Transcript: Human XM_017001329.2

PREDICTED: Homo sapiens 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MTR (4548)
Length:
11165
CDS:
1018..4830

Additional Resources:

NCBI RefSeq record:
XM_017001329.2
NBCI Gene record:
MTR (4548)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017001329.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413568 AGAGTATCCAAGACGACATTC pLKO_005 4145 CDS 100% 10.800 15.120 N MTR n/a
2 TRCN0000035382 CCCAAGATATTTAACGACAAA pLKO.1 4012 CDS 100% 4.950 6.930 N MTR n/a
3 TRCN0000035383 GCTGTAATGTTGCAGGATCAA pLKO.1 2315 CDS 100% 4.950 6.930 N MTR n/a
4 TRCN0000251688 CCAGTGAAGCCCACGTTTATT pLKO_005 3880 CDS 100% 15.000 10.500 N Mtr n/a
5 TRCN0000424357 ATATTAGACAGGACCATTATG pLKO_005 3776 CDS 100% 13.200 9.240 N MTR n/a
6 TRCN0000429125 CAGTGAAGCCCACGTTTATTG pLKO_005 3881 CDS 100% 13.200 9.240 N MTR n/a
7 TRCN0000035381 CCAGATCATATCAGGGAAATT pLKO.1 2167 CDS 100% 13.200 9.240 N MTR n/a
8 TRCN0000424187 TTCAGGGACGATCGTTGATAA pLKO_005 1857 CDS 100% 13.200 9.240 N MTR n/a
9 TRCN0000035380 CCAGAATAAGTGGAGGTCTTT pLKO.1 2897 CDS 100% 4.950 3.465 N MTR n/a
10 TRCN0000035379 GCTGCAATAATTTCCGAGTTA pLKO.1 3422 CDS 100% 4.950 3.465 N MTR n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017001329.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13904 pDONR223 100% 88.1% 31.2% None (many diffs) n/a
2 ccsbBroad304_13904 pLX_304 0% 88.1% 31.2% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000481034 TCTCGTACTTCTAGTCTTGCAGTC pLX_317 11.2% 88.1% 31.2% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV