Transcript: Human XM_011544194.3

PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MTR (4548)
Length:
11318
CDS:
1018..4983

Additional Resources:

NCBI RefSeq record:
XM_011544194.3
NBCI Gene record:
MTR (4548)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011544194.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000424139 GTTTCTACCTCAGGTTATAAA pLKO_005 3369 CDS 100% 15.000 21.000 N MTR n/a
2 TRCN0000413568 AGAGTATCCAAGACGACATTC pLKO_005 4298 CDS 100% 10.800 15.120 N MTR n/a
3 TRCN0000035382 CCCAAGATATTTAACGACAAA pLKO.1 4165 CDS 100% 4.950 6.930 N MTR n/a
4 TRCN0000035383 GCTGTAATGTTGCAGGATCAA pLKO.1 2315 CDS 100% 4.950 6.930 N MTR n/a
5 TRCN0000251688 CCAGTGAAGCCCACGTTTATT pLKO_005 4033 CDS 100% 15.000 10.500 N Mtr n/a
6 TRCN0000424357 ATATTAGACAGGACCATTATG pLKO_005 3929 CDS 100% 13.200 9.240 N MTR n/a
7 TRCN0000429125 CAGTGAAGCCCACGTTTATTG pLKO_005 4034 CDS 100% 13.200 9.240 N MTR n/a
8 TRCN0000035381 CCAGATCATATCAGGGAAATT pLKO.1 2167 CDS 100% 13.200 9.240 N MTR n/a
9 TRCN0000424187 TTCAGGGACGATCGTTGATAA pLKO_005 1857 CDS 100% 13.200 9.240 N MTR n/a
10 TRCN0000035380 CCAGAATAAGTGGAGGTCTTT pLKO.1 2897 CDS 100% 4.950 3.465 N MTR n/a
11 TRCN0000035379 GCTGCAATAATTTCCGAGTTA pLKO.1 3575 CDS 100% 4.950 3.465 N MTR n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011544194.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13904 pDONR223 100% 91.9% 30.1% None (many diffs) n/a
2 ccsbBroad304_13904 pLX_304 0% 91.9% 30.1% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000481034 TCTCGTACTTCTAGTCTTGCAGTC pLX_317 11.2% 91.9% 30.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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