Transcript: Human XM_011540975.3

PREDICTED: Homo sapiens EPH receptor A8 (EPHA8), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
EPHA8 (2046)
Length:
3965
CDS:
316..2118

Additional Resources:

NCBI RefSeq record:
XM_011540975.3
NBCI Gene record:
EPHA8 (2046)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001146550 CGTCAGGCAGATCCAGACAA pXPR_003 TGG 415 23% 5 0.4421 EPHA8 EPHA8 76527
2 BRDN0001146734 CCCAGAGCCCCAGTTCTATG pXPR_003 CGG 613 34% 7 -0.3027 EPHA8 EPHA8 76529
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011540975.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195445 CAGTGTGAATGGGACATCAGT pLKO.1 111 5UTR 100% 3.000 2.400 N EPHA8 n/a
2 TRCN0000197138 GCATGGACGTGTGCATGTTAT pLKO.1 2520 3UTR 100% 13.200 9.240 N EPHA8 n/a
3 TRCN0000196913 GAGCGTGTGCTTATCCGTTAA pLKO.1 2541 3UTR 100% 10.800 7.560 N EPHA8 n/a
4 TRCN0000230033 GAGCGTGTGCTTATCCGTTAA pLKO_005 2541 3UTR 100% 10.800 7.560 N EPHA8 n/a
5 TRCN0000230031 GCAGTGACATCACCTACAATG pLKO_005 170 5UTR 100% 10.800 7.560 N EPHA8 n/a
6 TRCN0000367486 AGCATGGACGTGTGCATGTTA pLKO_005 2519 3UTR 100% 5.625 3.938 N EPHA8 n/a
7 TRCN0000195591 CGCAGTGACATCACCTACAAT pLKO.1 169 5UTR 100% 5.625 3.938 N EPHA8 n/a
8 TRCN0000001345 CGAGCTGTTCTTCCTTCCTAT pLKO.1 3696 3UTR 100% 4.950 3.465 N EPHA8 n/a
9 TRCN0000001346 GACGAGGAGAAGATGCACTAT pLKO.1 832 CDS 100% 4.950 3.465 N EPHA8 n/a
10 TRCN0000196492 GAGTATGAGATCAAGTACTAC pLKO.1 514 CDS 100% 4.950 3.465 N EPHA8 n/a
11 TRCN0000230032 GAGTATGAGATCAAGTACTAC pLKO_005 514 CDS 100% 4.950 3.465 N EPHA8 n/a
12 TRCN0000356088 GCAATTCGACCATCCCAACAT pLKO_005 1167 CDS 100% 4.950 3.465 N EPHA8 n/a
13 TRCN0000356065 GGAGAAGATGCACTATCAGAA pLKO_005 837 CDS 100% 4.950 3.465 N EPHA8 n/a
14 TRCN0000377266 TTCTGGATCGAGGCCGTCAAT pLKO_005 328 CDS 100% 4.950 3.465 N EPHA8 n/a
15 TRCN0000001349 CAGATTGTCAGTGTCCTCGAT pLKO.1 1759 CDS 100% 2.640 1.848 N EPHA8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011540975.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.