagctgga 121 aaaggggaag aggtggtggg aacttctcag gaaaatggag agaaagagaa cacagacctg 181 atctgagtaa aaccacagga aaacgtactt ctgaacaaac cccacagttt ttgctttcaa 241 caaagacccc acagtcaatg cagtcaacat tggatcgatt cataccatat aaaggctgga 301 agctttattt ctctgaagtt tacagcgata gctctccttt gattgagaag attcaagcat 361 ttgaaaaatt tttcacaagg catattgatt tgtatgacaa ggatgaaata gaaagaaagg 421 gaagtatttt ggtagatttt aaagaactga cagaaggtgg tgaagtaact aacttgatac 481 cagatatagc aactgaacta agagatgcac ctgagaaaac cttggcttgc atgggtttgg 541 caatacatca ggtgttaact aaggaccttg aaaggcatgc agctgagtta caagcccagg 601 aaggattgtc taatgatgga gaaacaatgg taaatgtgcc acatattcat gcaagggtgt 661 acaactatga gcctttgaca cagctcaaga atgtcagagc aaattactat ggaaaataca 721 ttgctctaag agggacagtg gttcgtgtca gtaatataaa gcctctttgc accaagatgg 781 cttttctttg tgctgcatgt ggagaaattc agagctttcc tcttccagat ggaaaataca 841 gtcttcccac aaagtgtcct gtgcctgtgt gtcgaggcag gtcatttact gctctccgca 901 gctctcctct cacagttacg atggactggc agtcaatcaa aatccaggaa ttgatgtctg 961 atgatcagag agaagcaggt cggattccac gaacaataga atgtgagctt gttcatgatc 1021 ttgtggatag ctgtgtcccg ggagacacag tgactattac tggaattgtc aaagtctcaa 1081 atgcggaaga aggttctcga aataagaatg acaagtgtat gttccttttg tatattgaag 1141 caaattctat tagtaatagc aaaggacaga aaacaaagag ttctgaggat gggtgtaagc 1201 atggaatgtt gatggagttc tcacttaaag acctttatgc catccaagag attcaagctg 1261 aagaaaacct gtttaaactc attgtcaact cgctttgccc tgtcattttt ggtcatgaac 1321 ttgttaaagc aggtttggca ttagcactct ttggaggaag ccagaaatac gcagatgaca 1381 aaaacagaat tccaattcgg ggagaccccc acatccttgt tgttggagat ccaggcttag 1441 gaaaaagtca aatgctacag gcagcgtgca atgttgcccc acgtggcgtg tatgtttgtg 1501 gtaacaccac gaccacctct ggtctgacgg taactctttc aaaagatagt tcctctggag 1561 attttgcttt ggaagctggt gccctggtac ttggtgatca aggtatttgt ggaatcgatg 1621 aatttgataa gatggggaat caacatcaag ccttgttgga agccatggag cagcaaagta 1681 ttagtcttgc taaggctggt gtggtttgta gccttcctgc aagaacttcc attattgctg 1741 ctgcaaatcc agttggagga cattacaata aagccaaaac agtttctgag aatttaaaaa 1801 tggggagtgc actactatcc agatttgatt tggtctttat cctgttagat actccaaatg 1861 agcatcatga tcacttactc tctgaacatg tgattgcaat aagagctgga aagcagagaa 1921 ccattagcag tgccacagta gctcgtatga atagtcaaga ttcaaatact tccgtacttg 1981 aagtagtttc tgagaagcca ttatcagaaa gactaaaggt ggttcctgga gaaacaatag 2041 atcccattcc ccaccagcta ttgagaaagt acattggcta tgctcggcag tatgtgtacc 2101 caaggctatc cacagaagct gctcgagttc ttcaagattt ttaccttgag ctccggaaac 2161 agagccagag gttaaatagc tcaccaatca ctaccaggca gctggaatct ttgattcgtc 2221 tgacagaggc acgagcaagg ttggaattga gagaggaagc aaccaaagaa gacgctgagg 2281 atatagtgga aattatgaaa tatagcatgc taggaactta ctctgatgaa tttgggaacc 2341 tagattttga gcgatcccag catggttctg gaatgagcaa caggtcaaca gcgaaaagat 2401 ttatttctgc tctcaacaac gttgctgaaa gaacttataa taatatattt caatttcatc 2461 aacttcggca gattgccaaa gaactaaaca ttcaggttgc tgattttgaa aattttattg 2521 gatcactaaa tgaccagggt tacctcttga aaaaaggccc aaaagtttac cagcttcaaa 2581 ctatgtaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 2641 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 2701 acctctggat ta