Transcript: Human XM_017002582.2

PREDICTED: Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant X17, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CDC42BPA (8476)
Length:
6122
CDS:
969..5738

Additional Resources:

NCBI RefSeq record:
XM_017002582.2
NBCI Gene record:
CDC42BPA (8476)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145566 AAAACTGTAGGAACTAGTCC pXPR_003 AGG 1654 35% 13 0.622 CDC42BPA CDC42BPA 75746
2 BRDN0001147767 CAACATAATAATCCATAACC pXPR_003 AGG 458 10% 5 0.5663 CDC42BPA CDC42BPA 75748
3 BRDN0001146626 TTCTGAGGATCTATACCCAG pXPR_003 GGG 3180 67% 24 0.0576 CDC42BPA CDC42BPA 75747
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017002582.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195596 CCAGGCTAGTGAGCGATTAAA pLKO.1 2624 CDS 100% 15.000 21.000 N CDC42BPA n/a
2 TRCN0000195557 CCGATGCTCTGGATCAATTTG pLKO.1 3850 CDS 100% 13.200 18.480 N CDC42BPA n/a
3 TRCN0000194832 CGGAAAGATATACCCTGTATA pLKO.1 4395 CDS 100% 13.200 18.480 N CDC42BPA n/a
4 TRCN0000000662 GCAAGAGATAACCAAACTAAA pLKO.1 3017 CDS 100% 13.200 18.480 N CDC42BPA n/a
5 TRCN0000001333 GCAAGAGATAACCAAACTAAA pLKO.1 3017 CDS 100% 13.200 18.480 N CDC42BPA n/a
6 TRCN0000022979 CCAGGATGACAATAACTTATA pLKO.1 1400 CDS 100% 13.200 10.560 N LOC381309 n/a
7 TRCN0000196514 GTGGAATTGATTGGGATAATA pLKO.1 1999 CDS 100% 15.000 10.500 N CDC42BPA n/a
8 TRCN0000194982 CAGTTAGAAGAAGAGGTTAAA pLKO.1 3363 CDS 100% 13.200 9.240 N CDC42BPA n/a
9 TRCN0000196893 GTTGTTACAATGCACCATATC pLKO.1 5293 CDS 100% 10.800 7.560 N CDC42BPA n/a
10 TRCN0000000660 GCGATTACATAGAGAAGACTT pLKO.1 1178 CDS 100% 4.950 3.465 N CDC42BPA n/a
11 TRCN0000001331 GCGATTACATAGAGAAGACTT pLKO.1 1178 CDS 100% 4.950 3.465 N CDC42BPA n/a
12 TRCN0000000661 CGCTCAGTCTATGTTCCCAAA pLKO.1 4560 CDS 100% 4.050 2.835 N CDC42BPA n/a
13 TRCN0000001332 CGCTCAGTCTATGTTCCCAAA pLKO.1 4560 CDS 100% 4.050 2.835 N CDC42BPA n/a
14 TRCN0000199935 GCTCGCCATGTCCGAGATAAG pLKO.1 2757 CDS 100% 3.600 2.520 N CDC42BPA n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017002582.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.