Transcript: Human XM_011541608.1

PREDICTED: Homo sapiens spermatogenesis associated 6 (SPATA6), transcript variant X12, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SPATA6 (54558)
Length:
4823
CDS:
462..1604

Additional Resources:

NCBI RefSeq record:
XM_011541608.1
NBCI Gene record:
SPATA6 (54558)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011541608.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000061812 CCATTTGGATGACGGTGAATA pLKO.1 1451 CDS 100% 13.200 10.560 N SPATA6 n/a
2 TRCN0000248456 TCTGGACACCATGATTCAAAC pLKO_005 486 CDS 100% 10.800 7.560 N Spata6 n/a
3 TRCN0000248457 TGAGATCCATGACCGGGTAAA pLKO_005 1268 CDS 100% 10.800 7.560 N Spata6 n/a
4 TRCN0000061811 CCTCCATTTGTGATTAGACAT pLKO.1 897 CDS 100% 4.950 3.465 N SPATA6 n/a
5 TRCN0000061809 CGCATGTGTGAGCTATCTGAA pLKO.1 810 CDS 100% 4.950 3.465 N SPATA6 n/a
6 TRCN0000061808 GCCCTGTGTTAAATAGAGCTT pLKO.1 1198 CDS 100% 2.640 1.848 N SPATA6 n/a
7 TRCN0000061810 CGACTTCAGTGATTACTGAAT pLKO.1 571 CDS 100% 0.495 0.347 N SPATA6 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3155 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011541608.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12060 pDONR223 100% 74.5% 74.5% None 0_1ins324;823_870del n/a
2 ccsbBroad304_12060 pLX_304 0% 74.5% 74.5% V5 0_1ins324;823_870del n/a
3 TRCN0000465803 ATGTTGCTTGATGAAGCCAGGCTC pLX_317 23.8% 74.5% 74.5% V5 0_1ins324;823_870del n/a
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