Transcript: Human XM_011536663.2

PREDICTED: Homo sapiens testis specific serine kinase 4 (TSSK4), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TSSK4 (283629)
Length:
1175
CDS:
285..1073

Additional Resources:

NCBI RefSeq record:
XM_011536663.2
NBCI Gene record:
TSSK4 (283629)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001148876 TAAGAAGGGCTACAACCCAC pXPR_003 AGG 339 43% 3 0.9388 TSSK4 TSSK4 77044
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011536663.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000010698 TGCTGCTGGTAGGGACTTAAA pLKO.1 515 CDS 100% 13.200 18.480 N TSSK4 n/a
2 TRCN0000002414 CGTCATCCTTTACACTCTAGT pLKO.1 764 CDS 100% 4.950 6.930 N TSSK4 n/a
3 TRCN0000195194 CGAGTATACATCATTCTGGAA pLKO.1 345 CDS 100% 2.640 3.696 N TSSK4 n/a
4 TRCN0000002416 CAGCTCCACAACACCACTAAA pLKO.1 1023 CDS 100% 13.200 9.240 N TSSK4 n/a
5 TRCN0000002417 ACACCACTAAACAGCACCAAT pLKO.1 1033 CDS 100% 4.950 3.465 N TSSK4 n/a
6 TRCN0000199460 GCGGCACAAGTACCTCATCAA pLKO.1 296 CDS 100% 4.950 3.465 N TSSK4 n/a
7 TRCN0000199342 CCAGAGATCTTACGAGGCTTG pLKO.1 705 CDS 100% 2.250 1.575 N TSSK4 n/a
8 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 32 5UTR 100% 5.625 2.813 Y KLHL30 n/a
9 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 32 5UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011536663.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13502 pDONR223 100% 96.1% 96.1% None 212_241del n/a
2 ccsbBroad304_13502 pLX_304 0% 96.1% 96.1% V5 212_241del n/a
3 TRCN0000474270 ACCTGAGATACATACTTGTATTAA pLX_317 29.7% 96.1% 96.1% V5 212_241del n/a
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