Transcript: Human XM_017002949.2

PREDICTED: Homo sapiens centrosomal protein 170 (CEP170), transcript variant X30, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
CEP170 (9859)
Length:
6556
CDS:
234..4535

Additional Resources:

NCBI RefSeq record:
XM_017002949.2
NBCI Gene record:
CEP170 (9859)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017002949.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000296294 ACCAGATACTCCCAGTTATAA pLKO_005 2690 CDS 100% 15.000 10.500 N CEP170 n/a
2 TRCN0000296296 AGTTTCACTAGCCTCTATAAA pLKO_005 2811 CDS 100% 15.000 10.500 N CEP170 n/a
3 TRCN0000243676 ATAGTTTAGGTGGAGTATATT pLKO_005 5393 3UTR 100% 15.000 10.500 N Cep170 n/a
4 TRCN0000296262 TGACGTATAATGTAGTATATC pLKO_005 4680 3UTR 100% 13.200 9.240 N CEP170 n/a
5 TRCN0000123336 GCAGTCTCGTAGTGTGGATAA pLKO.1 335 CDS 100% 10.800 7.560 N CEP170 n/a
6 TRCN0000123334 GCAAACTAAATAGCCAACATT pLKO.1 4868 3UTR 100% 5.625 3.938 N CEP170 n/a
7 TRCN0000145016 GATGAAGTCATGGGAGATAAT pLKO.1 4005 CDS 100% 13.200 6.600 Y CEP170P1 n/a
8 TRCN0000142152 GCCGAAAGTGAAGTCCCTATT pLKO.1 4155 CDS 100% 10.800 5.400 Y CEP170P1 n/a
9 TRCN0000143509 GCTCTGAACAACATGGGATTT pLKO.1 4284 CDS 100% 10.800 5.400 Y CEP170P1 n/a
10 TRCN0000145071 CGTCTTTCAGTTCTCTAAGAA pLKO.1 4040 CDS 100% 5.625 2.813 Y CEP170P1 n/a
11 TRCN0000141633 CTCTTCATCCTGCTGCTGTTT pLKO.1 4408 CDS 100% 4.950 2.475 Y CEP170P1 n/a
12 TRCN0000144760 GAAGAGGAAGATGTTACAGTA pLKO.1 4506 CDS 100% 4.950 2.475 Y CEP170P1 n/a
13 TRCN0000144672 GAGAGATAGATTCAGTGACTT pLKO.1 3793 CDS 100% 4.950 2.475 Y CEP170P1 n/a
14 TRCN0000143391 GATCGGAATTGGGATGACATA pLKO.1 4119 CDS 100% 4.950 2.475 Y CEP170P1 n/a
15 TRCN0000142039 GCCGCAGCTGAATTTGAGAAT pLKO.1 4431 CDS 100% 4.950 2.475 Y CEP170P1 n/a
16 TRCN0000141173 CAGGAAGACTACATCCGAGAT pLKO.1 3693 CDS 100% 4.050 2.025 Y CEP170P1 n/a
17 TRCN0000123337 GCTGAATTTGAGAATGCTGAA pLKO.1 4437 CDS 100% 4.050 2.025 Y CEP170 n/a
18 TRCN0000144893 GCTGAATTTGAGAATGCTGAA pLKO.1 4437 CDS 100% 4.050 2.025 Y CEP170P1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017002949.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10401 pDONR223 100% 20.2% 20.1% None (many diffs) n/a
2 ccsbBroad304_10401 pLX_304 0% 20.2% 20.1% V5 (many diffs) n/a
3 TRCN0000481251 CGGCAAGATACATCCAGTGTCTTC pLX_317 52.5% 20.2% 20.1% V5 (many diffs) n/a
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